Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_651

Experiment: Ying_OrganicAcid7 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_649 and Pf6N2E2_650 are separated by 16 nucleotidesPf6N2E2_650 and Pf6N2E2_651 overlap by 4 nucleotidesPf6N2E2_651 and Pf6N2E2_652 are separated by 172 nucleotides Pf6N2E2_649 - General secretion pathway protein L, at 737,663 to 738,835 _649 Pf6N2E2_650 - General secretion pathway protein M, at 738,852 to 739,400 _650 Pf6N2E2_651 - General secretion pathway protein D, at 739,397 to 741,760 _651 Pf6N2E2_652 - General secretion pathway protein E, at 741,933 to 743,351 _652 Position (kb) 739 740 741 742Strain fitness (log2 ratio) -2 -1 0 1at 738.413 kb on + strand, within Pf6N2E2_649at 738.834 kb on - strandat 738.834 kb on - strandat 739.008 kb on + strand, within Pf6N2E2_650at 739.152 kb on + strand, within Pf6N2E2_650at 739.152 kb on + strand, within Pf6N2E2_650at 739.153 kb on - strand, within Pf6N2E2_650at 739.153 kb on - strand, within Pf6N2E2_650at 739.153 kb on - strand, within Pf6N2E2_650at 739.156 kb on + strand, within Pf6N2E2_650at 739.157 kb on - strand, within Pf6N2E2_650at 739.597 kb on + strandat 739.946 kb on + strand, within Pf6N2E2_651at 739.947 kb on - strand, within Pf6N2E2_651at 740.004 kb on - strand, within Pf6N2E2_651at 740.354 kb on + strand, within Pf6N2E2_651at 740.354 kb on + strand, within Pf6N2E2_651at 740.354 kb on + strand, within Pf6N2E2_651at 740.402 kb on + strand, within Pf6N2E2_651at 740.522 kb on - strand, within Pf6N2E2_651at 741.155 kb on + strand, within Pf6N2E2_651at 741.155 kb on + strand, within Pf6N2E2_651at 741.155 kb on + strand, within Pf6N2E2_651at 741.156 kb on - strand, within Pf6N2E2_651at 741.156 kb on - strand, within Pf6N2E2_651at 741.156 kb on - strand, within Pf6N2E2_651at 741.156 kb on - strand, within Pf6N2E2_651at 741.278 kb on + strand, within Pf6N2E2_651at 741.278 kb on + strand, within Pf6N2E2_651at 741.278 kb on + strand, within Pf6N2E2_651at 741.278 kb on + strand, within Pf6N2E2_651at 741.279 kb on - strand, within Pf6N2E2_651at 741.530 kb on + strandat 741.530 kb on + strandat 741.531 kb on - strandat 741.531 kb on - strandat 741.531 kb on - strandat 741.535 kb on + strandat 741.565 kb on + strandat 741.622 kb on + strandat 741.823 kb on + strandat 741.824 kb on - strandat 741.917 kb on + strandat 741.948 kb on + strandat 741.948 kb on + strandat 741.948 kb on + strandat 741.976 kb on + strandat 742.122 kb on + strand, within Pf6N2E2_652at 742.123 kb on - strand, within Pf6N2E2_652at 742.701 kb on + strand, within Pf6N2E2_652at 742.701 kb on + strand, within Pf6N2E2_652at 742.701 kb on + strand, within Pf6N2E2_652at 742.702 kb on - strand, within Pf6N2E2_652

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3
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738,413 + Pf6N2E2_649 0.64 -0.9
738,834 - -0.9
738,834 - +0.1
739,008 + Pf6N2E2_650 0.28 -0.9
739,152 + Pf6N2E2_650 0.55 +0.1
739,152 + Pf6N2E2_650 0.55 -0.9
739,153 - Pf6N2E2_650 0.55 +0.1
739,153 - Pf6N2E2_650 0.55 -0.9
739,153 - Pf6N2E2_650 0.55 -0.9
739,156 + Pf6N2E2_650 0.55 -2.2
739,157 - Pf6N2E2_650 0.56 -0.9
739,597 + +0.1
739,946 + Pf6N2E2_651 0.23 +0.1
739,947 - Pf6N2E2_651 0.23 +0.1
740,004 - Pf6N2E2_651 0.26 -1.1
740,354 + Pf6N2E2_651 0.40 +0.1
740,354 + Pf6N2E2_651 0.40 +0.1
740,354 + Pf6N2E2_651 0.40 -1.4
740,402 + Pf6N2E2_651 0.43 +0.1
740,522 - Pf6N2E2_651 0.48 -1.4
741,155 + Pf6N2E2_651 0.74 -1.4
741,155 + Pf6N2E2_651 0.74 +0.1
741,155 + Pf6N2E2_651 0.74 -0.9
741,156 - Pf6N2E2_651 0.74 -0.9
741,156 - Pf6N2E2_651 0.74 +0.1
741,156 - Pf6N2E2_651 0.74 -0.9
741,156 - Pf6N2E2_651 0.74 +0.1
741,278 + Pf6N2E2_651 0.80 -0.9
741,278 + Pf6N2E2_651 0.80 -0.9
741,278 + Pf6N2E2_651 0.80 +0.1
741,278 + Pf6N2E2_651 0.80 +0.1
741,279 - Pf6N2E2_651 0.80 +0.1
741,530 + -0.2
741,530 + -0.4
741,531 - -0.4
741,531 - +0.1
741,531 - +0.1
741,535 + -1.4
741,565 + -0.9
741,622 + +0.1
741,823 + -1.4
741,824 - +0.1
741,917 + +0.1
741,948 + +0.1
741,948 + +0.1
741,948 + -1.9
741,976 + -1.9
742,122 + Pf6N2E2_652 0.13 +0.1
742,123 - Pf6N2E2_652 0.13 +0.1
742,701 + Pf6N2E2_652 0.54 -0.4
742,701 + Pf6N2E2_652 0.54 +0.1
742,701 + Pf6N2E2_652 0.54 +0.1
742,702 - Pf6N2E2_652 0.54 +0.1

Or see this region's nucleotide sequence