Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3400 and Pf6N2E2_3401 are separated by 346 nucleotides Pf6N2E2_3401 and Pf6N2E2_3402 are separated by 26 nucleotides Pf6N2E2_3402 and Pf6N2E2_3403 overlap by 1 nucleotides
Pf6N2E2_3400 - hypothetical protein, at 3,944,817 to 3,945,611
_3400
Pf6N2E2_3401 - Translation initiation inhibitor, at 3,945,958 to 3,946,326
_3401
Pf6N2E2_3402 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 3,946,353 to 3,947,585
_3402
Pf6N2E2_3403 - Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7), at 3,947,585 to 3,949,012
_3403
Position (kb)
3946
3947
3948 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3945.492 kb on - strand, within Pf6N2E2_3400 at 3945.492 kb on - strand, within Pf6N2E2_3400 at 3945.492 kb on - strand, within Pf6N2E2_3400 at 3945.495 kb on + strand, within Pf6N2E2_3400 at 3945.528 kb on - strand, within Pf6N2E2_3400 at 3945.561 kb on - strand at 3945.578 kb on + strand at 3945.580 kb on + strand at 3945.580 kb on + strand at 3945.581 kb on - strand at 3945.581 kb on - strand at 3945.624 kb on + strand at 3945.625 kb on - strand at 3945.625 kb on - strand at 3945.625 kb on - strand at 3945.632 kb on + strand at 3945.632 kb on + strand at 3945.632 kb on + strand at 3945.632 kb on + strand at 3945.633 kb on - strand at 3945.633 kb on - strand at 3945.633 kb on - strand at 3945.633 kb on - strand at 3945.633 kb on - strand at 3945.710 kb on + strand at 3945.733 kb on + strand at 3945.751 kb on - strand at 3945.751 kb on - strand at 3945.751 kb on - strand at 3945.751 kb on - strand at 3945.751 kb on - strand at 3945.784 kb on - strand at 3945.852 kb on - strand at 3945.994 kb on - strand at 3946.056 kb on + strand, within Pf6N2E2_3401 at 3946.056 kb on + strand, within Pf6N2E2_3401 at 3946.056 kb on + strand, within Pf6N2E2_3401 at 3946.183 kb on + strand, within Pf6N2E2_3401 at 3946.183 kb on + strand, within Pf6N2E2_3401 at 3946.183 kb on + strand, within Pf6N2E2_3401 at 3946.183 kb on + strand, within Pf6N2E2_3401 at 3946.183 kb on + strand, within Pf6N2E2_3401 at 3946.184 kb on - strand, within Pf6N2E2_3401 at 3946.184 kb on - strand, within Pf6N2E2_3401 at 3946.184 kb on - strand, within Pf6N2E2_3401 at 3946.184 kb on - strand, within Pf6N2E2_3401 at 3946.184 kb on - strand, within Pf6N2E2_3401 at 3946.256 kb on + strand, within Pf6N2E2_3401 at 3946.256 kb on + strand, within Pf6N2E2_3401 at 3946.256 kb on + strand, within Pf6N2E2_3401 at 3946.256 kb on + strand, within Pf6N2E2_3401 at 3946.256 kb on + strand, within Pf6N2E2_3401 at 3946.257 kb on - strand, within Pf6N2E2_3401 at 3946.257 kb on - strand, within Pf6N2E2_3401 at 3946.257 kb on - strand, within Pf6N2E2_3401 at 3946.257 kb on - strand, within Pf6N2E2_3401 at 3946.333 kb on + strand at 3946.334 kb on - strand at 3946.354 kb on + strand at 3946.355 kb on - strand at 3946.398 kb on - strand at 3946.444 kb on + strand at 3946.444 kb on + strand at 3946.445 kb on - strand at 3946.445 kb on - strand at 3946.674 kb on - strand, within Pf6N2E2_3402 at 3946.674 kb on - strand, within Pf6N2E2_3402 at 3946.733 kb on + strand, within Pf6N2E2_3402 at 3946.733 kb on + strand, within Pf6N2E2_3402 at 3946.733 kb on + strand, within Pf6N2E2_3402 at 3946.734 kb on - strand, within Pf6N2E2_3402 at 3946.734 kb on - strand, within Pf6N2E2_3402 at 3946.734 kb on - strand, within Pf6N2E2_3402 at 3946.734 kb on - strand, within Pf6N2E2_3402 at 3946.742 kb on - strand, within Pf6N2E2_3402 at 3946.785 kb on + strand, within Pf6N2E2_3402 at 3946.786 kb on - strand, within Pf6N2E2_3402 at 3946.786 kb on - strand, within Pf6N2E2_3402 at 3946.786 kb on - strand, within Pf6N2E2_3402 at 3946.802 kb on - strand, within Pf6N2E2_3402 at 3947.036 kb on + strand, within Pf6N2E2_3402 at 3947.036 kb on + strand, within Pf6N2E2_3402 at 3947.037 kb on - strand, within Pf6N2E2_3402 at 3947.037 kb on - strand, within Pf6N2E2_3402 at 3947.037 kb on - strand, within Pf6N2E2_3402 at 3947.037 kb on - strand, within Pf6N2E2_3402 at 3947.037 kb on - strand, within Pf6N2E2_3402 at 3947.039 kb on - strand, within Pf6N2E2_3402 at 3947.294 kb on + strand, within Pf6N2E2_3402 at 3947.295 kb on - strand, within Pf6N2E2_3402 at 3947.295 kb on - strand, within Pf6N2E2_3402 at 3947.434 kb on + strand, within Pf6N2E2_3402 at 3947.434 kb on + strand, within Pf6N2E2_3402 at 3947.434 kb on + strand, within Pf6N2E2_3402 at 3947.434 kb on + strand, within Pf6N2E2_3402 at 3947.434 kb on + strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.441 kb on - strand, within Pf6N2E2_3402 at 3947.441 kb on - strand, within Pf6N2E2_3402 at 3947.441 kb on - strand, within Pf6N2E2_3402 at 3947.513 kb on + strand at 3947.514 kb on - strand at 3947.514 kb on - strand at 3947.530 kb on - strand at 3947.656 kb on - strand at 3947.656 kb on - strand at 3947.814 kb on + strand, within Pf6N2E2_3403 at 3947.814 kb on + strand, within Pf6N2E2_3403 at 3947.815 kb on - strand, within Pf6N2E2_3403 at 3947.815 kb on - strand, within Pf6N2E2_3403 at 3947.815 kb on - strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.941 kb on - strand, within Pf6N2E2_3403 at 3947.941 kb on - strand, within Pf6N2E2_3403 at 3947.941 kb on - strand, within Pf6N2E2_3403 at 3948.002 kb on + strand, within Pf6N2E2_3403 at 3948.002 kb on + strand, within Pf6N2E2_3403 at 3948.003 kb on - strand, within Pf6N2E2_3403 at 3948.003 kb on - strand, within Pf6N2E2_3403 at 3948.003 kb on - strand, within Pf6N2E2_3403 at 3948.039 kb on + strand, within Pf6N2E2_3403 at 3948.040 kb on - strand, within Pf6N2E2_3403 at 3948.056 kb on - strand, within Pf6N2E2_3403 at 3948.056 kb on - strand, within