Experiment: Ying_Nucleobase14 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4499 and Pf6N2E2_4500 overlap by 11 nucleotides Pf6N2E2_4500 and Pf6N2E2_4501 are separated by 438 nucleotides Pf6N2E2_4501 and Pf6N2E2_4502 are separated by 326 nucleotides
Pf6N2E2_4499 - protein of unknown function DUF86, at 5,232,337 to 5,232,765
_4499
Pf6N2E2_4500 - hypothetical protein, at 5,232,755 to 5,232,916
_4500
Pf6N2E2_4501 - Alkyl hydroperoxide reductase subunit C-like protein, at 5,233,355 to 5,234,122
_4501
Pf6N2E2_4502 - FMN reductase (EC 1.5.1.29), at 5,234,449 to 5,235,042
_4502
Position (kb)
5233
5234
5235 Strain fitness (log2 ratio)
-2
-1
0
1 at 5232.602 kb on - strand, within Pf6N2E2_4499 at 5232.679 kb on - strand, within Pf6N2E2_4499 at 5232.696 kb on - strand, within Pf6N2E2_4499 at 5232.696 kb on - strand, within Pf6N2E2_4499 at 5232.752 kb on - strand at 5232.752 kb on - strand at 5232.752 kb on - strand at 5233.131 kb on - strand at 5233.131 kb on - strand at 5233.131 kb on - strand at 5233.134 kb on - strand at 5233.134 kb on - strand at 5233.134 kb on - strand at 5233.158 kb on - strand at 5233.158 kb on - strand at 5233.190 kb on - strand at 5233.805 kb on + strand, within Pf6N2E2_4501 at 5233.805 kb on + strand, within Pf6N2E2_4501 at 5233.806 kb on - strand, within Pf6N2E2_4501 at 5233.806 kb on - strand, within Pf6N2E2_4501 at 5233.823 kb on - strand, within Pf6N2E2_4501 at 5233.895 kb on - strand, within Pf6N2E2_4501 at 5233.895 kb on - strand, within Pf6N2E2_4501 at 5233.898 kb on + strand, within Pf6N2E2_4501 at 5233.970 kb on + strand, within Pf6N2E2_4501 at 5233.970 kb on + strand, within Pf6N2E2_4501 at 5233.970 kb on + strand, within Pf6N2E2_4501 at 5233.970 kb on + strand, within Pf6N2E2_4501 at 5233.970 kb on + strand, within Pf6N2E2_4501 at 5233.970 kb on + strand, within Pf6N2E2_4501 at 5233.971 kb on - strand, within Pf6N2E2_4501 at 5233.971 kb on - strand, within Pf6N2E2_4501 at 5233.971 kb on - strand, within Pf6N2E2_4501 at 5233.971 kb on - strand, within Pf6N2E2_4501 at 5234.090 kb on + strand at 5234.091 kb on - strand at 5234.091 kb on - strand at 5234.255 kb on + strand at 5234.256 kb on - strand at 5234.296 kb on + strand at 5234.296 kb on + strand at 5234.297 kb on - strand at 5234.303 kb on + strand at 5234.303 kb on + strand at 5234.303 kb on + strand at 5234.304 kb on - strand at 5234.304 kb on - strand at 5234.304 kb on - strand at 5234.369 kb on - strand at 5234.382 kb on + strand at 5234.396 kb on + strand at 5234.397 kb on - strand at 5234.397 kb on - strand at 5234.434 kb on + strand at 5234.434 kb on + strand at 5234.434 kb on + strand at 5234.434 kb on + strand at 5234.434 kb on + strand at 5234.441 kb on + strand at 5234.441 kb on + strand at 5234.557 kb on + strand, within Pf6N2E2_4502 at 5234.557 kb on + strand, within Pf6N2E2_4502 at 5234.557 kb on + strand, within Pf6N2E2_4502 at 5234.557 kb on + strand, within Pf6N2E2_4502 at 5234.557 