Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5113 and Pf6N2E2_5114 are separated by 260 nucleotides Pf6N2E2_5114 and Pf6N2E2_5115 overlap by 4 nucleotides Pf6N2E2_5115 and Pf6N2E2_5116 overlap by 4 nucleotides Pf6N2E2_5116 and Pf6N2E2_5117 are separated by 20 nucleotides
Pf6N2E2_5113 - Type IV fimbrial biogenesis protein FimT, at 5,896,822 to 5,897,337
_5113
Pf6N2E2_5114 - type 4 fimbrial biogenesis protein FimU, at 5,897,598 to 5,898,071
_5114
Pf6N2E2_5115 - Type IV fimbrial biogenesis protein PilV, at 5,898,068 to 5,898,529
_5115
Pf6N2E2_5116 - Type IV fimbrial biogenesis protein PilW, at 5,898,526 to 5,899,242
_5116
Pf6N2E2_5117 - Type IV fimbrial biogenesis protein PilX, at 5,899,263 to 5,899,733
_5117
Position (kb) 5898
5899 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5897.134 kb on + strand, within Pf6N2E2_5113 at 5897.135 kb on - strand, within Pf6N2E2_5113 at 5897.250 kb on + strand, within Pf6N2E2_5113 at 5897.250 kb on + strand, within Pf6N2E2_5113 at 5897.251 kb on - strand, within Pf6N2E2_5113 at 5897.251 kb on - strand at 5897.251 kb on - strand, within Pf6N2E2_5113 at 5897.251 kb on - strand, within Pf6N2E2_5113 at 5897.252 kb on + strand, within Pf6N2E2_5113 at 5897.252 kb on + strand, within Pf6N2E2_5113 at 5897.252 kb on + strand, within Pf6N2E2_5113 at 5897.252 kb on + strand, within Pf6N2E2_5113 at 5897.252 kb on + strand, within Pf6N2E2_5113 at 5897.253 kb on - strand, within Pf6N2E2_5113 at 5897.253 kb on - strand, within Pf6N2E2_5113 at 5897.253 kb on - strand, within Pf6N2E2_5113 at 5897.358 kb on + strand at 5897.359 kb on - strand at 5897.378 kb on + strand at 5897.378 kb on + strand at 5897.378 kb on + strand at 5897.379 kb on - strand at 5897.379 kb on - strand at 5897.379 kb on - strand at 5897.379 kb on - strand at 5897.379 kb on - strand at 5897.380 kb on + strand at 5897.464 kb on + strand at 5897.464 kb on + strand at 5897.464 kb on + strand at 5897.465 kb on - strand at 5897.465 kb on - strand at 5897.465 kb on - strand at 5897.465 kb on - strand at 5897.504 kb on + strand at 5897.504 kb on + strand at 5897.528 kb on - strand at 5897.528 kb on - strand at 5897.554 kb on + strand at 5897.554 kb on + strand at 5897.562 kb on - strand at 5897.562 kb on - strand at 5897.562 kb on - strand at 5897.571 kb on + strand at 5897.571 kb on + strand at 5897.572 kb on - strand at 5897.572 kb on - strand at 5897.572 kb on - strand at 5897.572 kb on - strand at 5897.572 kb on - strand at 5897.572 kb on - strand at 5897.573 kb on + strand at 5897.574 kb on - strand at 5897.574 kb on - strand at 5897.574 kb on - strand at 5897.610 kb on - strand at 5897.610 kb on - strand at 5897.610 kb on - strand at 5897.669 kb on + strand, within Pf6N2E2_5114 at 5897.670 kb on - strand, within Pf6N2E2_5114 at 5897.670 kb on - strand, within Pf6N2E2_5114 at 5897.844 kb on + strand, within Pf6N2E2_5114 at 5897.844 kb on + strand, within Pf6N2E2_5114 at 5897.844 kb on + strand, within Pf6N2E2_5114 at 5897.845 kb on - strand, within Pf6N2E2_5114 at 5897.845 kb on - strand, within Pf6N2E2_5114 at 5897.845 kb on - strand, within Pf6N2E2_5114 at 5897.845 kb on - strand, within Pf6N2E2_5114 at 5897.852 kb on + strand, within Pf6N2E2_5114 at 5897.872 kb