Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_148

Experiment: Ying_OrganicAcid7 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_147 and Pf6N2E2_148 are separated by 15 nucleotidesPf6N2E2_148 and Pf6N2E2_149 are separated by 274 nucleotides Pf6N2E2_147 - Outer membrane component of tripartite multidrug resistance system, at 201,559 to 203,013 _147 Pf6N2E2_148 - Membrane fusion component of tripartite multidrug resistance system, at 203,029 to 204,225 _148 Pf6N2E2_149 - Inner membrane component of tripartite multidrug resistance system, at 204,500 to 206,029 _149 Position (kb) 203 204 205Strain fitness (log2 ratio) -2 -1 0 1at 202.100 kb on - strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.130 kb on - strand, within Pf6N2E2_147at 202.217 kb on - strand, within Pf6N2E2_147at 202.498 kb on + strand, within Pf6N2E2_147at 202.681 kb on + strand, within Pf6N2E2_147at 202.681 kb on + strand, within Pf6N2E2_147at 202.681 kb on + strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.820 kb on - strand, within Pf6N2E2_147at 202.840 kb on + strand, within Pf6N2E2_147at 203.007 kb on + strandat 203.008 kb on - strandat 203.008 kb on - strandat 203.021 kb on + strandat 203.022 kb on - strandat 203.022 kb on - strandat 203.022 kb on - strandat 203.022 kb on - strandat 203.161 kb on + strand, within Pf6N2E2_148at 203.161 kb on + strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.194 kb on + strand, within Pf6N2E2_148at 203.194 kb on + strand, within Pf6N2E2_148at 203.194 kb on + strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.383 kb on + strand, within Pf6N2E2_148at 203.384 kb on - strand, within Pf6N2E2_148at 203.676 kb on + strand, within Pf6N2E2_148at 203.677 kb on - strand, within Pf6N2E2_148at 203.702 kb on - strand, within Pf6N2E2_148at 203.863 kb on + strand, within Pf6N2E2_148at 203.864 kb on - strand, within Pf6N2E2_148at 203.864 kb on - strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.882 kb on - strand, within Pf6N2E2_148at 203.936 kb on + strand, within Pf6N2E2_148at 204.137 kb on - strandat 204.137 kb on - strandat 204.137 kb on - strandat 204.703 kb on + strand, within Pf6N2E2_149at 204.790 kb on + strand, within Pf6N2E2_149at 204.790 kb on + strand, within Pf6N2E2_149at 204.791 kb on - strand, within Pf6N2E2_149at 204.828 kb on + strand, within Pf6N2E2_149at 204.829 kb on - strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.993 kb on - strand, within Pf6N2E2_149at 204.993 kb on - strand, within Pf6N2E2_149at 204.993 kb on - strand, within Pf6N2E2_149at 205.107 kb on - strand, within Pf6N2E2_149at 205.107 kb on - strand, within Pf6N2E2_149

