Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_669

Experiment: Ying_Nucleobase14 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_668 and Pf6N2E2_669 are separated by 70 nucleotidesPf6N2E2_669 and Pf6N2E2_670 are separated by 137 nucleotides Pf6N2E2_668 - 2,3-butanediol dehydrogenase, R-alcohol forming, (R)- and (S)-acetoin-specific (EC 1.1.1.4), at 773,226 to 774,320 _668 Pf6N2E2_669 - Nuclease inhibitor, at 774,391 to 774,945 _669 Pf6N2E2_670 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 775,083 to 776,000 _670 Position (kb) 774 775Strain fitness (log2 ratio) -2 -1 0 1at 773.568 kb on + strand, within Pf6N2E2_668at 773.568 kb on + strand, within Pf6N2E2_668at 773.569 kb on - strand, within Pf6N2E2_668at 773.569 kb on - strand, within Pf6N2E2_668at 773.572 kb on - strand, within Pf6N2E2_668at 773.572 kb on - strand, within Pf6N2E2_668at 773.572 kb on - strand, within Pf6N2E2_668at 773.572 kb on - strand, within Pf6N2E2_668at 773.590 kb on - strand, within Pf6N2E2_668at 773.676 kb on + strand, within Pf6N2E2_668at 773.676 kb on + strand, within Pf6N2E2_668at 774.147 kb on + strand, within Pf6N2E2_668at 774.148 kb on - strand, within Pf6N2E2_668at 774.148 kb on - strand, within Pf6N2E2_668at 774.148 kb on - strand, within Pf6N2E2_668at 774.466 kb on - strand, within Pf6N2E2_669at 774.466 kb on - strand, within Pf6N2E2_669at 774.746 kb on - strand, within Pf6N2E2_669at 774.782 kb on + strand, within Pf6N2E2_669at 774.783 kb on - strand, within Pf6N2E2_669at 774.783 kb on - strand, within Pf6N2E2_669at 774.858 kb on - strand, within Pf6N2E2_669at 774.938 kb on + strandat 774.938 kb on + strandat 774.939 kb on - strandat 775.072 kb on + strandat 775.084 kb on + strandat 775.147 kb on + strandat 775.147 kb on + strandat 775.148 kb on - strandat 775.148 kb on - strandat 775.288 kb on + strand, within Pf6N2E2_670at 775.288 kb on + strandat 775.289 kb on - strand, within Pf6N2E2_670at 775.289 kb on - strand, within Pf6N2E2_670at 775.289 kb on - strand, within Pf6N2E2_670at 775.416 kb on + strand, within Pf6N2E2_670at 775.416 kb on + strand, within Pf6N2E2_670at 775.416 kb on + strand, within Pf6N2E2_670at 775.417 kb on - strand, within Pf6N2E2_670at 775.570 kb on + strand, within Pf6N2E2_670at 775.570 kb on + strand, within Pf6N2E2_670at 775.571 kb on - strand, within Pf6N2E2_670at 775.571 kb on - strand, within Pf6N2E2_670at 775.588 kb on + strand, within Pf6N2E2_670at 775.589 kb on - strand, within Pf6N2E2_670at 775.589 kb on - strand, within Pf6N2E2_670at 775.589 kb on - strand, within Pf6N2E2_670at 775.663 kb on + strand, within Pf6N2E2_670at 775.663 kb on + strand, within Pf6N2E2_670

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 3
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773,568 + Pf6N2E2_668 0.31 -0.8
773,568 + Pf6N2E2_668 0.31 -1.2
773,569 - Pf6N2E2_668 0.31 -0.5
773,569 - Pf6N2E2_668 0.31 +0.2
773,572 - Pf6N2E2_668 0.32 +0.2
773,572 - Pf6N2E2_668 0.32 +0.2
773,572 - Pf6N2E2_668 0.32 -0.6
773,572 - Pf6N2E2_668 0.32 -0.8
773,590 - Pf6N2E2_668 0.33 +0.2
773,676 + Pf6N2E2_668 0.41 -0.6
773,676 + Pf6N2E2_668 0.41 +0.2
774,147 + Pf6N2E2_668 0.84 -0.8
774,148 - Pf6N2E2_668 0.84 +0.2
774,148 - Pf6N2E2_668 0.84 +0.2
774,148 - Pf6N2E2_668 0.84 +0.2
774,466 - Pf6N2E2_669 0.14 -0.8
774,466 - Pf6N2E2_669 0.14 +0.2
774,746 - Pf6N2E2_669 0.64 -1.2
774,782 + Pf6N2E2_669 0.70 -0.4
774,783 - Pf6N2E2_669 0.71 +0.2
774,783 - Pf6N2E2_669 0.71 -1.8
774,858 - Pf6N2E2_669 0.84 +0.2
774,938 + +0.2
774,938 + +0.2
774,939 - +0.2
775,072 + -1.2
775,084 + +0.2
775,147 + -0.8
775,147 + +0.2
775,148 - -0.8
775,148 - +0.2
775,288 + Pf6N2E2_670 0.22 +0.2
775,288 + +0.2
775,289 - Pf6N2E2_670 0.22 +0.2
775,289 - Pf6N2E2_670 0.22 +0.2
775,289 - Pf6N2E2_670 0.22 -1.8
775,416 + Pf6N2E2_670 0.36 -0.9
775,416 + Pf6N2E2_670 0.36 +0.2
775,416 + Pf6N2E2_670 0.36 -0.5
775,417 - Pf6N2E2_670 0.36 +0.2
775,570 + Pf6N2E2_670 0.53 -2.1
775,570 + Pf6N2E2_670 0.53 -1.2
775,571 - Pf6N2E2_670 0.53 +0.2
775,571 - Pf6N2E2_670 0.53 -1.4
775,588 + Pf6N2E2_670 0.55 -1.5
775,589 - Pf6N2E2_670 0.55 -0.8
775,589 - Pf6N2E2_670 0.55 +0.2
775,589 - Pf6N2E2_670 0.55 +0.2
775,663 + Pf6N2E2_670 0.63 -0.6
775,663 + Pf6N2E2_670 0.63 -0.8

Or see this region's nucleotide sequence