Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1552 and Pf6N2E2_1553 are separated by 78 nucleotides Pf6N2E2_1553 and Pf6N2E2_1554 are separated by 185 nucleotides Pf6N2E2_1554 and Pf6N2E2_1555 are separated by 199 nucleotides
Pf6N2E2_1552 - Transcriptional regulator, LysR family, at 1,811,318 to 1,812,238
_1552
Pf6N2E2_1553 - Glutathione S-transferase, unnamed subgroup (EC 2.5.1.18), at 1,812,317 to 1,812,946
_1553
Pf6N2E2_1554 - Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17), at 1,813,132 to 1,815,186
_1554
Pf6N2E2_1555 - Sensor histidine kinase/response regulator, at 1,815,386 to 1,816,999
_1555
Position (kb)
1813
1814
1815
1816 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1812.158 kb on - strand at 1812.158 kb on - strand at 1812.450 kb on + strand, within Pf6N2E2_1553 at 1812.450 kb on + strand, within Pf6N2E2_1553 at 1812.450 kb on + strand, within Pf6N2E2_1553 at 1812.450 kb on + strand, within Pf6N2E2_1553 at 1812.450 kb on + strand, within Pf6N2E2_1553 at 1812.450 kb on + strand, within Pf6N2E2_1553 at 1812.450 kb on + strand, within Pf6N2E2_1553 at 1812.451 kb on - strand, within Pf6N2E2_1553 at 1812.451 kb on - strand, within Pf6N2E2_1553 at 1812.451 kb on - strand, within Pf6N2E2_1553 at 1812.451 kb on - strand, within Pf6N2E2_1553 at 1812.451 kb on - strand, within Pf6N2E2_1553 at 1812.451 kb on - strand at 1812.706 kb on - strand, within Pf6N2E2_1553 at 1812.706 kb on - strand, within Pf6N2E2_1553 at 1812.845 kb on + strand, within Pf6N2E2_1553 at 1812.951 kb on + strand at 1812.951 kb on + strand at 1812.951 kb on + strand at 1812.952 kb on - strand at 1812.952 kb on - strand at 1812.952 kb on - strand at 1812.952 kb on - strand at 1812.952 kb on - strand at 1812.952 kb on - strand at 1812.952 kb on - strand at 1812.998 kb on + strand at 1813.039 kb on + strand at 1813.039 kb on + strand at 1813.040 kb on - strand at 1813.040 kb on - strand at 1813.040 kb on - strand at 1813.040 kb on - strand at 1813.040 kb on - strand at 1813.084 kb on - strand at 1813.093 kb on + strand at 1813.100 kb on - strand at 1813.536 kb on + strand, within Pf6N2E2_1554 at 1813.559 kb on + strand, within Pf6N2E2_1554 at 1813.559 kb on + strand, within Pf6N2E2_1554 at 1813.560 kb on - strand, within Pf6N2E2_1554 at 1813.560 kb on - strand, within Pf6N2E2_1554 at 1813.628 kb on + strand, within Pf6N2E2_1554 at 1813.628 kb on + strand, within Pf6N2E2_1554 at 1813.629 kb on - strand, within Pf6N2E2_1554 at 1813.684 kb on + strand, within Pf6N2E2_1554 at 1813.684 kb on + strand, within Pf6N2E2_1554 at 1813.685 kb on - strand, within Pf6N2E2_1554 at 1813.806 kb on - strand, within Pf6N2E2_1554 at 1813.806 kb on - strand, within Pf6N2E2_1554 at 1814.030 kb on + strand, within Pf6N2E2_1554 at 1814.030 kb on + strand, within Pf6N2E2_1554 at 1814.031 kb on - strand, within Pf6N2E2_1554 at 1814.616 kb on - strand, within Pf6N2E2_1554 at 1814.616 kb on - strand, within Pf6N2E2_1554 at 1814.616 kb on - strand, within Pf6N2E2_1554 at 1814.616 kb on - strand, within Pf6N2E2_1554 at 1814.616 kb on - strand, within Pf6N2E2_1554 at 1814.616 kb on - strand, within Pf6N2E2_1554 at 1814.616 kb on - strand, within Pf6N2E2_1554 at 1814.616 kb on - strand, within Pf6N2E2_1554 at 1814.616 kb on - strand, within Pf6N2E2_1554 at 1814.627 kb on + strand, within Pf6N2E2_1554 at 1814.628 kb on - strand, within Pf6N2E2_1554 at 1814.738 kb on + strand, within Pf6N2E2_1554 at 1814.739 kb on - strand, within Pf6N2E2_1554 at 1814.756 kb on + strand, within Pf6N2E2_1554 at 1814.803 kb on + strand, within Pf6N2E2_1554 at 1814.803 kb on + strand, within Pf6N2E2_1554 at 1814.804 kb on - strand, within Pf6N2E2_1554 at 1814.804 kb on - strand, within