Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2228 and Pf6N2E2_2229 overlap by 4 nucleotides Pf6N2E2_2229 and Pf6N2E2_2230 are separated by 306 nucleotides
Pf6N2E2_2228 - Transcriptional regulator, LysR family, at 2,640,349 to 2,641,245
_2228
Pf6N2E2_2229 - Major facilitator family transporter, at 2,641,242 to 2,642,393
_2229
Pf6N2E2_2230 - Sodium:dicarboxylate symporter, at 2,642,700 to 2,643,908
_2230
Position (kb)
2641
2642
2643 Strain fitness (log2 ratio)
-2
-1
0
1 at 2640.274 kb on + strand at 2640.274 kb on + strand at 2640.274 kb on + strand at 2640.274 kb on + strand at 2640.275 kb on - strand at 2640.275 kb on - strand at 2640.275 kb on - strand at 2640.373 kb on + strand at 2640.374 kb on - strand at 2640.374 kb on - strand at 2640.374 kb on - strand at 2640.390 kb on + strand at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.480 kb on + strand, within Pf6N2E2_2228 at 2640.481 kb on - strand, within Pf6N2E2_2228 at 2640.890 kb on - strand, within Pf6N2E2_2228 at 2641.006 kb on - strand, within Pf6N2E2_2228 at 2641.118 kb on + strand, within Pf6N2E2_2228 at 2641.118 kb on + strand, within Pf6N2E2_2228 at 2641.118 kb on + strand, within Pf6N2E2_2228 at 2641.119 kb on - strand, within Pf6N2E2_2228 at 2641.160 kb on - strand at 2641.160 kb on - strand at 2641.169 kb on - strand at 2641.243 kb on + strand at 2641.243 kb on + strand at 2641.244 kb on - strand at 2641.347 kb on - strand at 2641.644 kb on + strand, within Pf6N2E2_2229 at 2641.729 kb on + strand, within Pf6N2E2_2229 at 2641.729 kb on + strand, within Pf6N2E2_2229 at 2641.730 kb on - strand, within Pf6N2E2_2229 at 2641.736 kb on - strand, within Pf6N2E2_2229 at 2641.736 kb on - strand, within Pf6N2E2_2229 at 2641.736 kb on - strand, within Pf6N2E2_2229 at 2641.865 kb on - strand, within Pf6N2E2_2229 at 2641.865 kb on - strand, within Pf6N2E2_2229 at 2641.865 kb on - strand, within Pf6N2E2_2229 at 2641.868 kb on + strand, within Pf6N2E2_2229 at 2641.868 kb on + strand, within Pf6N2E2_2229 at 2641.869 kb on - strand, within Pf6N2E2_2229 at 2641.869 kb on - strand, within Pf6N2E2_2229 at 2642.180 kb on + strand, within Pf6N2E2_2229 at 2642.180 kb on + strand, within Pf6N2E2_2229 at 2642.268 kb on + strand, within Pf6N2E2_2229 at 2642.354 kb on + strand at 2642.448 kb on + strand at 2642.472 kb on + strand at 2642.473 kb on - strand at 2642.657 kb on + strand at 2642.658 kb on - strand at 2642.700 kb on - strand at 2642.760 kb on - strand at 2642.857 kb on + strand, within Pf6N2E2_2230 at 2642.934 kb on + strand, within Pf6N2E2_2230 at 2642.935 kb on - strand, within Pf6N2E2_2230 at 2642.982 kb on - strand, within Pf6N2E2_2230 at 2643.114 kb on - strand, within Pf6N2E2_2230 at 2643.307 kb on + strand, within Pf6N2E2_2230 at 2643.316 kb on - strand, within Pf6N2E2_2230 at 2643.335 kb on - strand, within Pf6N2E2_2230
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 2,640,274 + +0.3 2,640,274 + -1.7 2,640,274 + +0.3 2,640,274 + +0.3 2,640,275 - -1.3 2,640,275 - -0.4 2,640,275 - -0.7 2,640,373 + -1.3 2,640,374 - +0.3 2,640,374 - +0.3 2,640,374 - +0.3 2,640,390 + +0.3 2,640,478 + Pf6N2E2_2228 0.14 -0.8 2,640,478 + Pf6N2E2_2228 0.14 -1.3 2,640,478 + Pf6N2E2_2228 0.14 +0.3 2,640,478 + Pf6N2E2_2228 0.14 -1.3 2,640,479 - Pf6N2E2_2228 0.14 -0.7 2,640,479 - Pf6N2E2_2228 0.14 -1.3 2,640,479 - Pf6N2E2_2228 0.14 +0.3 2,640,479 - Pf6N2E2_2228 0.14 +0.3 2,640,479 - Pf6N2E2_2228 0.14 -0.7 2,640,480 + Pf6N2E2_2228 0.15 +0.3 2,640,481 - Pf6N2E2_2228 0.15 -0.4 2,640,890 - Pf6N2E2_2228 0.60 +0.3 2,641,006 - Pf6N2E2_2228 0.73 +0.3 2,641,118 + Pf6N2E2_2228 0.86 -0.7 2,641,118 + Pf6N2E2_2228 0.86 +0.3 2,641,118 + Pf6N2E2_2228 0.86 -0.2 2,641,119 - Pf6N2E2_2228 0.86 +0.3 2,641,160 - +0.3 2,641,160 - -0.7 2,641,169 - -1.5 2,641,243 + -2.1 2,641,243 + -0.7 2,641,244 - +0.3 2,641,347 - -1.7 2,641,644 + Pf6N2E2_2229 0.35 -0.7 2,641,729 + Pf6N2E2_2229 0.42 +0.3 2,641,729 + Pf6N2E2_2229 0.42 -1.3 2,641,730 - Pf6N2E2_2229 0.42 -0.7 2,641,736 - Pf6N2E2_2229 0.43 -0.7 2,641,736 - Pf6N2E2_2229 0.43 +0.3 2,641,736 - Pf6N2E2_2229 0.43 -1.4 2,641,865 - Pf6N2E2_2229 0.54 +0.3 2,641,865 - Pf6N2E2_2229 0.54 -0.2 2,641,865 - Pf6N2E2_2229 0.54 +0.3 2,641,868 + Pf6N2E2_2229 0.54 -2.1 2,641,868 + Pf6N2E2_2229 0.54 -0.7 2,641,869 - Pf6N2E2_2229 0.54 +0.3 2,641,869 - Pf6N2E2_2229 0.54 -0.7 2,642,180 + Pf6N2E2_2229 0.81 -1.3 2,642,180 + Pf6N2E2_2229 0.81 +0.3 2,642,268 + Pf6N2E2_2229 0.89 -1.7 2,642,354 + -0.4 2,642,448 + -0.6 2,642,472 + -0.7 2,642,473 - -1.3 2,642,657 + +0.3 2,642,658 - +0.3 2,642,700 - +0.3 2,642,760 - -0.7 2,642,857 + Pf6N2E2_2230 0.13 -0.7 2,642,934 + Pf6N2E2_2230 0.19 -1.4 2,642,935 - Pf6N2E2_2230 0.19 -0.5 2,642,982 - Pf6N2E2_2230 0.23 -0.3 2,643,114 - Pf6N2E2_2230 0.34 -0.1 2,643,307 + Pf6N2E2_2230 0.50 +0.3 2,643,316 - Pf6N2E2_2230 0.51 +0.3 2,643,335 - Pf6N2E2_2230 0.53 -1.3
Or see this region's nucleotide sequence