kb on + strand, within Pf6N2E2_4502 at 5234.558 kb on - strand, within Pf6N2E2_4502 at 5234.558 kb on - strand, within Pf6N2E2_4502 at 5234.558 kb on - strand, within Pf6N2E2_4502 at 5234.677 kb on + strand, within Pf6N2E2_4502 at 5234.678 kb on - strand, within Pf6N2E2_4502 at 5234.678 kb on - strand, within Pf6N2E2_4502 at 5234.711 kb on + strand, within Pf6N2E2_4502 at 5234.746 kb on - strand, within Pf6N2E2_4502 at 5234.787 kb on + strand, within Pf6N2E2_4502 at 5234.803 kb on + strand, within Pf6N2E2_4502 at 5234.804 kb on - strand, within Pf6N2E2_4502 at 5235.002 kb on + strand at 5235.069 kb on + strand at 5235.069 kb on + strand at 5235.069 kb on + strand at 5235.069 kb on + strand at 5235.070 kb on - strand at 5235.070 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 3 remove 5,232,602 - Pf6N2E2_4499 0.62 +0.5 5,232,679 - Pf6N2E2_4499 0.80 -1.1 5,232,696 - Pf6N2E2_4499 0.84 +0.5 5,232,696 - Pf6N2E2_4499 0.84 +0.5 5,232,752 - +0.5 5,232,752 - +0.5 5,232,752 - +0.5 5,233,131 - -1.1 5,233,131 - +0.5 5,233,131 - -0.5 5,233,134 - -0.5 5,233,134 - +0.5 5,233,134 - +0.5 5,233,158 - -1.5 5,233,158 - -0.9 5,233,190 - -0.5 5,233,805 + Pf6N2E2_4501 0.59 -1.5 5,233,805 + Pf6N2E2_4501 0.59 -1.1 5,233,806 - Pf6N2E2_4501 0.59 +0.5 5,233,806 - Pf6N2E2_4501 0.59 -0.6 5,233,823 - Pf6N2E2_4501 0.61 +0.9 5,233,895 - Pf6N2E2_4501 0.70 -0.3 5,233,895 - Pf6N2E2_4501 0.70 -0.6 5,233,898 + Pf6N2E2_4501 0.71 -1.0 5,233,970 + Pf6N2E2_4501 0.80 -0.3 5,233,970 + Pf6N2E2_4501 0.80 -0.5 5,233,970 + Pf6N2E2_4501 0.80 -1.0 5,233,970 + Pf6N2E2_4501 0.80 -0.2 5,233,970 + Pf6N2E2_4501 0.80 -0.5 5,233,970 + Pf6N2E2_4501 0.80 +0.5 5,233,971 - Pf6N2E2_4501 0.80 -0.6 5,233,971 - Pf6N2E2_4501 0.80 -0.3 5,233,971 - Pf6N2E2_4501 0.80 +0.5 5,233,971 - Pf6N2E2_4501 0.80 -0.4 5,234,090 + +0.5 5,234,091 - +0.7 5,234,091 - +0.5 5,234,255 + +0.5 5,234,256 - -0.7 5,234,296 + -1.1 5,234,296 + +0.5 5,234,297 - +0.5 5,234,303 + -1.9 5,234,303 + -1.1 5,234,303 + +0.5 5,234,304 - -1.1 5,234,304 - -0.5 5,234,304 - -0.7 5,234,369 - -0.5 5,234,382 + +0.5 5,234,396 + -1.1 5,234,397 - -1.4 5,234,397 - +0.5 5,234,434 + -0.5 5,234,434 + +0.5 5,234,434 + -0.5 5,234,434 + -0.4 5,234,434 + +0.5 5,234,441 + +0.5 5,234,441 + -1.1 5,234,557 + Pf6N2E2_4502 0.18 -0.5 5,234,557 + Pf6N2E2_4502 0.18 -1.9 5,234,557 + Pf6N2E2_4502 0.18 -0.8 5,234,557 + Pf6N2E2_4502 0.18 -1.5 5,234,557 + Pf6N2E2_4502 0.18 -1.5 5,234,558 - Pf6N2E2_4502 0.18 -0.4 5,234,558 - Pf6N2E2_4502 0.18 +0.5 5,234,558 - Pf6N2E2_4502 0.18 -1.1 5,234,677 + Pf6N2E2_4502 0.38 -0.5 5,234,678 - Pf6N2E2_4502 0.39 -0.5 5,234,678 - Pf6N2E2_4502 0.39 -0.5 5,234,711 + Pf6N2E2_4502 0.44 -0.5 5,234,746 - Pf6N2E2_4502 0.50 +0.5 5,234,787 + Pf6N2E2_4502 0.57 -0.5 5,234,803 + Pf6N2E2_4502 0.60 -1.1 5,234,804 - Pf6N2E2_4502 0.60 -0.5 5,235,002 + +0.5 5,235,069 + -0.5 5,235,069 + -0.7 5,235,069 + -1.1 5,235,069 + +0.5 5,235,070 - -0.6 5,235,070 - -1.4
Or see this region's nucleotide sequence