Experiment: Ying_OrganicAcid7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3092 and Pf6N2E2_3093 are separated by 93 nucleotides Pf6N2E2_3093 and Pf6N2E2_3094 are separated by 71 nucleotides Pf6N2E2_3094 and Pf6N2E2_3095 overlap by 4 nucleotides Pf6N2E2_3095 and Pf6N2E2_3096 overlap by 22 nucleotides
Pf6N2E2_3092 - RND efflux membrane fusion protein, at 3,580,727 to 3,581,827
_3092
Pf6N2E2_3093 - Transcriptional regulator, TetR family, at 3,581,921 to 3,582,556
_3093
Pf6N2E2_3094 - SAM-dependent methyltransferase (EC 2.1.1.-), at 3,582,628 to 3,583,410
_3094
Pf6N2E2_3095 - hypothetical protein, at 3,583,407 to 3,583,523
_3095
Pf6N2E2_3096 - Periplasmic protein TonB, links inner and outer membranes, at 3,583,502 to 3,584,122
_3096
Position (kb)
3581
3582
3583 Strain fitness (log2 ratio)
-2
-1
0
1 at 3580.932 kb on + strand, within Pf6N2E2_3092 at 3580.932 kb on + strand, within Pf6N2E2_3092 at 3580.932 kb on + strand, within Pf6N2E2_3092 at 3580.932 kb on + strand, within Pf6N2E2_3092 at 3580.932 kb on + strand, within Pf6N2E2_3092 at 3580.932 kb on + strand, within Pf6N2E2_3092 at 3580.932 kb on + strand, within Pf6N2E2_3092 at 3580.932 kb on + strand, within Pf6N2E2_3092 at 3580.932 kb on + strand, within Pf6N2E2_3092 at 3580.933 kb on - strand, within Pf6N2E2_3092 at 3580.933 kb on - strand, within Pf6N2E2_3092 at 3580.933 kb on - strand, within Pf6N2E2_3092 at 3580.933 kb on - strand, within Pf6N2E2_3092 at 3580.933 kb on - strand, within Pf6N2E2_3092 at 3580.933 kb on - strand, within Pf6N2E2_3092 at 3580.933 kb on - strand, within Pf6N2E2_3092 at 3580.933 kb on - strand, within Pf6N2E2_3092 at 3581.332 kb on - strand, within Pf6N2E2_3092 at 3581.464 kb on - strand, within Pf6N2E2_3092 at 3581.682 kb on + strand, within Pf6N2E2_3092 at 3581.777 kb on + strand at 3581.818 kb on - strand at 3581.861 kb on - strand at 3581.861 kb on - strand at 3581.867 kb on - strand at 3581.877 kb on - strand at 3581.877 kb on - strand at 3581.881 kb on + strand at 3581.881 kb on + strand at 3581.885 kb on - strand at 3581.911 kb on - strand at 3582.035 kb on - strand, within Pf6N2E2_3093 at 3582.086 kb on + strand, within Pf6N2E2_3093 at 3582.086 kb on + strand, within Pf6N2E2_3093 at 3582.087 kb on - strand, within Pf6N2E2_3093 at 3582.087 kb on - strand, within Pf6N2E2_3093 at 3582.087 kb on - strand, within Pf6N2E2_3093 at 3582.400 kb on + strand, within Pf6N2E2_3093 at 3582.400 kb on + strand, within Pf6N2E2_3093 at 3582.401 kb on - strand, within Pf6N2E2_3093 at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.514 kb on - strand at 3582.514 kb on - strand at 3582.514 kb on - strand at 3582.554 kb on + strand at 3582.554 kb on + strand at 3582.555 kb on - strand at 3582.653 kb on + strand at 3582.662 kb on + strand at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.712 