Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2287 and Pf6N2E2_2288 are separated by 115 nucleotides Pf6N2E2_2288 and Pf6N2E2_2289 are separated by 108 nucleotides
Pf6N2E2_2287 - DNA topoisomerase I (EC 5.99.1.2), at 2,704,969 to 2,707,581
_2287
Pf6N2E2_2288 - FIG00953423: hypothetical protein, at 2,707,697 to 2,707,933
_2288
Pf6N2E2_2289 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 2,708,042 to 2,709,193
_2289
Position (kb)
2707
2708 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2707.644 kb on - strand at 2707.644 kb on - strand at 2707.644 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.650 kb on - strand at 2707.650 kb on - strand at 2707.650 kb on - strand at 2707.650 kb on - strand at 2707.833 kb on + strand, within Pf6N2E2_2288 at 2707.880 kb on + strand, within Pf6N2E2_2288 at 2707.902 kb on + strand, within Pf6N2E2_2288 at 2707.902 kb on + strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.911 kb on + strand at 2707.925 kb on + strand at 2707.925 kb on + strand at 2707.926 kb on - strand at 2707.982 kb on - strand at 2707.984 kb on + strand at 2707.985 kb on - strand at 2707.985 kb on - strand at 2708.043 kb on + strand at 2708.044 kb on - strand at 2708.044 kb on - strand at 2708.362 kb on - strand, within Pf6N2E2_2289 at 2708.791 kb on - strand, within Pf6N2E2_2289
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 2,707,644 - +0.3 2,707,644 - +0.3 2,707,644 - +0.3 2,707,646 - +0.3 2,707,646 - -0.7 2,707,646 - -3.1 2,707,646 - -0.7 2,707,646 - -0.7 2,707,646 - +0.3 2,707,646 - -0.3 2,707,646 - +0.3 2,707,646 - -0.3 2,707,646 - +0.3 2,707,646 - -0.7 2,707,646 - -1.3 2,707,646 - -0.7 2,707,646 - +0.3 2,707,646 - -1.3 2,707,646 - -1.9 2,707,646 - -0.5 2,707,646 - -0.7 2,707,646 - +0.3 2,707,646 - +0.3 2,707,648 - -0.7 2,707,648 - +0.3 2,707,648 - -0.8 2,707,648 - -1.0 2,707,648 - -0.7 2,707,648 - -0.7 2,707,648 - +0.3 2,707,648 - -1.7 2,707,648 - -0.7 2,707,648 - +0.3 2,707,648 - -1.5 2,707,648 - -1.3 2,707,648 - -0.7 2,707,648 - -0.7 2,707,648 - -1.5 2,707,650 - -0.5 2,707,650 - -1.3 2,707,650 - -0.1 2,707,650 - -1.1 2,707,833 + Pf6N2E2_2288 0.57 -0.7 2,707,880 + Pf6N2E2_2288 0.77 +0.3 2,707,902 + Pf6N2E2_2288 0.86 -0.7 2,707,902 + Pf6N2E2_2288 0.86 -0.7 2,707,903 - Pf6N2E2_2288 0.87 -1.3 2,707,903 - Pf6N2E2_2288 0.87 -1.3 2,707,903 - Pf6N2E2_2288 0.87 -2.1 2,707,903 - Pf6N2E2_2288 0.87 +0.3 2,707,911 + -0.4 2,707,925 + +0.3 2,707,925 + +0.3 2,707,926 - +0.3 2,707,982 - -0.7 2,707,984 + -1.3 2,707,985 - -1.4 2,707,985 - -0.8 2,708,043 + -0.7 2,708,044 - +0.3 2,708,044 - -2.7 2,708,362 - Pf6N2E2_2289 0.28 -1.3 2,708,791 - Pf6N2E2_2289 0.65 +0.3
Or see this region's nucleotide sequence