Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1497 and Pf6N2E2_1498 overlap by 4 nucleotides Pf6N2E2_1498 and Pf6N2E2_1499 are separated by 52 nucleotides
Pf6N2E2_1497 - D-2-hydroxyglutarate dehydrogenase, at 1,750,690 to 1,752,114
_1497
Pf6N2E2_1498 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 1,752,111 to 1,752,962
_1498
Pf6N2E2_1499 - ABC transporter amino acid-binding protein, at 1,753,015 to 1,753,857
_1499
Position (kb)
1752
1753 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1751.116 kb on - strand, within Pf6N2E2_1497 at 1751.116 kb on - strand, within Pf6N2E2_1497 at 1751.499 kb on + strand, within Pf6N2E2_1497 at 1751.499 kb on + strand, within Pf6N2E2_1497 at 1751.500 kb on - strand, within Pf6N2E2_1497 at 1751.987 kb on - strand at 1752.036 kb on - strand at 1752.146 kb on - strand at 1752.381 kb on + strand, within Pf6N2E2_1498 at 1752.381 kb on + strand, within Pf6N2E2_1498 at 1752.382 kb on - strand, within Pf6N2E2_1498 at 1752.382 kb on - strand, within Pf6N2E2_1498 at 1752.387 kb on + strand, within Pf6N2E2_1498 at 1752.388 kb on - strand, within Pf6N2E2_1498 at 1752.593 kb on - strand, within Pf6N2E2_1498 at 1752.608 kb on - strand, within Pf6N2E2_1498 at 1752.769 kb on + strand, within Pf6N2E2_1498 at 1752.770 kb on - strand, within Pf6N2E2_1498 at 1752.770 kb on - strand, within Pf6N2E2_1498 at 1752.970 kb on + strand at 1752.970 kb on + strand at 1752.971 kb on - strand at 1753.296 kb on + strand, within Pf6N2E2_1499 at 1753.379 kb on + strand, within Pf6N2E2_1499 at 1753.380 kb on - strand, within Pf6N2E2_1499 at 1753.453 kb on + strand, within Pf6N2E2_1499 at 1753.479 kb on - strand, within Pf6N2E2_1499 at 1753.862 kb on - strand at 1753.947 kb on - strand at 1753.957 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 1,751,116 - Pf6N2E2_1497 0.30 +0.1 1,751,116 - Pf6N2E2_1497 0.30 +0.1 1,751,499 + Pf6N2E2_1497 0.57 +0.1 1,751,499 + Pf6N2E2_1497 0.57 -0.9 1,751,500 - Pf6N2E2_1497 0.57 +0.1 1,751,987 - +0.1 1,752,036 - +0.1 1,752,146 - -1.1 1,752,381 + Pf6N2E2_1498 0.32 +0.1 1,752,381 + Pf6N2E2_1498 0.32 +0.1 1,752,382 - Pf6N2E2_1498 0.32 -0.9 1,752,382 - Pf6N2E2_1498 0.32 -1.5 1,752,387 + Pf6N2E2_1498 0.32 -0.9 1,752,388 - Pf6N2E2_1498 0.33 -4.2 1,752,593 - Pf6N2E2_1498 0.57 +0.1 1,752,608 - Pf6N2E2_1498 0.58 -0.9 1,752,769 + Pf6N2E2_1498 0.77 +0.1 1,752,770 - Pf6N2E2_1498 0.77 -0.7 1,752,770 - Pf6N2E2_1498 0.77 +0.1 1,752,970 + +0.1 1,752,970 + +0.1 1,752,971 - -0.9 1,753,296 + Pf6N2E2_1499 0.33 +0.1 1,753,379 + Pf6N2E2_1499 0.43 +0.1 1,753,380 - Pf6N2E2_1499 0.43 +0.1 1,753,453 + Pf6N2E2_1499 0.52 -0.5 1,753,479 - Pf6N2E2_1499 0.55 -0.9 1,753,862 - +0.1 1,753,947 - +0.1 1,753,957 + -0.9
Or see this region's nucleotide sequence