kb on - strand, within Pf6N2E2_3094 at 3582.712 kb on - strand, within Pf6N2E2_3094 at 3582.712 kb on - strand, within Pf6N2E2_3094 at 3582.881 kb on + strand, within Pf6N2E2_3094 at 3582.881 kb on + strand, within Pf6N2E2_3094 at 3582.882 kb on - strand, within Pf6N2E2_3094 at 3583.178 kb on + strand, within Pf6N2E2_3094 at 3583.178 kb on + strand, within Pf6N2E2_3094 at 3583.178 kb on + strand, within Pf6N2E2_3094 at 3583.179 kb on - strand, within Pf6N2E2_3094 at 3583.431 kb on - strand, within Pf6N2E2_3095 at 3583.553 kb on + strand at 3583.553 kb on + strand at 3583.554 kb on - strand at 3583.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3 remove 3,580,932 + Pf6N2E2_3092 0.19 -0.7 3,580,932 + Pf6N2E2_3092 0.19 -1.2 3,580,932 + Pf6N2E2_3092 0.19 -0.6 3,580,932 + Pf6N2E2_3092 0.19 -2.1 3,580,932 + Pf6N2E2_3092 0.19 -0.6 3,580,932 + Pf6N2E2_3092 0.19 -0.7 3,580,932 + Pf6N2E2_3092 0.19 +0.3 3,580,932 + Pf6N2E2_3092 0.19 -0.7 3,580,932 + Pf6N2E2_3092 0.19 +0.3 3,580,933 - Pf6N2E2_3092 0.19 -0.5 3,580,933 - Pf6N2E2_3092 0.19 -0.7 3,580,933 - Pf6N2E2_3092 0.19 +0.3 3,580,933 - Pf6N2E2_3092 0.19 +0.3 3,580,933 - Pf6N2E2_3092 0.19 +0.3 3,580,933 - Pf6N2E2_3092 0.19 +0.3 3,580,933 - Pf6N2E2_3092 0.19 -0.7 3,580,933 - Pf6N2E2_3092 0.19 -1.7 3,581,332 - Pf6N2E2_3092 0.55 +0.3 3,581,464 - Pf6N2E2_3092 0.67 +0.3 3,581,682 + Pf6N2E2_3092 0.87 -0.7 3,581,777 + +0.3 3,581,818 - +1.3 3,581,861 - +0.3 3,581,861 - +0.3 3,581,867 - -1.2 3,581,877 - -1.2 3,581,877 - +0.3 3,581,881 + -0.7 3,581,881 + +0.3 3,581,885 - -0.7 3,581,911 - -1.2 3,582,035 - Pf6N2E2_3093 0.18 +1.3 3,582,086 + Pf6N2E2_3093 0.26 +0.3 3,582,086 + Pf6N2E2_3093 0.26 -0.7 3,582,087 - Pf6N2E2_3093 0.26 -0.7 3,582,087 - Pf6N2E2_3093 0.26 +0.3 3,582,087 - Pf6N2E2_3093 0.26 +0.3 3,582,400 + Pf6N2E2_3093 0.75 +1.3 3,582,400 + Pf6N2E2_3093 0.75 +0.3 3,582,401 - Pf6N2E2_3093 0.75 +0.3 3,582,513 + -1.7 3,582,513 + +0.3 3,582,513 + +0.3 3,582,513 + +0.3 3,582,513 + -1.2 3,582,514 - +0.3 3,582,514 - +0.3 3,582,514 - -1.2 3,582,554 + +0.3 3,582,554 + -0.2 3,582,555 - -0.7 3,582,653 + -2.1 3,582,662 + +0.3 3,582,711 + Pf6N2E2_3094 0.11 -2.5 3,582,711 + Pf6N2E2_3094 0.11 +0.3 3,582,711 + Pf6N2E2_3094 0.11 -1.7 3,582,711 + Pf6N2E2_3094 0.11 -1.7 3,582,711 + Pf6N2E2_3094 0.11 +0.3 3,582,711 + Pf6N2E2_3094 0.11 +0.3 3,582,712 - Pf6N2E2_3094 0.11 -0.7 3,582,712 - Pf6N2E2_3094 0.11 -2.4 3,582,712 - Pf6N2E2_3094 0.11 -1.2 3,582,881 + Pf6N2E2_3094 0.32 +0.3 3,582,881 + Pf6N2E2_3094 0.32 +0.3 3,582,882 - Pf6N2E2_3094 0.32 -0.7 3,583,178 + Pf6N2E2_3094 0.70 +0.3 3,583,178 + Pf6N2E2_3094 0.70 +0.3 3,583,178 + Pf6N2E2_3094 0.70 +0.3 3,583,179 - Pf6N2E2_3094 0.70 +0.3 3,583,431 - Pf6N2E2_3095 0.21 -1.0 3,583,553 + -1.2 3,583,553 + +0.3 3,583,554 - -0.7 3,583,554 - +0.3
Or see this region's nucleotide sequence