Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6124 and Pf6N2E2_3462 are separated by 634 nucleotides Pf6N2E2_3462 and Pf6N2E2_3463 are separated by 71 nucleotides
Pf6N2E2_6124 - Small Subunit Ribosomal RNA; ssuRNA; SSU rRNA, at 4,040,400 to 4,041,928
_6124
Pf6N2E2_3462 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27), at 4,042,563 to 4,044,056
_3462
Pf6N2E2_3463 - Omega-amino acid--pyruvate aminotransferase (EC 2.6.1.18), at 4,044,128 to 4,045,498
_3463
Position (kb)
4042
4043
4044
4045 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4042.447 kb on + strand at 4042.557 kb on + strand at 4042.557 kb on + strand at 4042.557 kb on + strand at 4042.558 kb on - strand at 4042.564 kb on + strand at 4042.565 kb on - strand at 4042.645 kb on + strand at 4042.646 kb on - strand at 4042.646 kb on - strand at 4042.646 kb on - strand at 4042.720 kb on + strand, within Pf6N2E2_3462 at 4042.720 kb on + strand, within Pf6N2E2_3462 at 4042.721 kb on - strand, within Pf6N2E2_3462 at 4042.919 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.051 kb on + strand, within Pf6N2E2_3462 at 4043.051 kb on + strand, within Pf6N2E2_3462 at 4043.051 kb on + strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.053 kb on + strand, within Pf6N2E2_3462 at 4043.053 kb on + strand, within Pf6N2E2_3462 at 4043.053 kb on + strand, within Pf6N2E2_3462 at 4043.054 kb on - strand, within Pf6N2E2_3462 at 4043.054 kb on - strand, within Pf6N2E2_3462 at 4043.054 kb on - strand, within Pf6N2E2_3462 at 4043.054 kb on - strand, within Pf6N2E2_3462 at 4043.240 kb on - strand, within Pf6N2E2_3462 at 4043.359 kb on + strand, within Pf6N2E2_3462 at 4043.359 kb on + strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.365 kb on + strand, within Pf6N2E2_3462 at 4043.366 kb on - strand, within Pf6N2E2_3462 at 4043.366 kb on - strand, within Pf6N2E2_3462 at 4043.581 kb on + strand, within Pf6N2E2_3462 at 4043.582 kb on - strand, within Pf6N2E2_3462 at 4043.582 kb on - strand, within Pf6N2E2_3462 at 4043.582 kb on - strand, within Pf6N2E2_3462 at 4043.679 kb on + strand, within Pf6N2E2_3462 at 4043.679 kb on + strand, within Pf6N2E2_3462 at 4043.679 kb on + strand, within Pf6N2E2_3462 at 4043.680 kb on - strand, within Pf6N2E2_3462 at 4043.692 kb on + strand, within Pf6N2E2_3462 at 4043.813 kb on + strand, within Pf6N2E2_3462 