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3
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202,100 - Pf6N2E2_147 0.37 -0.8
202,125 + Pf6N2E2_147 0.39 +0.2
202,125 + Pf6N2E2_147 0.39 -1.1
202,125 + Pf6N2E2_147 0.39 +0.2
202,125 + Pf6N2E2_147 0.39 -0.0
202,126 - Pf6N2E2_147 0.39 -0.8
202,126 - Pf6N2E2_147 0.39 -1.8
202,126 - Pf6N2E2_147 0.39 +0.2
202,126 - Pf6N2E2_147 0.39 +0.1
202,126 - Pf6N2E2_147 0.39 +1.2
202,126 - Pf6N2E2_147 0.39 +0.1
202,126 - Pf6N2E2_147 0.39 -0.8
202,129 + Pf6N2E2_147 0.39 +0.2
202,129 + Pf6N2E2_147 0.39 +0.2
202,129 + Pf6N2E2_147 0.39 +0.2
202,129 + Pf6N2E2_147 0.39 +0.2
202,130 - Pf6N2E2_147 0.39 +0.2
202,217 - Pf6N2E2_147 0.45 -0.8
202,498 + Pf6N2E2_147 0.65 +0.2
202,681 + Pf6N2E2_147 0.77 -0.8
202,681 + Pf6N2E2_147 0.77 -0.8
202,681 + Pf6N2E2_147 0.77 -0.8
202,682 - Pf6N2E2_147 0.77 +0.2
202,682 - Pf6N2E2_147 0.77 -0.8
202,682 - Pf6N2E2_147 0.77 +0.2
202,682 - Pf6N2E2_147 0.77 -0.8
202,820 - Pf6N2E2_147 0.87 -0.8
202,840 + Pf6N2E2_147 0.88 +0.2
203,007 + -0.5
203,008 - +0.2
203,008 - +0.2
203,021 + +0.2
203,022 - -0.8
203,022 - +1.1
203,022 - +0.2
203,022 - +0.2
203,161 + Pf6N2E2_148 0.11 +0.2
203,161 + Pf6N2E2_148 0.11 -0.8
203,162 - Pf6N2E2_148 0.11 -0.3
203,162 - Pf6N2E2_148 0.11 -0.8
203,162 - Pf6N2E2_148 0.11 +0.7
203,162 - Pf6N2E2_148 0.11 -0.8
203,162 - Pf6N2E2_148 0.11 -0.0
203,194 + Pf6N2E2_148 0.14 +0.2
203,194 + Pf6N2E2_148 0.14 -0.8
203,194 + Pf6N2E2_148 0.14 +0.4
203,195 - Pf6N2E2_148 0.14 -1.4
203,195 - Pf6N2E2_148 0.14 -1.8
203,195 - Pf6N2E2_148 0.14 -2.1
203,195 - Pf6N2E2_148 0.14 +0.2
203,195 - Pf6N2E2_148 0.14 -0.8
203,195 - Pf6N2E2_148 0.14 +0.2
203,383 + Pf6N2E2_148 0.30 -0.8
203,384 - Pf6N2E2_148 0.30 -0.8
203,676 + Pf6N2E2_148 0.54 +0.2
203,677 - Pf6N2E2_148 0.54 +0.0
203,702 - Pf6N2E2_148 0.56 +0.2
203,863 + Pf6N2E2_148 0.70 +0.0
203,864 - Pf6N2E2_148 0.70 +0.2
203,864 - Pf6N2E2_148 0.70 +0.2
203,881 + Pf6N2E2_148 0.71 +0.2
203,881 + Pf6N2E2_148 0.71 +0.3
203,881 + Pf6N2E2_148 0.71 +1.2
203,882 - Pf6N2E2_148 0.71 +0.2
203,936 + Pf6N2E2_148 0.76 +0.2
204,137 - +0.8
204,137 - -2.0
204,137 - +1.0
204,703 + Pf6N2E2_149 0.13 +0.2
204,790 + Pf6N2E2_149 0.19 -0.8
204,790 + Pf6N2E2_149 0.19 -0.8
204,791 - Pf6N2E2_149 0.19 +0.2
204,828 + Pf6N2E2_149 0.21 -0.8
204,829 - Pf6N2E2_149 0.22 +1.2
204,992 + Pf6N2E2_149 0.32 +0.2
204,992 + Pf6N2E2_149 0.32 +0.2
204,992 + Pf6N2E2_149 0.32 +0.2
204,992 + Pf6N2E2_149 0.32 +0.2
204,992 + Pf6N2E2_149 0.32 -0.8
204,992 + Pf6N2E2_149 0.32 -1.4
204,993 - Pf6N2E2_149 0.32 +0.2
204,993 - Pf6N2E2_149 0.32 -0.8
204,993 - Pf6N2E2_149 0.32 +0.2
205,107 - Pf6N2E2_149 0.40 -0.8
205,107 - Pf6N2E2_149 0.40 +1.4

Or see this region's nucleotide sequence