Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5049 and Pf6N2E2_5050 overlap by 4 nucleotides Pf6N2E2_5050 and Pf6N2E2_5051 overlap by 9 nucleotides Pf6N2E2_5051 and Pf6N2E2_5052 overlap by 4 nucleotides Pf6N2E2_5052 and Pf6N2E2_5053 are separated by 70 nucleotides
Pf6N2E2_5049 - acetyltransferase, GNAT family, at 5,825,480 to 5,826,049
_5049
Pf6N2E2_5050 - hypothetical protein, at 5,826,046 to 5,826,162
_5050
Pf6N2E2_5051 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 5,826,154 to 5,827,023
_5051
Pf6N2E2_5052 - Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family, at 5,827,020 to 5,827,613
_5052
Pf6N2E2_5053 - putative exported protein, at 5,827,684 to 5,828,616
_5053
Position (kb)
5827
5828 Strain fitness (log2 ratio)
-2
-1
0
1 at 5826.030 kb on + strand at 5826.064 kb on + strand, within Pf6N2E2_5050 at 5826.064 kb on + strand, within Pf6N2E2_5050 at 5826.065 kb on - strand, within Pf6N2E2_5050 at 5826.065 kb on - strand, within Pf6N2E2_5050 at 5826.115 kb on + strand, within Pf6N2E2_5050 at 5826.116 kb on - strand, within Pf6N2E2_5050 at 5826.379 kb on + strand, within Pf6N2E2_5051 at 5826.380 kb on - strand, within Pf6N2E2_5051 at 5826.380 kb on - strand, within Pf6N2E2_5051 at 5826.481 kb on + strand, within Pf6N2E2_5051 at 5826.658 kb on + strand, within Pf6N2E2_5051 at 5826.659 kb on - strand, within Pf6N2E2_5051 at 5826.659 kb on - strand, within Pf6N2E2_5051 at 5826.659 kb on - strand, within Pf6N2E2_5051 at 5826.659 kb on - strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.689 kb on - strand, within Pf6N2E2_5051 at 5826.689 kb on - strand, within Pf6N2E2_5051 at 5826.689 kb on - strand, within Pf6N2E2_5051 at 5826.689 kb on - strand, within Pf6N2E2_5051 at 5826.742 kb on + strand, within Pf6N2E2_5051 at 5826.742 kb on + strand, within Pf6N2E2_5051 at 5826.742 kb on + strand, within Pf6N2E2_5051 at 5826.743 kb on - strand, within Pf6N2E2_5051 at 5826.743 kb on - strand, within Pf6N2E2_5051 at 5826.743 kb on - strand, within Pf6N2E2_5051 at 5826.743 kb on - strand, within Pf6N2E2_5051 at 5826.822 kb on + strand, within Pf6N2E2_5051 at 5826.822 kb on + strand, within Pf6N2E2_5051 at 5826.822 kb on + strand, within Pf6N2E2_5051 at 5826.822 kb on + strand, within Pf6N2E2_5051 at 5826.822 kb on + strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.893 kb on - strand, within Pf6N2E2_5051 at 5827.542 kb on - strand, within Pf6N2E2_5052 at 5827.658 kb on + strand at 5827.659 kb on - strand at 5827.805 kb on + strand, within Pf6N2E2_5053 at 5827.806 kb on - strand, within Pf6N2E2_5053 at 5827.916 kb on + strand, within Pf6N2E2_5053 at 5827.916 kb on + strand, within Pf6N2E2_5053 at 5827.916 kb on + strand, within Pf6N2E2_5053 at 5827.917 kb on - strand, within Pf6N2E2_5053 at 5827.937 kb on + strand, within Pf6N2E2_5053 at 5827.937 kb on + strand, within Pf6N2E2_5053 at 5827.937 kb on + strand, within Pf6N2E2_5053 at 5827.937 kb on + strand, within Pf6N2E2_5053 at 5827.937 kb on + strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5828.000 kb on + strand, within Pf6N2E2_5053 at 5828.066 kb on + strand, within Pf6N2E2_5053 at 5828.066 kb on + strand, within Pf6N2E2_5053 at 5828.066 kb on + strand, within Pf6N2E2_5053 at 5828.066 kb on + strand, within Pf6N2E2_5053 at 5828.067 kb on - strand, within Pf6N2E2_5053 at 5828.072 kb on + strand, within Pf6N2E2_5053 at 5828.242 kb on + strand, within Pf6N2E2_5053 at 5828.435 kb on + strand, within Pf6N2E2_5053 at 5828.435 kb on + strand, within Pf6N2E2_5053 at 5828.436 kb on - strand, within Pf6N2E2_5053 at 5828.436 kb on - strand, within Pf6N2E2_5053 at 5828.507 kb on - strand, within Pf6N2E2_5053