at 4043.814 kb on - strand, within Pf6N2E2_3462 at 4043.882 kb on + strand, within Pf6N2E2_3462 at 4043.882 kb on + strand, within Pf6N2E2_3462 at 4043.882 kb on + strand, within Pf6N2E2_3462 at 4043.883 kb on - strand, within Pf6N2E2_3462 at 4043.883 kb on - strand, within Pf6N2E2_3462 at 4043.883 kb on - strand, within Pf6N2E2_3462 at 4043.961 kb on - strand at 4043.961 kb on - strand at 4044.072 kb on - strand at 4044.102 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.241 kb on - strand at 4044.241 kb on - strand at 4044.241 kb on - strand at 4044.337 kb on - strand, within Pf6N2E2_3463 at 4044.337 kb on - strand, within Pf6N2E2_3463 at 4044.337 kb on - strand, within Pf6N2E2_3463 at 4044.340 kb on + strand, within Pf6N2E2_3463 at 4044.340 kb on + strand, within Pf6N2E2_3463 at 4044.341 kb on - strand, within Pf6N2E2_3463 at 4044.341 kb on - strand, within Pf6N2E2_3463 at 4044.341 kb on - strand, within Pf6N2E2_3463 at 4044.341 kb on - strand, within Pf6N2E2_3463 at 4044.492 kb on + strand, within Pf6N2E2_3463 at 4044.492 kb on + strand, within Pf6N2E2_3463 at 4044.492 kb on + strand, within Pf6N2E2_3463 at 4044.493 kb on - strand, within Pf6N2E2_3463 at 4044.499 kb on - strand, within Pf6N2E2_3463 at 4044.555 kb on + strand, within Pf6N2E2_3463 at 4044.555 kb on + strand, within Pf6N2E2_3463 at 4044.555 kb on + strand, within Pf6N2E2_3463 at 4044.556 kb on - strand, within Pf6N2E2_3463 at 4044.754 kb on - strand, within Pf6N2E2_3463 at 4044.754 kb on - strand, within Pf6N2E2_3463 at 4044.754 kb on - strand, within Pf6N2E2_3463 at 4044.900 kb on + strand, within Pf6N2E2_3463 at 4044.900 kb on + strand, within Pf6N2E2_3463 at 4044.900 kb on + strand, within Pf6N2E2_3463 at 4045.021 kb on - strand, within Pf6N2E2_3463 at 4045.021 kb on - strand, within Pf6N2E2_3463
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 4,042,447 + -0.6 4,042,557 + +0.4 4,042,557 + +0.4 4,042,557 + +0.4 4,042,558 - -0.7 4,042,564 + -0.6 4,042,565 - -0.6 4,042,645 + -1.2 4,042,646 - -0.6 4,042,646 - -1.2 4,042,646 - -1.2 4,042,720 + Pf6N2E2_3462 0.11 +0.4 4,042,720 + Pf6N2E2_3462 0.11 +0.4 4,042,721 - Pf6N2E2_3462 0.11 -0.6 4,042,919 + Pf6N2E2_3462 0.24 +0.4 4,042,921 + Pf6N2E2_3462 0.24 -1.3 4,042,921 + Pf6N2E2_3462 0.24 +0.4 4,042,921 + Pf6N2E2_3462 0.24 +0.4 4,042,921 + Pf6N2E2_3462 0.24 +0.4 4,042,921 + Pf6N2E2_3462 