Experiment: Ying_OrganicAcid7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1837 and Pf6N2E2_1838 are separated by 10 nucleotides Pf6N2E2_1838 and Pf6N2E2_1839 overlap by 1 nucleotides Pf6N2E2_1839 and Pf6N2E2_1840 overlap by 4 nucleotides
Pf6N2E2_1837 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 2,175,429 to 2,176,907
_1837
Pf6N2E2_1838 - 6-hexanolactone hydrolase, at 2,176,918 to 2,177,823
_1838
Pf6N2E2_1839 - 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (EC 1.1.1.-), at 2,177,823 to 2,178,557
_1839
Pf6N2E2_1840 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,178,554 to 2,179,366
_1840
Position (kb)
2176
2177
2178 Strain fitness (log2 ratio)
-1
0
1 at 2176.008 kb on + strand, within Pf6N2E2_1837 at 2176.331 kb on - strand, within Pf6N2E2_1837 at 2176.606 kb on + strand, within Pf6N2E2_1837 at 2176.607 kb on - strand, within Pf6N2E2_1837 at 2176.754 kb on + strand, within Pf6N2E2_1837 at 2176.754 kb on + strand, within Pf6N2E2_1837 at 2176.877 kb on - strand at 2176.924 kb on + strand at 2176.925 kb on - strand at 2177.029 kb on + strand, within Pf6N2E2_1838 at 2177.201 kb on + strand, within Pf6N2E2_1838 at 2177.267 kb on + strand, within Pf6N2E2_1838 at 2177.456 kb on - strand, within Pf6N2E2_1838 at 2177.456 kb on - strand, within Pf6N2E2_1838 at 2177.603 kb on + strand, within Pf6N2E2_1838 at 2177.792 kb on - strand at 2178.115 kb on + strand, within Pf6N2E2_1839 at 2178.225 kb on + strand, within Pf6N2E2_1839 at 2178.254 kb on - strand, within Pf6N2E2_1839 at 2178.254 kb on - strand, within Pf6N2E2_1839 at 2178.302 kb on - strand, within Pf6N2E2_1839 at 2178.435 kb on - strand, within Pf6N2E2_1839 at 2178.500 kb on + strand at 2178.707 kb on - strand, within Pf6N2E2_1840
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3 remove 2,176,008 + Pf6N2E2_1837 0.39 +0.1 2,176,331 - Pf6N2E2_1837 0.61 -0.9 2,176,606 + Pf6N2E2_1837 0.80 +0.1 2,176,607 - Pf6N2E2_1837 0.80 +0.1 2,176,754 + Pf6N2E2_1837 0.90 +0.1 2,176,754 + Pf6N2E2_1837 0.90 +0.1 2,176,877 - +0.1 2,176,924 + +0.1 2,176,925 - -0.9 2,177,029 + Pf6N2E2_1838 0.12 +0.1 2,177,201 + Pf6N2E2_1838 0.31 +0.1 2,177,267 + Pf6N2E2_1838 0.39 +0.1 2,177,456 - Pf6N2E2_1838 0.59 +0.1 2,177,456 - Pf6N2E2_1838 0.59 +0.1 2,177,603 + Pf6N2E2_1838 0.76 +1.1 2,177,792 - +0.1 2,178,115 + Pf6N2E2_1839 0.40 +0.1 2,178,225 + Pf6N2E2_1839 0.55 +0.1 2,178,254 - Pf6N2E2_1839 0.59 -0.9 2,178,254 - Pf6N2E2_1839 0.59 -0.9 2,178,302 - Pf6N2E2_1839 0.65 -0.9 2,178,435 - Pf6N2E2_1839 0.83 +0.1 2,178,500 + +0.1 2,178,707 - Pf6N2E2_1840 0.19 -0.0
Or see this region's nucleotide sequence