Pf6N2E2_3403 at 3948.156 kb on + strand, within Pf6N2E2_3403 at 3948.156 kb on + strand, within Pf6N2E2_3403 at 3948.157 kb on - strand, within Pf6N2E2_3403 at 3948.157 kb on - strand, within Pf6N2E2_3403 at 3948.157 kb on - strand, within Pf6N2E2_3403 at 3948.157 kb on - strand, within Pf6N2E2_3403 at 3948.157 kb on - strand, within Pf6N2E2_3403 at 3948.168 kb on - strand, within Pf6N2E2_3403 at 3948.168 kb on - strand, within Pf6N2E2_3403 at 3948.215 kb on - strand, within Pf6N2E2_3403 at 3948.215 kb on - strand, within Pf6N2E2_3403 at 3948.347 kb on - strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.555 kb on - strand, within Pf6N2E2_3403 at 3948.555 kb on - strand, within Pf6N2E2_3403 at 3948.555 kb on - strand, within Pf6N2E2_3403 at 3948.555 kb on - strand, within Pf6N2E2_3403
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 3,945,492 - Pf6N2E2_3400 0.85 -0.7 3,945,492 - Pf6N2E2_3400 0.85 -1.1 3,945,492 - Pf6N2E2_3400 0.85 +0.4 3,945,495 + Pf6N2E2_3400 0.85 +0.4 3,945,528 - Pf6N2E2_3400 0.89 +0.4 3,945,561 - -0.6 3,945,578 + +0.4 3,945,580 + -1.2 3,945,580 + +0.4 3,945,581 - -0.5 3,945,581 - -0.9 3,945,624 + +0.4 3,945,625 - +0.7 3,945,625 - -0.6 3,945,625 - +0.4 3,945,632 + +0.4 3,945,632 + -1.4 3,945,632 + +0.4 3,945,632 + -0.6 3,945,633 - +0.4 3,945,633 - +0.4 3,945,633 - +0.4 3,945,633 - +0.4 3,945,633 - -0.6 3,945,710 + +0.4 3,945,733 + +0.4 3,945,751 - -0.8 3,945,751 - +0.4 3,945,751 - +0.4 3,945,751 - -0.6 3,945,751 - +0.4 3,945,784 - -0.6 3,945,852 - -2.2 3,945,994 - -0.6 3,946,056 + Pf6N2E2_3401 0.27 +0.4 3,946,056 + Pf6N2E2_3401 0.27 +1.4 3,946,056 + Pf6N2E2_3401 0.27 -0.6 3,946,183 + Pf6N2E2_3401 0.61 -0.2 3,946,183 + Pf6N2E2_3401 0.61 -0.6 3,946,183 + Pf6N2E2_3401 0.61 -0.6 3,946,183 + Pf6N2E2_3401 0.61 -0.7 3,946,183 + Pf6N2E2_3401 0.61 -0.6 3,946,184 - Pf6N2E2_3401 0.61 +0.4 3,946,184 - Pf6N2E2_3401 0.61 -0.6 3,946,184 - Pf6N2E2_3401 0.61 +0.4 3,946,184 - Pf6N2E2_3401 0.61 -2.1 3,946,184 - Pf6N2E2_3401 0.61 -1.3 3,946,256 + Pf6N2E2_3401 0.81 -0.6 3,946,256 + Pf6N2E2_3401 0.81 +0.4 3,946,256 + Pf6N2E2_3401 0.81 -1.6 3,946,256 + Pf6N2E2_3401 0.81 -0.6 3,946,256 + Pf6N2E2_3401 0.81 +0.4 3,946,257 - Pf6N2E2_3401 0.81 +0.4 3,946,257 - Pf6N2E2_3401 0.81 -1.0 3,946,257 - Pf6N2E2_3401 0.81 -0.6 3,946,257 - Pf6N2E2_3401 0.81 +0.4 3,946,333 + -1.2 3,946,334 - +0.4 3,946,354 + +0.4 3,946,355 - +0.4 3,946,398 - +0.4 3,946,444 + +0.4 3,946,444 + +0.4 3,946,445 - -0.5 3,946,445 - +0.4 3,946,674 - Pf6N2E2_3402 0.26 -1.4 3,946,674 - Pf6N2E2_3402 0.26 +0.4 3,946,733 + Pf6N2E2_3402 0.31 -0.6 3,946,733 + Pf6N2E2_3402 0.31 +0.4 3,946,733 + Pf6N2E2_3402 0.31 -0.8 3,946,734 - Pf6N2E2_3402 0.31 -1.6 3,946,734 - Pf6N2E2_3402 0.31 -0.6 3,946,734 - Pf6N2E2_3402 0.31 -0.8 3,946,734 - Pf6N2E2_3402 0.31 -1.9 3,946,742 - Pf6N2E2_3402 0.32 -0.6 