on + strand, within Pf6N2E2_5114 at 5897.872 kb on + strand, within Pf6N2E2_5114 at 5897.872 kb on + strand, within Pf6N2E2_5114 at 5897.872 kb on + strand, within Pf6N2E2_5114 at 5897.937 kb on - strand, within Pf6N2E2_5114 at 5897.994 kb on + strand, within Pf6N2E2_5114 at 5897.994 kb on + strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.999 kb on - strand, within Pf6N2E2_5114 at 5897.999 kb on - strand, within Pf6N2E2_5114 at 5897.999 kb on - strand, within Pf6N2E2_5114 at 5898.050 kb on - strand at 5898.050 kb on - strand at 5898.188 kb on + strand, within Pf6N2E2_5115 at 5898.188 kb on + strand, within Pf6N2E2_5115 at 5898.189 kb on - strand, within Pf6N2E2_5115 at 5898.189 kb on - strand, within Pf6N2E2_5115 at 5898.234 kb on - strand, within Pf6N2E2_5115 at 5898.275 kb on + strand, within Pf6N2E2_5115 at 5898.275 kb on + strand, within Pf6N2E2_5115 at 5898.275 kb on + strand, within Pf6N2E2_5115 at 5898.276 kb on - strand, within Pf6N2E2_5115 at 5898.276 kb on - strand, within Pf6N2E2_5115 at 5898.408 kb on - strand, within Pf6N2E2_5115 at 5898.633 kb on + strand, within Pf6N2E2_5116 at 5898.634 kb on - strand, within Pf6N2E2_5116 at 5898.637 kb on + strand, within Pf6N2E2_5116 at 5898.637 kb on + strand, within Pf6N2E2_5116 at 5898.637 kb on + strand, within Pf6N2E2_5116 at 5898.637 kb on + strand, within Pf6N2E2_5116 at 5898.638 kb on - strand, within Pf6N2E2_5116 at 5898.638 kb on - strand, within Pf6N2E2_5116 at 5898.970 kb on + strand, within Pf6N2E2_5116 at 5898.970 kb on + strand, within Pf6N2E2_5116 at 5899.040 kb on + strand, within Pf6N2E2_5116 at 5899.112 kb on - strand, within Pf6N2E2_5116 at 5899.211 kb on + strand at 5899.211 kb on + strand at 5899.211 kb on + strand at 5899.212 kb on - strand at 5899.212 kb on - strand at 5899.212 kb on - strand at 5899.212 kb on - strand at 5899.212 kb on - strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.239 kb on - strand at 5899.239 kb on - strand at 5899.239 kb on - strand at 5899.239 kb on - strand at 5899.239 kb on - strand at 5899.239 kb on - strand at 5899.467 kb on + strand, within Pf6N2E2_5117 at 5899.467 kb on + strand, within Pf6N2E2_5117 at 5899.467 kb on + strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.484 kb on + strand, within Pf6N2E2_5117 at 5899.485 kb on - strand, within Pf6N2E2_5117
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 5,897,134 + Pf6N2E2_5113 0.60 +0.4 5,897,135 - Pf6N2E2_5113 0.61 -0.4 5,897,250 + Pf6N2E2_5113 0.83 +0.4 5,897,250 + Pf6N2E2_5113 0.83 -1.0 5,897,251 - Pf6N2E2_5113 0.83 +0.4 5,897,251 - +0.4 5,897,251 - Pf6N2E2_5113 0.83 +0.4 5,897,251 - Pf6N2E2_5113 0.83 +0.4 5,897,252 + Pf6N2E2_5113 0.83 +0.4 5,897,252 + Pf6N2E2_5113 0.83 -1.2 5,897,252 + Pf6N2E2_5113 0.83 -0.9 5,897,252 + Pf6N2E2_5113 0.83 -0.9 5,897,252 + Pf6N2E2_5113 0.83 -1.6 5,897,253 - Pf6N2E2_5113 0.84 +0.4 5,897,253 - Pf6N2E2_5113 0.84 +0.4 5,897,253 - Pf6N2E2_5113 0.84 -1.2 5,897,358 + -0.6 5,897,359 - -0.6 5,897,378 + +0.4 5,897,378 + -2.4 5,897,378 + +0.4 5,897,379 - -0.6 5,897,379 - +0.4 5,897,379 - -0.3 5,897,379 - -1.6 5,897,379 - -1.6 5,897,380 + -0.7 5,897,464 + +0.4 5,897,464 + -0.6 5,897,464 + -0.6 5,897,465 - -0.6 5,897,465 - +0.4 5,897,465 - +0.4 5,897,465 - -0.6 5,897,504 + +1.2 5,897,504 + +0.4 5,897,528 - +0.4 5,897,528 - -0.6 5,897,554 + -1.6 5,897,554 + +0.4 5,897,562 - -0.6 5,897,562 - -1.2 5,897,562 - +0.4 5,897,571 + -1.2 5,897,571 + -1.6 5,897,572 - +0.4 5,897,572 - +0.4 5,897,572 - +0.4 5,897,572 - -0.6 5,897,572 - -0.6 5,897,572 - -1.2 5,897,573 + -0.9 5,897,574 - +0.4 5,897,574 - -1.2 5,897,574 - +0.4 5,897,610 - -0.6 5,897,610 - -0.6 5,897,610 - +0.4 5,897,669 + Pf6N2E2_5114 0.15 -2.6 5,897,670 - Pf6N2E2_5114 0.15 -0.6 5,897,670 - Pf6N2E2_5114 0.15 -0.2 5,897,844 + Pf6N2E2_5114 0.52 +0.4 5,897,844 + Pf6N2E2_5114 0.52 +0.4 5,897,844 + Pf6N2E2_5114 0.52 +0.4 5,897,845 - Pf6N2E2_5114 0.52 -0.6 5,897,845 - Pf6N2E2_5114 0.52 -0.6 5,897,845 - Pf6N2E2_5114 0.52 +0.4 5,897,845 - Pf6N2E2_5114 0.52 +0.4 5,897,852 + Pf6N2E2_5114 0.54 +0.4 5,897,872 + Pf6N2E2_5114 0.58 -1.0 5,897,872 + Pf6N2E2_5114 0.58 +1.0 5,897,872 + Pf6N2E2_5114 0.58 -0.9 5,897,872 + Pf6N2E2_5114 0.58 +0.4 5,897,937 - Pf6N2E2_5114 0.72 +0.4 5,897,994 + Pf6N2E2_5114 0.84 +0.4 5,897,994 + Pf6N2E2_5114 0.84 -1.0 5,897,995 - Pf6N2E2_5114 0.84 -0.6 5,897,995 - Pf6N2E2_5114 0.84 -0.6 5,897,995 - Pf6N2E2_5114 0.84 +0.4 5,897,995 - Pf6N2E2_5114 0.84 +0.4 5,897,995 - Pf6N2E2_5114 0.84 +0.4 5,897,995 - Pf6N2E2_5114 0.84 +0.4 5,897,998 + Pf6N2E2_5114 0.84 +0.4 5,897,998 + Pf6N2E2_5114 0.84 -1.6 5,897,998 + Pf6N2E2_5114 0.84 +0.4 5,897,998 + Pf6N2E2_5114 0.84 -1.2 5,897,998 + Pf6N2E2_5114 0.84 +0.4 5,897,998 + Pf6N2E2_5114 0.84 +0.4 5,897,999 - Pf6N2E2_5114 0.85 -0.6 5,897,999 - Pf6N2E2_5114 0.85 +0.4 5,897,999 - Pf6N2E2_5114 0.85 -1.0 5,898,050 - -0.2 5,898,050 - -0.6 5,898,188 + Pf6N2E2_5115 0.26 -1.8 5,898,188 + Pf6N2E2_5115 0.26 -1.2 5,898,189 - Pf6N2E2_5115 0.26 +0.4 5,898,189 - Pf6N2E2_5115 0.26 +0.4 5,898,234 - Pf6N2E2_5115 0.36 +0.4 5,898,275 + Pf6N2E2_5115 0.45 +0.4 5,898,275 + Pf6N2E2_5115 0.45 -1.2 5,898,275 + Pf6N2E2_5115 0.45 -1.2 5,898,276 - Pf6N2E2_5115 0.45 -1.2 5,898,276 - Pf6N2E2_5115 0.45 -0.6 5,898,408 - Pf6N2E2_5115 0.74 -1.6 5,898,633 + Pf6N2E2_5116 0.15 -1.4 5,898,634 - Pf6N2E2_5116 0.15 -0.9 5,898,637 + Pf6N2E2_5116 0.15 -1.6 5,898,637 + Pf6N2E2_5116 0.15 -1.9 5,898,637 + Pf6N2E2_5116 0.15 +0.4 5,898,637 + Pf6N2E2_5116 0.15 +0.4 5,898,638 - Pf6N2E2_5116 0.16 +0.4 5,898,638 - Pf6N2E2_5116 0.16 +1.4 5,898,970 + Pf6N2E2_5116 0.62 +0.4 5,898,970 + Pf6N2E2_5116 0.62 +0.4 5,899,040 + Pf6N2E2_5116 0.72 +0.4 5,899,112 - Pf6N2E2_5116 0.82 +0.4 5,899,211 + +0.4 5,899,211 + -0.6 5,899,211 + +0.4 5,899,212 - -0.4 5,899,212 - +0.4 5,899,212 - -0.6 5,899,212 - -0.4 5,899,212 - -1.9 5,899,238 + +0.4 5,899,238 + +0.4 5,899,238 + +0.1 5,899,238 + -1.2 5,899,238 + +0.4 5,899,238 + +0.4 5,899,238 + -2.2 5,899,239 - +0.4 5,899,239 - +0.4 5,899,239 - +0.4 5,899,239 - -0.6 5,899,239 - -0.2 5,899,239 - +0.4 5,899,467 + Pf6N2E2_5117 0.43 -3.2 5,899,467 + Pf6N2E2_5117 0.43 +0.4 5,899,467 + Pf6N2E2_5117 0.43 -0.6 5,899,468 - Pf6N2E2_5117 0.44 -0.0 5,899,468 - Pf6N2E2_5117 0.44 +0.4 5,899,468 - Pf6N2E2_5117 0.44 -0.6 5,899,468 - Pf6N2E2_5117 0.44 +0.4 5,899,468 - Pf6N2E2_5117 0.44 -0.6 5,899,468 - Pf6N2E2_5117 0.44 -0.6 5,899,468 - Pf6N2E2_5117 0.44 +0.4 5,899,484 + Pf6N2E2_5117 0.47 -1.2 5,899,485 - Pf6N2E2_5117 0.47 +0.4
Or see this region's nucleotide sequence