Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_489

Experiment: Ying_OrganicAcid7 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_488 and Pf6N2E2_489 are separated by 42 nucleotidesPf6N2E2_489 and Pf6N2E2_490 are separated by 173 nucleotides Pf6N2E2_488 - Gluconolactonase (EC 3.1.1.17), at 554,449 to 555,354 _488 Pf6N2E2_489 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 555,397 to 556,218 _489 Pf6N2E2_490 - PhaK-like protein, at 556,392 to 557,693 _490 Position (kb) 555 556 557Strain fitness (log2 ratio) -1 0 1at 554.711 kb on + strand, within Pf6N2E2_488at 554.712 kb on - strand, within Pf6N2E2_488at 554.814 kb on - strand, within Pf6N2E2_488at 554.861 kb on + strand, within Pf6N2E2_488at 554.861 kb on + strand, within Pf6N2E2_488at 554.861 kb on + strand, within Pf6N2E2_488at 554.861 kb on + strand, within Pf6N2E2_488at 554.861 kb on + strand, within Pf6N2E2_488at 554.861 kb on + strand, within Pf6N2E2_488at 554.862 kb on - strand, within Pf6N2E2_488at 554.960 kb on + strand, within Pf6N2E2_488at 554.961 kb on - strand, within Pf6N2E2_488at 554.961 kb on - strand, within Pf6N2E2_488at 554.961 kb on - strand, within Pf6N2E2_488at 555.231 kb on + strand, within Pf6N2E2_488at 555.231 kb on + strand, within Pf6N2E2_488at 555.276 kb on - strandat 555.440 kb on + strandat 555.584 kb on + strand, within Pf6N2E2_489at 555.584 kb on + strand, within Pf6N2E2_489at 555.615 kb on - strand, within Pf6N2E2_489at 555.747 kb on - strand, within Pf6N2E2_489at 555.770 kb on + strand, within Pf6N2E2_489at 555.770 kb on + strand, within Pf6N2E2_489at 555.771 kb on - strand, within Pf6N2E2_489at 555.771 kb on - strand, within Pf6N2E2_489at 555.791 kb on + strand, within Pf6N2E2_489at 555.791 kb on + strand, within Pf6N2E2_489at 555.791 kb on + strand, within Pf6N2E2_489at 555.843 kb on - strand, within Pf6N2E2_489at 555.911 kb on + strand, within Pf6N2E2_489at 555.911 kb on + strand, within Pf6N2E2_489at 555.911 kb on + strand, within Pf6N2E2_489at 555.911 kb on + strand, within Pf6N2E2_489at 556.177 kb on + strandat 556.177 kb on + strandat 556.177 kb on + strandat 556.178 kb on - strandat 556.178 kb on - strandat 556.178 kb on - strandat 556.224 kb on + strandat 556.225 kb on - strandat 556.237 kb on + strandat 556.237 kb on + strandat 556.246 kb on + strandat 556.246 kb on + strandat 556.300 kb on + strandat 556.393 kb on + strandat 556.453 kb on + strandat 556.453 kb on + strandat 556.516 kb on - strandat 557.014 kb on + strand, within Pf6N2E2_490at 557.014 kb on + strand, within Pf6N2E2_490

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3
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554,711 + Pf6N2E2_488 0.29 +0.2
554,712 - Pf6N2E2_488 0.29 +0.2
554,814 - Pf6N2E2_488 0.40 -1.4
554,861 + Pf6N2E2_488 0.45 +0.2
554,861 + Pf6N2E2_488 0.45 -0.9
554,861 + Pf6N2E2_488 0.45 +0.2
554,861 + Pf6N2E2_488 0.45 -0.8
554,861 + Pf6N2E2_488 0.45 +0.2
554,861 + Pf6N2E2_488 0.45 +0.6
554,862 - Pf6N2E2_488 0.46 +0.2
554,960 + Pf6N2E2_488 0.56 -0.8
554,961 - Pf6N2E2_488 0.57 +0.2
554,961 - Pf6N2E2_488 0.57 -0.8
554,961 - Pf6N2E2_488 0.57 -1.3
555,231 + Pf6N2E2_488 0.86 -0.5
555,231 + Pf6N2E2_488 0.86 +0.2
555,276 - +0.2
555,440 + -0.8
555,584 + Pf6N2E2_489 0.23 +0.2
555,584 + Pf6N2E2_489 0.23 +0.2
555,615 - Pf6N2E2_489 0.27 +0.2
555,747 - Pf6N2E2_489 0.43 -1.4
555,770 + Pf6N2E2_489 0.45 +0.2
555,770 + Pf6N2E2_489 0.45 -0.8
555,771 - Pf6N2E2_489 0.45 -0.8
555,771 - Pf6N2E2_489 0.45 -0.8
555,791 + Pf6N2E2_489 0.48 +0.2
555,791 + Pf6N2E2_489 0.48 +0.2
555,791 + Pf6N2E2_489 0.48 -0.8
555,843 - Pf6N2E2_489 0.54 -1.4
555,911 + Pf6N2E2_489 0.63 -0.8
555,911 + Pf6N2E2_489 0.63 +0.2
555,911 + Pf6N2E2_489 0.63 +0.2
555,911 + Pf6N2E2_489 0.63 +0.2
556,177 + +0.2
556,177 + +0.2
556,177 + -0.6
556,178 - +0.2
556,178 - -1.4
556,178 - -0.6
556,224 + -1.4
556,225 - -0.8
556,237 + +0.2
556,237 + -0.8
556,246 + +0.2
556,246 + +0.2
556,300 + -1.4
556,393 + +0.2
556,453 + +0.2
556,453 + +0.2
556,516 - +0.2
557,014 + Pf6N2E2_490 0.48 +0.2
557,014 + Pf6N2E2_490 0.48 -0.8

Or see this region's nucleotide sequence