Pf6N2E2_1554 at 1814.804 kb on - strand, within Pf6N2E2_1554 at 1815.039 kb on - strand at 1815.039 kb on - strand at 1815.070 kb on - strand at 1815.192 kb on - strand at 1815.192 kb on - strand at 1815.315 kb on + strand at 1815.316 kb on - strand at 1816.014 kb on + strand, within Pf6N2E2_1555 at 1816.015 kb on - strand, within Pf6N2E2_1555 at 1816.015 kb on - strand, within Pf6N2E2_1555 at 1816.015 kb on - strand, within Pf6N2E2_1555 at 1816.015 kb on - strand, within Pf6N2E2_1555 at 1816.015 kb on - strand, within Pf6N2E2_1555 at 1816.015 kb on - strand, within Pf6N2E2_1555 at 1816.080 kb on + strand, within Pf6N2E2_1555 at 1816.080 kb on + strand, within Pf6N2E2_1555 at 1816.080 kb on + strand, within Pf6N2E2_1555 at 1816.081 kb on - strand, within Pf6N2E2_1555 at 1816.081 kb on - strand, within Pf6N2E2_1555 at 1816.081 kb on - strand, within Pf6N2E2_1555
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 1,812,158 - -0.4 1,812,158 - +0.1 1,812,450 + Pf6N2E2_1553 0.21 +0.1 1,812,450 + Pf6N2E2_1553 0.21 +0.1 1,812,450 + Pf6N2E2_1553 0.21 -1.4 1,812,450 + Pf6N2E2_1553 0.21 +0.1 1,812,450 + Pf6N2E2_1553 0.21 +0.1 1,812,450 + Pf6N2E2_1553 0.21 +0.1 1,812,450 + Pf6N2E2_1553 0.21 -0.9 1,812,451 - Pf6N2E2_1553 0.21 +0.1 1,812,451 - Pf6N2E2_1553 0.21 -0.9 1,812,451 - Pf6N2E2_1553 0.21 +0.1 1,812,451 - Pf6N2E2_1553 0.21 +0.1 1,812,451 - Pf6N2E2_1553 0.21 +0.1 1,812,451 - -0.4 1,812,706 - Pf6N2E2_1553 0.62 +0.1 1,812,706 - Pf6N2E2_1553 0.62 +0.1 1,812,845 + Pf6N2E2_1553 0.84 +0.1 1,812,951 + +0.1 1,812,951 + +0.1 1,812,951 + -1.4 1,812,952 - -1.4 1,812,952 - +0.1 1,812,952 - +0.1 1,812,952 - +0.1 1,812,952 - +0.1 1,812,952 - -0.9 1,812,952 - -0.9 1,812,998 + -0.9 1,813,039 + +0.1 1,813,039 + +0.1 1,813,040 - -1.4 1,813,040 - -0.9 1,813,040 - +0.1 1,813,040 - +0.1 1,813,040 - +0.1 1,813,084 - +0.1 1,813,093 + -0.9 1,813,100 - -0.9 1,813,536 + Pf6N2E2_1554 0.20 +0.1 1,813,559 + Pf6N2E2_1554 0.21 -0.9 1,813,559 + Pf6N2E2_1554 0.21 +0.1 1,813,560 - Pf6N2E2_1554 0.21 +0.1 1,813,560 - Pf6N2E2_1554 0.21 +0.1 1,813,628 + Pf6N2E2_1554 0.24 -1.3 1,813,628 + Pf6N2E2_1554 0.24 -2.3 1,813,629 - Pf6N2E2_1554 0.24 +0.1 1,813,684 + Pf6N2E2_1554 0.27 -1.9 1,813,684 + Pf6N2E2_1554 0.27 +0.1 1,813,685 - Pf6N2E2_1554 0.27 +0.1 1,813,806 - Pf6N2E2_1554 0.33 +0.1 1,813,806 - Pf6N2E2_1554 0.33 -0.9 1,814,030 + Pf6N2E2_1554 0.44 +0.1 1,814,030 + Pf6N2E2_1554 0.44 +0.1 1,814,031 - Pf6N2E2_1554 0.44 +0.1 1,814,616 - Pf6N2E2_1554 0.72 +0.1 1,814,616 - Pf6N2E2_1554 0.72 +0.1 1,814,616 - Pf6N2E2_1554 0.72 +0.1 1,814,616 - Pf6N2E2_1554 0.72 -1.9 1,814,616 - Pf6N2E2_1554 0.72 -0.9 1,814,616 - Pf6N2E2_1554 0.72 -0.7 1,814,616 - Pf6N2E2_1554 0.72 +0.1 1,814,616 - Pf6N2E2_1554 0.72 +0.1 1,814,616 - Pf6N2E2_1554 0.72 -1.2 1,814,627 + Pf6N2E2_1554 0.73 -1.4 1,814,628 - Pf6N2E2_1554 0.73 -1.4 1,814,738 + Pf6N2E2_1554 0.78 +0.1 1,814,739 - Pf6N2E2_1554 0.78 +0.1 1,814,756 + Pf6N2E2_1554 0.79 -0.1 1,814,803 + Pf6N2E2_1554 0.81 -6.9 1,814,803 + Pf6N2E2_1554 0.81 -0.1 1,814,804 - Pf6N2E2_1554 0.81 +0.1 1,814,804 - Pf6N2E2_1554 0.81 +0.1 1,814,804 - Pf6N2E2_1554 0.81 +0.1 1,815,039 - +0.1 1,815,039 - -0.3 1,815,070 - -0.9 1,815,192 - -1.9 1,815,192 - +0.1 1,815,315 + -1.2 1,815,316 - +0.1 1,816,014 + Pf6N2E2_1555 0.39 -1.9 1,816,015 - Pf6N2E2_1555 0.39 +0.1 1,816,015 - Pf6N2E2_1555 0.39 -1.4 1,816,015 - Pf6N2E2_1555 0.39 -0.9 1,816,015 - Pf6N2E2_1555 0.39 -0.9 1,816,015 - Pf6N2E2_1555 0.39 +0.1 1,816,015 - Pf6N2E2_1555 0.39 -0.3 1,816,080 + Pf6N2E2_1555 0.43 +0.1 1,816,080 + Pf6N2E2_1555 0.43 +0.1 1,816,080 + Pf6N2E2_1555 0.43 +0.1 1,816,081 - Pf6N2E2_1555 0.43 -2.9 1,816,081 - Pf6N2E2_1555 0.43 +0.1 1,816,081 - Pf6N2E2_1555 0.43 +0.1
Or see this region's nucleotide sequence