Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_164 and Pf6N2E2_165 are separated by 23 nucleotides Pf6N2E2_165 and Pf6N2E2_166 overlap by 4 nucleotides Pf6N2E2_166 and Pf6N2E2_167 are separated by 30 nucleotides
Pf6N2E2_164 - Ribose operon repressor, at 217,827 to 218,846
_164
Pf6N2E2_165 - Ribokinase (EC 2.7.1.15), at 218,870 to 219,784
_165
Pf6N2E2_166 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 219,781 to 220,185
_166
Pf6N2E2_167 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 220,216 to 221,244
_167
Position (kb) 218
219
220 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 217.959 kb on + strand, within Pf6N2E2_164 at 217.959 kb on + strand, within Pf6N2E2_164 at 217.960 kb on - strand, within Pf6N2E2_164 at 218.043 kb on + strand, within Pf6N2E2_164 at 218.044 kb on - strand, within Pf6N2E2_164 at 218.044 kb on - strand, within Pf6N2E2_164 at 218.076 kb on + strand, within Pf6N2E2_164 at 218.076 kb on + strand, within Pf6N2E2_164 at 218.076 kb on + strand, within Pf6N2E2_164 at 218.145 kb on + strand, within Pf6N2E2_164 at 218.145 kb on + strand, within Pf6N2E2_164 at 218.291 kb on - strand, within Pf6N2E2_164 at 218.310 kb on + strand, within Pf6N2E2_164 at 218.310 kb on + strand, within Pf6N2E2_164 at 218.311 kb on - strand, within Pf6N2E2_164 at 218.319 kb on + strand, within Pf6N2E2_164 at 218.320 kb on - strand, within Pf6N2E2_164 at 218.387 kb on + strand, within Pf6N2E2_164 at 218.524 kb on + strand, within Pf6N2E2_164 at 218.524 kb on + strand, within Pf6N2E2_164 at 218.525 kb on - strand, within Pf6N2E2_164 at 218.525 kb on - strand, within Pf6N2E2_164 at 218.662 kb on - strand, within Pf6N2E2_164 at 218.662 kb on - strand, within Pf6N2E2_164 at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.790 kb on - strand at 218.790 kb on - strand at 218.790 kb on - strand at 218.861 kb on + strand at 218.861 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.883 kb on + strand at 218.883 kb on + strand at 218.883 kb on + strand at 218.883 kb on + strand at 218.884 kb on - strand at 218.890 kb on - strand at 218.892 kb on + strand at 218.892 kb on + strand at 218.892 kb on + strand at 219.377 kb on - strand, within Pf6N2E2_165 at 219.395 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.765 kb on + strand at 219.766 kb on - strand at 219.766 kb on - strand at 219.766 kb on - strand at 220.184 kb on - strand at 220.184 kb on - strand at 220.184 kb on - strand at 220.226 kb on - strand at 220.393 kb on - strand, within Pf6N2E2_167 at 220.393 kb on - strand, within Pf6N2E2_167 at 220.393 kb on - strand, within Pf6N2E2_167 at 220.393 kb on - strand, within Pf6N2E2_167 at 220.495 kb on + strand, within Pf6N2E2_167 at 220.496 kb on - strand, within Pf6N2E2_167 at 220.580 kb on + strand, within Pf6N2E2_167 at 220.581 kb on - strand, within Pf6N2E2_167 at 220.581 kb on - strand, within Pf6N2E2_167 at 220.624 kb on + strand, within Pf6N2E2_167
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 217,959 + Pf6N2E2_164 0.13 +0.3 217,959 + Pf6N2E2_164 0.13 -3.4 217,960 - Pf6N2E2_164 0.13 -1.3 218,043 + Pf6N2E2_164 0.21 +0.3 218,044 - Pf6N2E2_164 0.21 +0.3 218,044 - Pf6N2E2_164 0.21 -0.7 218,076 + Pf6N2E2_164 0.24 +0.3 218,076 + Pf6N2E2_164 0.24 -1.4 218,076 + Pf6N2E2_164 0.24 -0.7 218,145 + Pf6N2E2_164 0.31 -0.7 218,145 + Pf6N2E2_164 0.31 +0.3 218,291 - Pf6N2E2_164 0.45 -6.9 218,310 + Pf6N2E2_164 0.47 -0.7 218,310 + Pf6N2E2_164 0.47 -0.7 218,311 - Pf6N2E2_164 0.47 +0.3 218,319 + Pf6N2E2_164 0.48 -1.3 218,320 - Pf6N2E2_164 0.48 -2.3 218,387 + Pf6N2E2_164 0.55 -3.2 218,524 + Pf6N2E2_164 0.68 -1.3 218,524 + Pf6N2E2_164 0.68 -0.7 218,525 - Pf6N2E2_164 0.68 -1.3 218,525 - Pf6N2E2_164 0.68 +0.3 218,662 - Pf6N2E2_164 0.82 -2.3 218,662 - Pf6N2E2_164 0.82 -3.4 218,789 + +0.3 218,789 + +0.3 218,789 + +0.3 218,789 + +0.3 218,789 + -0.7 218,789 + +2.6 218,789 + +0.3 218,789 + +1.5 218,789 + +0.3 218,789 + +1.3 218,790 - +0.3 218,790 - -3.7 218,790 - -3.9 218,861 + -0.7 218,861 + +0.3 218,869 + +0.3 218,869 + +0.3 218,869 + +0.3 218,869 + -3.3 218,869 + +0.3 218,869 + +0.3 218,869 + +0.3 218,869 + +0.3 218,869 + -1.0 218,869 + -0.7 218,870 - -0.7 218,870 - +0.3 218,870 - -2.9 218,870 - +0.3 218,870 - +0.3 218,870 - +0.3 218,870 - -0.7 218,870 - -1.3 218,883 + -2.9 218,883 + -1.3 218,883 + -2.0 218,883 + -3.6 218,884 - +0.3 218,890 - -3.3 218,892 + -3.1 218,892 + +0.3 218,892 + -3.8 219,377 - Pf6N2E2_165 0.55 +0.3 219,395 + Pf6N2E2_165 0.57 +0.3 219,421 + Pf6N2E2_165 0.60 -0.7 219,421 + Pf6N2E2_165 0.60 -0.7 219,421 + Pf6N2E2_165 0.60 +0.3 219,421 + Pf6N2E2_165 0.60 -1.7 219,765 + -0.8 219,766 - -3.5 219,766 - +0.3 219,766 - -0.3 220,184 - +0.3 220,184 - +0.3 220,184 - +0.3 220,226 - +0.3 220,393 - Pf6N2E2_167 0.17 -0.9 220,393 - Pf6N2E2_167 0.17 -0.7 220,393 - Pf6N2E2_167 0.17 +0.3 220,393 - Pf6N2E2_167 0.17 +0.3 220,495 + Pf6N2E2_167 0.27 +0.5 220,496 - Pf6N2E2_167 0.27 +0.3 220,580 + Pf6N2E2_167 0.35 +0.3 220,581 - Pf6N2E2_167 0.35 +0.3 220,581 - Pf6N2E2_167 0.35 +0.3 220,624 + Pf6N2E2_167 0.40 +0.3
Or see this region's nucleotide sequence