0.24 +0.4 4,042,921 + Pf6N2E2_3462 0.24 +0.4 4,042,921 + Pf6N2E2_3462 0.24 -1.6 4,042,921 + Pf6N2E2_3462 0.24 -1.2 4,042,921 + Pf6N2E2_3462 0.24 -0.6 4,042,922 - Pf6N2E2_3462 0.24 -1.3 4,042,922 - Pf6N2E2_3462 0.24 -1.3 4,042,922 - Pf6N2E2_3462 0.24 -1.2 4,042,922 - Pf6N2E2_3462 0.24 -1.2 4,042,922 - Pf6N2E2_3462 0.24 -0.6 4,042,922 - Pf6N2E2_3462 0.24 +0.4 4,042,922 - Pf6N2E2_3462 0.24 -0.6 4,042,922 - Pf6N2E2_3462 0.24 -1.2 4,042,922 - Pf6N2E2_3462 0.24 +0.4 4,043,049 + Pf6N2E2_3462 0.33 +0.4 4,043,049 + Pf6N2E2_3462 0.33 +0.4 4,043,049 + Pf6N2E2_3462 0.33 +0.4 4,043,049 + Pf6N2E2_3462 0.33 -0.6 4,043,049 + Pf6N2E2_3462 0.33 -1.3 4,043,049 + Pf6N2E2_3462 0.33 -2.8 4,043,049 + Pf6N2E2_3462 0.33 +0.4 4,043,049 + Pf6N2E2_3462 0.33 +0.4 4,043,049 + Pf6N2E2_3462 0.33 -1.6 4,043,049 + Pf6N2E2_3462 0.33 -1.6 4,043,049 + Pf6N2E2_3462 0.33 +0.4 4,043,049 + Pf6N2E2_3462 0.33 -0.6 4,043,049 + Pf6N2E2_3462 0.33 +0.4 4,043,050 - Pf6N2E2_3462 0.33 -2.2 4,043,050 - Pf6N2E2_3462 0.33 +0.4 4,043,050 - Pf6N2E2_3462 0.33 -2.4 4,043,050 - Pf6N2E2_3462 0.33 -0.8 4,043,050 - Pf6N2E2_3462 0.33 -2.3 4,043,050 - Pf6N2E2_3462 0.33 +0.4 4,043,050 - Pf6N2E2_3462 0.33 +0.4 4,043,050 - Pf6N2E2_3462 0.33 -1.2 4,043,050 - Pf6N2E2_3462 0.33 -0.6 4,043,050 - Pf6N2E2_3462 0.33 -2.6 4,043,050 - Pf6N2E2_3462 0.33 -1.0 4,043,050 - Pf6N2E2_3462 0.33 -0.6 4,043,050 - Pf6N2E2_3462 0.33 -0.6 4,043,051 + Pf6N2E2_3462 0.33 -0.6 4,043,051 + Pf6N2E2_3462 0.33 -0.6 4,043,051 + Pf6N2E2_3462 0.33 -0.6 4,043,052 - Pf6N2E2_3462 0.33 -1.2 4,043,052 - Pf6N2E2_3462 0.33 -1.3 4,043,052 - Pf6N2E2_3462 0.33 -0.8 4,043,052 - Pf6N2E2_3462 0.33 -0.6 4,043,052 - Pf6N2E2_3462 0.33 -0.8 4,043,052 - Pf6N2E2_3462 0.33 -1.0 4,043,052 - Pf6N2E2_3462 0.33 -0.6 4,043,052 - Pf6N2E2_3462 0.33 -1.3 4,043,052 - Pf6N2E2_3462 0.33 -1.5 4,043,052 - Pf6N2E2_3462 0.33 +1.1 4,043,052 - Pf6N2E2_3462 0.33 -2.6 4,043,053 + Pf6N2E2_3462 0.33 -0.6 4,043,053 + Pf6N2E2_3462 0.33 +0.4 4,043,053 + Pf6N2E2_3462 0.33 +2.0 4,043,054 - Pf6N2E2_3462 0.33 +0.4 4,043,054 - Pf6N2E2_3462 0.33 -0.6 4,043,054 - Pf6N2E2_3462 0.33 +0.4 4,043,054 - Pf6N2E2_3462 0.33 -1.3 4,043,240 - Pf6N2E2_3462 0.45 -1.2 4,043,359 + Pf6N2E2_3462 0.53 -2.0 4,043,359 + Pf6N2E2_3462 0.53 +0.4 4,043,360 - Pf6N2E2_3462 0.53 -0.8 4,043,360 - Pf6N2E2_3462 0.53 +0.4 4,043,360 - Pf6N2E2_3462 0.53 +0.4 4,043,360 - Pf6N2E2_3462 