Experiment: Ying_Nucleobase14 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1741 and Pf6N2E2_1742 are separated by 187 nucleotides Pf6N2E2_1742 and Pf6N2E2_1743 overlap by 1 nucleotides
Pf6N2E2_1741 - Transketolase, N-terminal section (EC 2.2.1.1), at 2,053,695 to 2,054,579
_1741
Pf6N2E2_1742 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62), at 2,054,767 to 2,056,158
_1742
Pf6N2E2_1743 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 2,056,158 to 2,057,264
_1743
Position (kb)
2054
2055
2056
2057 Strain fitness (log2 ratio)
-2
-1
0
1 at 2053.792 kb on + strand, within Pf6N2E2_1741 at 2053.793 kb on - strand, within Pf6N2E2_1741 at 2054.107 kb on + strand, within Pf6N2E2_1741 at 2054.107 kb on + strand, within Pf6N2E2_1741 at 2054.118 kb on + strand, within Pf6N2E2_1741 at 2054.119 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.401 kb on + strand, within Pf6N2E2_1741 at 2054.444 kb on - strand, within Pf6N2E2_1741 at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.900 kb on - strand at 2054.900 kb on - strand at 2054.900 kb on - strand at 2054.900 kb on - strand at 2054.926 kb on + strand, within Pf6N2E2_1742 at 2054.926 kb on + strand, within Pf6N2E2_1742 at 2054.927 kb on - strand, within Pf6N2E2_1742 at 2055.341 kb on - strand, within Pf6N2E2_1742 at 2055.678 kb on - strand, within Pf6N2E2_1742 at 2055.728 kb on + strand, within Pf6N2E2_1742 at 2055.729 kb on - strand, within Pf6N2E2_1742 at 2055.750 kb on + strand, within Pf6N2E2_1742 at 2055.805 kb on + strand, within Pf6N2E2_1742 at 2055.942 kb on - strand, within Pf6N2E2_1742 at 2056.227 kb on + strand at 2056.228 kb on - strand at 2056.444 kb on - strand, within Pf6N2E2_1743 at 2056.714 kb on - strand, within Pf6N2E2_1743 at 2056.758 kb on + strand, within Pf6N2E2_1743
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 3 remove 2,053,792 + Pf6N2E2_1741 0.11 +0.2 2,053,793 - Pf6N2E2_1741 0.11 +0.2 2,054,107 + Pf6N2E2_1741 0.47 -0.8 2,054,107 + Pf6N2E2_1741 0.47 -0.8 2,054,118 + Pf6N2E2_1741 0.48 -0.8 2,054,119 - Pf6N2E2_1741 0.48 -0.8 2,054,399 - Pf6N2E2_1741 0.80 +0.2 2,054,399 - Pf6N2E2_1741 0.80 -0.6 2,054,399 - Pf6N2E2_1741 0.80 -0.6 2,054,401 + Pf6N2E2_1741 0.80 -0.8 2,054,444 - Pf6N2E2_1741 0.85 -1.8 2,054,899 + -0.8 2,054,899 + +0.2 2,054,899 + -1.4 2,054,899 + +0.2 2,054,899 + +0.2 2,054,900 - -0.8 2,054,900 - +0.2 2,054,900 - -0.8 2,054,900 - +0.2 2,054,926 + Pf6N2E2_1742 0.11 +0.2 2,054,926 + Pf6N2E2_1742 0.11 +0.2 2,054,927 - Pf6N2E2_1742 0.11 +0.2 2,055,341 - Pf6N2E2_1742 0.41 -0.2 2,055,678 - Pf6N2E2_1742 0.65 +0.2 2,055,728 + Pf6N2E2_1742 0.69 +0.2 2,055,729 - Pf6N2E2_1742 0.69 +0.5 2,055,750 + Pf6N2E2_1742 0.71 +1.2 2,055,805 + Pf6N2E2_1742 0.75 -0.8 2,055,942 - Pf6N2E2_1742 0.84 +0.2 2,056,227 + +0.2 2,056,228 - +0.2 2,056,444 - Pf6N2E2_1743 0.26 -0.7 2,056,714 - Pf6N2E2_1743 0.50 +0.2 2,056,758 + Pf6N2E2_1743 0.54 +1.2
Or see this region's nucleotide sequence