Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2927 and Pf6N2E2_2928 are separated by 27 nucleotides Pf6N2E2_2928 and Pf6N2E2_2929 overlap by 4 nucleotides Pf6N2E2_2929 and Pf6N2E2_2930 overlap by 4 nucleotides Pf6N2E2_2930 and Pf6N2E2_2931 are separated by 84 nucleotides
Pf6N2E2_2927 - Putative signal peptide protein, at 3,420,173 to 3,420,478
_2927
Pf6N2E2_2928 - Uncharacterized protein conserved in bacteria, NMA0228-like, at 3,420,506 to 3,421,375
_2928
Pf6N2E2_2929 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,421,372 to 3,422,130
_2929
Pf6N2E2_2930 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 3,422,127 to 3,422,606
_2930
Pf6N2E2_2931 - Transcriptional regulator, LysR family, at 3,422,691 to 3,423,647
_2931
Position (kb)
3421
3422
3423 Strain fitness (log2 ratio)
-2
-1
0
1 at 3420.514 kb on + strand at 3420.591 kb on + strand at 3420.592 kb on - strand at 3420.592 kb on - strand at 3420.641 kb on + strand, within Pf6N2E2_2928 at 3420.641 kb on + strand, within Pf6N2E2_2928 at 3420.641 kb on + strand, within Pf6N2E2_2928 at 3420.642 kb on - strand, within Pf6N2E2_2928 at 3420.642 kb on - strand, within Pf6N2E2_2928 at 3420.642 kb on - strand, within Pf6N2E2_2928 at 3420.642 kb on - strand, within Pf6N2E2_2928 at 3420.642 kb on - strand, within Pf6N2E2_2928 at 3420.872 kb on + strand, within Pf6N2E2_2928 at 3420.873 kb on - strand, within Pf6N2E2_2928 at 3420.938 kb on - strand, within Pf6N2E2_2928 at 3420.938 kb on - strand, within Pf6N2E2_2928 at 3420.965 kb on + strand, within Pf6N2E2_2928 at 3420.965 kb on + strand, within Pf6N2E2_2928 at 3420.966 kb on - strand, within Pf6N2E2_2928 at 3420.966 kb on - strand, within Pf6N2E2_2928 at 3421.064 kb on + strand, within Pf6N2E2_2928 at 3421.064 kb on + strand, within Pf6N2E2_2928 at 3421.065 kb on - strand, within Pf6N2E2_2928 at 3421.194 kb on + strand, within Pf6N2E2_2928 at 3421.238 kb on + strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.586 kb on - strand, within Pf6N2E2_2929 at 3421.684 kb on + strand, within Pf6N2E2_2929 at 3421.685 kb on - strand, within Pf6N2E2_2929 at 3421.685 kb on - strand, within Pf6N2E2_2929 at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.102 kb on - strand at 3422.102 kb on - strand at 3422.108 kb on + strand at 3422.108 kb on + strand at 3422.108 kb on + strand at 3422.109 kb on - strand at 3422.121 kb on - strand at 3422.121 kb on - strand at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.536 kb on - strand, within Pf6N2E2_2930 at 3422.620 kb on + strand at 3422.621 kb on - strand at 3422.621 kb on - strand at 3422.621 kb on - strand at 3422.621 kb on - strand at 3422.693 kb on - strand at 3422.747 kb on - strand at 3422.747 kb on - strand at 3422.788 kb on + strand, within Pf6N2E2_2931 at 3422.803 kb on + strand, within Pf6N2E2_2931 at 3422.803 kb on + strand, within Pf6N2E2_2931 at 3422.803 kb on + strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.938 kb on + strand, within Pf6N2E2_2931 at 3422.938 kb on + strand, within Pf6N2E2_2931 at 3422.939 kb on - strand, within Pf6N2E2_2931 at 3422.939 kb on - strand, within Pf6N2E2_2931 at 3422.946 kb on - strand, within Pf6N2E2_2931 at 3422.998 kb on + strand, within Pf6N2E2_2931 at 3422.999 kb on - strand, within Pf6N2E2_2931 at 3422.999 kb on - strand, within Pf6N2E2_2931 at 3422.999 kb on - strand, within Pf6N2E2_2931 at 3423.022 kb on + strand, within Pf6N2E2_2931 at 3423.022 kb on + strand, within Pf6N2E2_2931 at 3423.023 kb on - strand, within Pf6N2E2_2931 at 3423.023 kb on - strand, within Pf6N2E2_2931 at 3423.029 kb on + strand, within Pf6N2E2_2931 at 3423.082 kb on + strand, within Pf6N2E2_2931