3,946,785 + Pf6N2E2_3402 0.35 +0.4 3,946,786 - Pf6N2E2_3402 0.35 +0.4 3,946,786 - Pf6N2E2_3402 0.35 -0.7 3,946,786 - Pf6N2E2_3402 0.35 +0.4 3,946,802 - Pf6N2E2_3402 0.36 -0.6 3,947,036 + Pf6N2E2_3402 0.55 -1.6 3,947,036 + Pf6N2E2_3402 0.55 -0.6 3,947,037 - Pf6N2E2_3402 0.55 -0.6 3,947,037 - Pf6N2E2_3402 0.55 +0.4 3,947,037 - Pf6N2E2_3402 0.55 +0.4 3,947,037 - Pf6N2E2_3402 0.55 +0.4 3,947,037 - Pf6N2E2_3402 0.55 -0.6 3,947,039 - Pf6N2E2_3402 0.56 -2.9 3,947,294 + Pf6N2E2_3402 0.76 +0.4 3,947,295 - Pf6N2E2_3402 0.76 +0.4 3,947,295 - Pf6N2E2_3402 0.76 +0.4 3,947,434 + Pf6N2E2_3402 0.88 -0.1 3,947,434 + Pf6N2E2_3402 0.88 -0.8 3,947,434 + Pf6N2E2_3402 0.88 +0.4 3,947,434 + Pf6N2E2_3402 0.88 +0.4 3,947,434 + Pf6N2E2_3402 0.88 -0.6 3,947,435 - Pf6N2E2_3402 0.88 -0.3 3,947,435 - Pf6N2E2_3402 0.88 -0.6 3,947,435 - Pf6N2E2_3402 0.88 +0.4 3,947,435 - Pf6N2E2_3402 0.88 +0.4 3,947,435 - Pf6N2E2_3402 0.88 -0.9 3,947,435 - Pf6N2E2_3402 0.88 +0.4 3,947,441 - Pf6N2E2_3402 0.88 -0.6 3,947,441 - Pf6N2E2_3402 0.88 +0.4 3,947,441 - Pf6N2E2_3402 0.88 +0.4 3,947,513 + -0.9 3,947,514 - +0.4 3,947,514 - +0.4 3,947,530 - +0.4 3,947,656 - -1.2 3,947,656 - -1.6 3,947,814 + Pf6N2E2_3403 0.16 -0.7 3,947,814 + Pf6N2E2_3403 0.16 -1.2 3,947,815 - Pf6N2E2_3403 0.16 +0.4 3,947,815 - Pf6N2E2_3403 0.16 +0.4 3,947,815 - Pf6N2E2_3403 0.16 -0.6 3,947,940 + Pf6N2E2_3403 0.25 -0.6 3,947,940 + Pf6N2E2_3403 0.25 -1.2 3,947,940 + Pf6N2E2_3403 0.25 +0.4 3,947,940 + Pf6N2E2_3403 0.25 +0.7 3,947,940 + Pf6N2E2_3403 0.25 +0.4 3,947,940 + Pf6N2E2_3403 0.25 -1.2 3,947,941 - Pf6N2E2_3403 0.25 -2.2 3,947,941 - Pf6N2E2_3403 0.25 -0.6 3,947,941 - Pf6N2E2_3403 0.25 -0.2 3,948,002 + Pf6N2E2_3403 0.29 +0.4 3,948,002 + Pf6N2E2_3403 0.29 -0.6 3,948,003 - Pf6N2E2_3403 0.29 -1.2 3,948,003 - Pf6N2E2_3403 0.29 +0.4 3,948,003 - Pf6N2E2_3403 0.29 -0.6 3,948,039 + Pf6N2E2_3403 0.32 +0.7 3,948,040 - Pf6N2E2_3403 0.32 +0.4 3,948,056 - Pf6N2E2_3403 0.33 -1.2 3,948,056 - Pf6N2E2_3403 0.33 -0.5 3,948,156 + Pf6N2E2_3403 0.40 +0.4 3,948,156 + Pf6N2E2_3403 0.40 -1.2 3,948,157 - Pf6N2E2_3403 0.40 -0.6 3,948,157 - Pf6N2E2_3403 0.40 +0.4 3,948,157 - Pf6N2E2_3403 0.40 -0.4 3,948,157 - Pf6N2E2_3403 0.40 +0.4 3,948,157 - Pf6N2E2_3403 0.40 -0.6 3,948,168 - Pf6N2E2_3403 0.41 -0.6 3,948,168 - Pf6N2E2_3403 0.41 -1.3 3,948,215 - Pf6N2E2_3403 0.44 -0.6 3,948,215 - Pf6N2E2_3403 0.44 -1.6 3,948,347 - Pf6N2E2_3403 0.53 +0.4 3,948,554 + Pf6N2E2_3403 0.68 -1.9 3,948,554 + Pf6N2E2_3403 0.68 +0.4 3,948,554 + Pf6N2E2_3403 0.68 +0.4 3,948,554 + Pf6N2E2_3403 0.68 -0.9 3,948,554 + Pf6N2E2_3403 0.68 -1.2 3,948,554 + Pf6N2E2_3403 0.68 -0.6 3,948,554 + Pf6N2E2_3403 0.68 -1.6 3,948,555 - Pf6N2E2_3403 0.68 -0.8 3,948,555 - Pf6N2E2_3403 0.68 +0.4 3,948,555 - Pf6N2E2_3403 0.68 +0.4 3,948,555 - Pf6N2E2_3403 0.68 +0.4
Or see this region's nucleotide sequence