0.53 -0.8 4,043,360 - Pf6N2E2_3462 0.53 -0.6 4,043,360 - Pf6N2E2_3462 0.53 -1.2 4,043,360 - Pf6N2E2_3462 0.53 -1.3 4,043,360 - Pf6N2E2_3462 0.53 -0.6 4,043,365 + Pf6N2E2_3462 0.54 +0.4 4,043,366 - Pf6N2E2_3462 0.54 +0.4 4,043,366 - Pf6N2E2_3462 0.54 +0.4 4,043,581 + Pf6N2E2_3462 0.68 -1.2 4,043,582 - Pf6N2E2_3462 0.68 -0.6 4,043,582 - Pf6N2E2_3462 0.68 +0.4 4,043,582 - Pf6N2E2_3462 0.68 -0.6 4,043,679 + Pf6N2E2_3462 0.75 -0.6 4,043,679 + Pf6N2E2_3462 0.75 +0.4 4,043,679 + Pf6N2E2_3462 0.75 +0.4 4,043,680 - Pf6N2E2_3462 0.75 +0.4 4,043,692 + Pf6N2E2_3462 0.76 -0.8 4,043,813 + Pf6N2E2_3462 0.84 +0.4 4,043,814 - Pf6N2E2_3462 0.84 +0.4 4,043,882 + Pf6N2E2_3462 0.88 -0.6 4,043,882 + Pf6N2E2_3462 0.88 +0.4 4,043,882 + Pf6N2E2_3462 0.88 -0.6 4,043,883 - Pf6N2E2_3462 0.88 -1.4 4,043,883 - Pf6N2E2_3462 0.88 -1.3 4,043,883 - Pf6N2E2_3462 0.88 -1.2 4,043,961 - +0.4 4,043,961 - +0.4 4,044,072 - -1.2 4,044,102 + -1.2 4,044,112 + -0.6 4,044,112 + +0.4 4,044,112 + -0.0 4,044,112 + -0.2 4,044,112 + +0.4 4,044,112 + +0.4 4,044,112 + -0.9 4,044,112 + +0.4 4,044,112 + +0.4 4,044,113 - -1.6 4,044,113 - +0.4 4,044,113 - +0.4 4,044,113 - -1.2 4,044,113 - -0.6 4,044,113 - -1.4 4,044,113 - -0.6 4,044,240 + +0.4 4,044,240 + +0.4 4,044,240 + +0.4 4,044,240 + -2.3 4,044,240 + -0.9 4,044,240 + -3.6 4,044,240 + -1.2 4,044,240 + -0.6 4,044,240 + -2.0 4,044,241 - -2.2 4,044,241 - +0.4 4,044,241 - +0.4 4,044,337 - Pf6N2E2_3463 0.15 -0.6 4,044,337 - Pf6N2E2_3463 0.15 -0.6 4,044,337 - Pf6N2E2_3463 0.15 +0.4 4,044,340 + Pf6N2E2_3463 0.15 -1.9 4,044,340 + Pf6N2E2_3463 0.15 -2.5 4,044,341 - Pf6N2E2_3463 0.16 +0.4 4,044,341 - Pf6N2E2_3463 0.16 -2.2 4,044,341 - Pf6N2E2_3463 0.16 +0.4 4,044,341 - Pf6N2E2_3463 0.16 +0.4 4,044,492 + Pf6N2E2_3463 0.27 -0.6 4,044,492 + Pf6N2E2_3463 0.27 -2.9 4,044,492 + Pf6N2E2_3463 0.27 +0.4 4,044,493 - Pf6N2E2_3463 0.27 +0.4 4,044,499 - Pf6N2E2_3463 0.27 +0.4 4,044,555 + Pf6N2E2_3463 0.31 +0.4 4,044,555 + Pf6N2E2_3463 0.31 -1.2 4,044,555 + Pf6N2E2_3463 0.31 +0.4 4,044,556 - Pf6N2E2_3463 0.31 -2.5 4,044,754 - Pf6N2E2_3463 0.46 -4.4 4,044,754 - Pf6N2E2_3463 0.46 -1.2 4,044,754 - Pf6N2E2_3463 0.46 -3.1 4,044,900 + Pf6N2E2_3463 0.56 -4.4 4,044,900 + Pf6N2E2_3463 0.56 -1.2 4,044,900 + Pf6N2E2_3463 0.56 +0.4 4,045,021 - Pf6N2E2_3463 0.65 +0.4 4,045,021 - Pf6N2E2_3463 0.65 +0.4
Or see this region's nucleotide sequence