Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3716

Experiment: Ying_Nucleobase14 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3715 and Pf6N2E2_3716 are separated by 146 nucleotidesPf6N2E2_3716 and Pf6N2E2_3717 overlap by 4 nucleotidesPf6N2E2_3717 and Pf6N2E2_3718 overlap by 14 nucleotides Pf6N2E2_3715 - FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase / (3R)-hydroxymyristoyl-[ACP] dehydratase (EC 4.2.1.-), at 4,327,782 to 4,329,461 _3715 Pf6N2E2_3716 - FIG143263: Glycosyl transferase, at 4,329,608 to 4,330,342 _3716 Pf6N2E2_3717 - Lysophospholipid acyltransferase, at 4,330,339 to 4,331,283 _3717 Pf6N2E2_3718 - Putative histidine ammonia-lyase protein, at 4,331,270 to 4,332,808 _3718 Position (kb) 4329 4330 4331Strain fitness (log2 ratio) -3 -2 -1 0 1at 4328.695 kb on - strand, within Pf6N2E2_3715at 4328.764 kb on + strand, within Pf6N2E2_3715at 4328.794 kb on + strand, within Pf6N2E2_3715at 4328.794 kb on + strand, within Pf6N2E2_3715at 4328.795 kb on - strand, within Pf6N2E2_3715at 4328.795 kb on - strand, within Pf6N2E2_3715at 4328.795 kb on - strand, within Pf6N2E2_3715at 4329.390 kb on + strandat 4329.391 kb on - strandat 4329.391 kb on - strandat 4329.439 kb on + strandat 4329.439 kb on + strandat 4329.440 kb on - strandat 4329.440 kb on - strandat 4329.440 kb on - strandat 4329.440 kb on - strandat 4329.459 kb on + strandat 4329.460 kb on - strandat 4329.528 kb on + strandat 4329.528 kb on + strandat 4329.529 kb on - strandat 4329.639 kb on - strandat 4329.639 kb on - strandat 4329.966 kb on - strand, within Pf6N2E2_3716at 4329.996 kb on - strand, within Pf6N2E2_3716at 4329.996 kb on - strand, within Pf6N2E2_3716at 4330.038 kb on + strand, within Pf6N2E2_3716at 4330.038 kb on + strand, within Pf6N2E2_3716at 4330.039 kb on - strand, within Pf6N2E2_3716at 4330.434 kb on - strand, within Pf6N2E2_3717at 4330.477 kb on + strand, within Pf6N2E2_3717at 4330.477 kb on + strand, within Pf6N2E2_3717at 4330.477 kb on + strand, within Pf6N2E2_3717at 4330.478 kb on - strand, within Pf6N2E2_3717at 4330.478 kb on - strand, within Pf6N2E2_3717at 4330.478 kb on - strand, within Pf6N2E2_3717at 4330.478 kb on - strand, within Pf6N2E2_3717at 4330.478 kb on - strand, within Pf6N2E2_3717at 4330.478 kb on - strand, within Pf6N2E2_3717at 4330.478 kb on - strand, within Pf6N2E2_3717at 4330.522 kb on + strand, within Pf6N2E2_3717at 4330.522 kb on + strand, within Pf6N2E2_3717at 4331.209 kb on + strandat 4331.209 kb on + strandat 4331.209 kb on + strandat 4331.209 kb on + strandat 4331.209 kb on + strandat 4331.210 kb on - strandat 4331.210 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3
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4,328,695 - Pf6N2E2_3715 0.54 -1.6
4,328,764 + Pf6N2E2_3715 0.58 +0.4
4,328,794 + Pf6N2E2_3715 0.60 +0.2
4,328,794 + Pf6N2E2_3715 0.60 +1.4
4,328,795 - Pf6N2E2_3715 0.60 -0.6
4,328,795 - Pf6N2E2_3715 0.60 +0.4
4,328,795 - Pf6N2E2_3715 0.60 -0.6
4,329,390 + +0.4
4,329,391 - -1.9
4,329,391 - +0.4
4,329,439 + -0.8
4,329,439 + +0.0
4,329,440 - +0.4
4,329,440 - -0.6
4,329,440 - -0.6
4,329,440 - +0.4
4,329,459 + -0.9
4,329,460 - -0.6
4,329,528 + -0.6
4,329,528 + +0.4
4,329,529 - +0.4
4,329,639 - -0.7
4,329,639 - -1.1
4,329,966 - Pf6N2E2_3716 0.49 -1.6
4,329,996 - Pf6N2E2_3716 0.53 +0.4
4,329,996 - Pf6N2E2_3716 0.53 -1.6
4,330,038 + Pf6N2E2_3716 0.59 -1.8
4,330,038 + Pf6N2E2_3716 0.59 -2.4
4,330,039 - Pf6N2E2_3716 0.59 -1.1
4,330,434 - Pf6N2E2_3717 0.10 -0.6
4,330,477 + Pf6N2E2_3717 0.15 -1.1
4,330,477 + Pf6N2E2_3717 0.15 -0.7
4,330,477 + Pf6N2E2_3717 0.15 -0.6
4,330,478 - Pf6N2E2_3717 0.15 +0.4
4,330,478 - Pf6N2E2_3717 0.15 -3.0
4,330,478 - Pf6N2E2_3717 0.15 +0.4
4,330,478 - Pf6N2E2_3717 0.15 +0.4
4,330,478 - Pf6N2E2_3717 0.15 +0.4
4,330,478 - Pf6N2E2_3717 0.15 +0.4
4,330,478 - Pf6N2E2_3717 0.15 -0.1
4,330,522 + Pf6N2E2_3717 0.19 +0.4
4,330,522 + Pf6N2E2_3717 0.19 -0.3
4,331,209 + +0.4
4,331,209 + -0.6
4,331,209 + +0.4
4,331,209 + -0.9
4,331,209 + +0.4
4,331,210 - +0.4
4,331,210 - -0.6

Or see this region's nucleotide sequence