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 3,420,514 + -0.6 3,420,591 + -1.7 3,420,592 - +0.3 3,420,592 - -0.9 3,420,641 + Pf6N2E2_2928 0.16 +0.3 3,420,641 + Pf6N2E2_2928 0.16 +0.3 3,420,641 + Pf6N2E2_2928 0.16 -2.0 3,420,642 - Pf6N2E2_2928 0.16 +0.3 3,420,642 - Pf6N2E2_2928 0.16 -0.7 3,420,642 - Pf6N2E2_2928 0.16 +0.3 3,420,642 - Pf6N2E2_2928 0.16 -1.7 3,420,642 - Pf6N2E2_2928 0.16 -0.6 3,420,872 + Pf6N2E2_2928 0.42 +0.3 3,420,873 - Pf6N2E2_2928 0.42 +0.3 3,420,938 - Pf6N2E2_2928 0.50 -1.3 3,420,938 - Pf6N2E2_2928 0.50 +0.3 3,420,965 + Pf6N2E2_2928 0.53 -1.3 3,420,965 + Pf6N2E2_2928 0.53 -0.4 3,420,966 - Pf6N2E2_2928 0.53 -0.7 3,420,966 - Pf6N2E2_2928 0.53 +0.3 3,421,064 + Pf6N2E2_2928 0.64 +0.3 3,421,064 + Pf6N2E2_2928 0.64 +0.3 3,421,065 - Pf6N2E2_2928 0.64 +0.3 3,421,194 + Pf6N2E2_2928 0.79 -0.4 3,421,238 + Pf6N2E2_2928 0.84 +0.0 3,421,239 - Pf6N2E2_2928 0.84 -0.7 3,421,239 - Pf6N2E2_2928 0.84 +0.3 3,421,239 - Pf6N2E2_2928 0.84 -1.9 3,421,239 - Pf6N2E2_2928 0.84 +0.3 3,421,239 - Pf6N2E2_2928 0.84 -1.0 3,421,239 - Pf6N2E2_2928 0.84 -0.7 3,421,585 + Pf6N2E2_2929 0.28 +0.3 3,421,585 + Pf6N2E2_2929 0.28 -0.7 3,421,585 + Pf6N2E2_2929 0.28 -0.7 3,421,585 + Pf6N2E2_2929 0.28 -0.7 3,421,586 - Pf6N2E2_2929 0.28 -0.7 3,421,684 + Pf6N2E2_2929 0.41 +0.3 3,421,685 - Pf6N2E2_2929 0.41 +0.3 3,421,685 - Pf6N2E2_2929 0.41 -1.9 3,422,101 + -1.0 3,422,101 + +0.9 3,422,101 + +0.3 3,422,101 + -0.7 3,422,102 - +0.3 3,422,102 - -0.7 3,422,108 + +0.3 3,422,108 + -0.7 3,422,108 + -0.4 3,422,109 - +0.8 3,422,121 - +0.3 3,422,121 - +0.3 3,422,513 + Pf6N2E2_2930 0.80 +0.3 3,422,513 + Pf6N2E2_2930 0.80 +0.7 3,422,513 + Pf6N2E2_2930 0.80 +0.3 3,422,513 + Pf6N2E2_2930 0.80 -0.7 3,422,513 + Pf6N2E2_2930 0.80 -0.7 3,422,536 - Pf6N2E2_2930 0.85 -0.7 3,422,620 + +0.3 3,422,621 - +0.3 3,422,621 - +0.3 3,422,621 - -0.7 3,422,621 - -1.7 3,422,693 - -0.0 3,422,747 - -0.9 3,422,747 - +0.3 3,422,788 + Pf6N2E2_2931 0.10 -1.5 3,422,803 + Pf6N2E2_2931 0.12 +0.3 3,422,803 + Pf6N2E2_2931 0.12 -1.3 3,422,803 + Pf6N2E2_2931 0.12 -0.7 3,422,804 - Pf6N2E2_2931 0.12 -0.7 3,422,804 - Pf6N2E2_2931 0.12 -0.7 3,422,804 - Pf6N2E2_2931 0.12 -0.7 3,422,804 - Pf6N2E2_2931 0.12 -1.5 3,422,804 - Pf6N2E2_2931 0.12 -0.9 3,422,938 + Pf6N2E2_2931 0.26 +0.3 3,422,938 + Pf6N2E2_2931 0.26 -1.3 3,422,939 - Pf6N2E2_2931 0.26 -0.4 3,422,939 - Pf6N2E2_2931 0.26 +0.3 3,422,946 - Pf6N2E2_2931 0.27 +0.3 3,422,998 + Pf6N2E2_2931 0.32 +0.3 3,422,999 - Pf6N2E2_2931 0.32 +0.3 3,422,999 - Pf6N2E2_2931 0.32 -0.7 3,422,999 - Pf6N2E2_2931 0.32 -0.7 3,423,022 + Pf6N2E2_2931 0.35 +0.3 3,423,022 + Pf6N2E2_2931 0.35 -0.7 3,423,023 - Pf6N2E2_2931 0.35 -2.0 3,423,023 - Pf6N2E2_2931 0.35 -0.7 3,423,029 + Pf6N2E2_2931 0.35 +0.3 3,423,082 + Pf6N2E2_2931 0.41 +0.3
Or see this region's nucleotide sequence