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 5,826,030 + -1.6 5,826,064 + Pf6N2E2_5050 0.15 -1.1 5,826,064 + Pf6N2E2_5050 0.15 +0.4 5,826,065 - Pf6N2E2_5050 0.16 -0.5 5,826,065 - Pf6N2E2_5050 0.16 +0.4 5,826,115 + Pf6N2E2_5050 0.59 -1.2 5,826,116 - Pf6N2E2_5050 0.60 +0.4 5,826,379 + Pf6N2E2_5051 0.26 -2.0 5,826,380 - Pf6N2E2_5051 0.26 +0.4 5,826,380 - Pf6N2E2_5051 0.26 +0.4 5,826,481 + Pf6N2E2_5051 0.38 -0.6 5,826,658 + Pf6N2E2_5051 0.58 +0.4 5,826,659 - Pf6N2E2_5051 0.58 -0.6 5,826,659 - Pf6N2E2_5051 0.58 +0.4 5,826,659 - Pf6N2E2_5051 0.58 +0.4 5,826,659 - Pf6N2E2_5051 0.58 -0.9 5,826,688 + Pf6N2E2_5051 0.61 +0.4 5,826,688 + Pf6N2E2_5051 0.61 +0.4 5,826,688 + Pf6N2E2_5051 0.61 -0.4 5,826,688 + Pf6N2E2_5051 0.61 +0.4 5,826,688 + Pf6N2E2_5051 0.61 +0.4 5,826,688 + Pf6N2E2_5051 0.61 +0.6 5,826,689 - Pf6N2E2_5051 0.61 +0.4 5,826,689 - Pf6N2E2_5051 0.61 -0.6 5,826,689 - Pf6N2E2_5051 0.61 -1.2 5,826,689 - Pf6N2E2_5051 0.61 -0.6 5,826,742 + Pf6N2E2_5051 0.68 +0.4 5,826,742 + Pf6N2E2_5051 0.68 -0.6 5,826,742 + Pf6N2E2_5051 0.68 +0.4 5,826,743 - Pf6N2E2_5051 0.68 +0.8 5,826,743 - Pf6N2E2_5051 0.68 +0.4 5,826,743 - Pf6N2E2_5051 0.68 -0.1 5,826,743 - Pf6N2E2_5051 0.68 -1.2 5,826,822 + Pf6N2E2_5051 0.77 -1.2 5,826,822 + Pf6N2E2_5051 0.77 +0.4 5,826,822 + Pf6N2E2_5051 0.77 -0.5 5,826,822 + Pf6N2E2_5051 0.77 +0.4 5,826,822 + Pf6N2E2_5051 0.77 -0.9 5,826,823 - Pf6N2E2_5051 0.77 -0.2 5,826,823 - Pf6N2E2_5051 0.77 -0.4 5,826,823 - Pf6N2E2_5051 0.77 -0.6 5,826,823 - Pf6N2E2_5051 0.77 -0.6 5,826,823 - Pf6N2E2_5051 0.77 +0.0 5,826,823 - Pf6N2E2_5051 0.77 -0.6 5,826,893 - Pf6N2E2_5051 0.85 -0.6 5,827,542 - Pf6N2E2_5052 0.88 -1.6 5,827,658 + -0.8 5,827,659 - -1.2 5,827,805 + Pf6N2E2_5053 0.13 -1.2 5,827,806 - Pf6N2E2_5053 0.13 -0.8 5,827,916 + Pf6N2E2_5053 0.25 -0.5 5,827,916 + Pf6N2E2_5053 0.25 -0.4 5,827,916 + Pf6N2E2_5053 0.25 -0.8 5,827,917 - Pf6N2E2_5053 0.25 +0.4 5,827,937 + Pf6N2E2_5053 0.27 -1.1 5,827,937 + Pf6N2E2_5053 0.27 -0.6 5,827,937 + Pf6N2E2_5053 0.27 +0.4 5,827,937 + Pf6N2E2_5053 0.27 +0.4 5,827,937 + Pf6N2E2_5053 0.27 +1.7 5,827,938 - Pf6N2E2_5053 0.27 -1.2 5,827,938 - Pf6N2E2_5053 0.27 +0.4 5,827,938 - Pf6N2E2_5053 0.27 -0.1 5,827,938 - Pf6N2E2_5053 0.27 +0.4 5,827,938 - Pf6N2E2_5053 0.27 +0.4 5,827,938 - Pf6N2E2_5053 0.27 -0.6 5,827,938 - Pf6N2E2_5053 0.27 -0.6 5,827,938 - Pf6N2E2_5053 0.27 -0.6 5,827,938 - Pf6N2E2_5053 0.27 -0.6 5,827,938 - Pf6N2E2_5053 0.27 -0.3 5,827,938 - Pf6N2E2_5053 0.27 +0.4 5,828,000 + Pf6N2E2_5053 0.34 -1.2 5,828,066 + Pf6N2E2_5053 0.41 +0.4 5,828,066 + Pf6N2E2_5053 0.41 -2.3 5,828,066 + Pf6N2E2_5053 0.41 -2.2 5,828,066 + Pf6N2E2_5053 0.41 +0.4 5,828,067 - Pf6N2E2_5053 0.41 -0.6 5,828,072 + Pf6N2E2_5053 0.42 -0.8 5,828,242 + Pf6N2E2_5053 0.60 -1.6 5,828,435 + Pf6N2E2_5053 0.80 +0.4 5,828,435 + Pf6N2E2_5053 0.80 +0.4 5,828,436 - Pf6N2E2_5053 0.81 -2.2 5,828,436 - Pf6N2E2_5053 0.81 -1.2 5,828,507 - Pf6N2E2_5053 0.88 -1.8
Or see this region's nucleotide sequence