Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_147

Experiment: Ying_OrganicAcid7 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_145 and Pf6N2E2_146 are separated by 235 nucleotidesPf6N2E2_146 and Pf6N2E2_147 are separated by 4 nucleotidesPf6N2E2_147 and Pf6N2E2_148 are separated by 15 nucleotides Pf6N2E2_145 - putative exported protein, at 197,183 to 200,836 _145 Pf6N2E2_146 - Transcriptional regulator, MarR family, at 201,072 to 201,554 _146 Pf6N2E2_147 - Outer membrane component of tripartite multidrug resistance system, at 201,559 to 203,013 _147 Pf6N2E2_148 - Membrane fusion component of tripartite multidrug resistance system, at 203,029 to 204,225 _148 Position (kb) 201 202 203 204Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 200.608 kb on + strandat 200.754 kb on - strandat 200.774 kb on + strandat 200.774 kb on + strandat 200.775 kb on - strandat 200.775 kb on - strandat 200.819 kb on + strandat 200.819 kb on + strandat 200.819 kb on + strandat 200.820 kb on - strandat 200.820 kb on - strandat 200.820 kb on - strandat 200.820 kb on - strandat 200.820 kb on - strandat 200.820 kb on - strandat 200.834 kb on + strandat 200.835 kb on - strandat 200.835 kb on - strandat 200.881 kb on - strandat 200.959 kb on + strandat 200.959 kb on + strandat 200.960 kb on - strandat 200.960 kb on - strandat 200.960 kb on - strandat 200.977 kb on + strandat 200.993 kb on - strandat 200.996 kb on + strandat 200.997 kb on - strandat 200.997 kb on - strandat 201.018 kb on + strandat 201.039 kb on - strandat 201.039 kb on - strandat 201.039 kb on - strandat 201.039 kb on - strandat 201.052 kb on - strandat 201.167 kb on + strand, within Pf6N2E2_146at 201.167 kb on + strand, within Pf6N2E2_146at 201.552 kb on + strandat 201.552 kb on + strandat 201.553 kb on - strandat 201.553 kb on - strandat 201.616 kb on + strandat 201.838 kb on + strand, within Pf6N2E2_147at 201.839 kb on - strand, within Pf6N2E2_147at 201.940 kb on + strand, within Pf6N2E2_147at 201.940 kb on + strand, within Pf6N2E2_147at 201.940 kb on + strand, within Pf6N2E2_147at 201.941 kb on - strand, within Pf6N2E2_147at 201.941 kb on - strand, within Pf6N2E2_147at 201.941 kb on - strand, within Pf6N2E2_147at 201.960 kb on - strand, within Pf6N2E2_147at 201.960 kb on - strand, within Pf6N2E2_147at 201.976 kb on + strand, within Pf6N2E2_147at 201.976 kb on + strand, within Pf6N2E2_147at 201.976 kb on + strand, within Pf6N2E2_147at 201.976 kb on + strand, within Pf6N2E2_147at 201.977 kb on - strand, within Pf6N2E2_147at 201.977 kb on - strand, within Pf6N2E2_147at 202.100 kb on - strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.130 kb on - strand, within Pf6N2E2_147at 202.217 kb on - strand, within Pf6N2E2_147at 202.498 kb on + strand, within Pf6N2E2_147at 202.681 kb on + strand, within Pf6N2E2_147at 202.681 kb on + strand, within Pf6N2E2_147at 202.681 kb on + strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.820 kb on - strand, within Pf6N2E2_147at 202.840 kb on + strand, within Pf6N2E2_147at 203.007 kb on + strandat 203.008 kb on - strandat 203.008 kb on - strandat 203.021 kb on + strandat 203.022 kb on - strandat 203.022 kb on - strandat 203.022 kb on - strandat 203.022 kb on - strandat 203.161 kb on + strand, within Pf6N2E2_148at 203.161 kb on + strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.194 kb on + strand, within Pf6N2E2_148at 203.194 kb on + strand, within Pf6N2E2_148at 203.194 kb on + strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.383 kb on + strand, within Pf6N2E2_148at 203.384 kb on - strand, within Pf6N2E2_148at 203.676 kb on + strand, within Pf6N2E2_148at 203.677 kb on - strand, within Pf6N2E2_148at 203.702 kb on - strand, within Pf6N2E2_148at 203.863 kb on + strand, within Pf6N2E2_148at 203.864 kb on - strand, within Pf6N2E2_148at 203.864 kb on - strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.882 kb on - strand, within Pf6N2E2_148at 203.936 kb on + strand, within Pf6N2E2_148

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3
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200,608 + -0.9
200,754 - -0.8
200,774 + -0.5
200,774 + -0.6
200,775 - -1.0
200,775 - -0.9
200,819 + -0.9
200,819 + -1.3
200,819 + -0.8
200,820 - -0.4
200,820 - -0.9
200,820 - -0.8
200,820 - -1.1
200,820 - -0.9
200,820 - -0.6
200,834 + -1.8
200,835 - +0.2
200,835 - -2.1
200,881 - -2.6
200,959 + -2.4
200,959 + -1.8
200,960 - -1.4
200,960 - +0.2
200,960 - -0.8
200,977 + +0.2
200,993 - -1.4
200,996 + +0.2
200,997 - +0.2
200,997 - +0.2
201,018 + -0.8
201,039 - -0.8
201,039 - -1.4
201,039 - -0.1
201,039 - -0.2
201,052 - +0.4
201,167 + Pf6N2E2_146 0.20 +0.2
201,167 + Pf6N2E2_146 0.20 -1.7
201,552 + +1.4
201,552 + -1.4
201,553 - +0.2
201,553 - +0.2
201,616 + +0.2
201,838 + Pf6N2E2_147 0.19 +1.2
201,839 - Pf6N2E2_147 0.19 -0.8
201,940 + Pf6N2E2_147 0.26 +1.2
201,940 + Pf6N2E2_147 0.26 +1.2
201,940 + Pf6N2E2_147 0.26 -1.4
201,941 - Pf6N2E2_147 0.26 -2.1
201,941 - Pf6N2E2_147 0.26 +0.2
201,941 - Pf6N2E2_147 0.26 +0.2
201,960 - Pf6N2E2_147 0.28 +0.2
201,960 - Pf6N2E2_147 0.28 -0.8
201,976 + Pf6N2E2_147 0.29 +0.2
201,976 + Pf6N2E2_147 0.29 -7.3
201,976 + Pf6N2E2_147 0.29 -0.8
201,976 + Pf6N2E2_147 0.29 +0.2
201,977 - Pf6N2E2_147 0.29 +0.2
201,977 - Pf6N2E2_147 0.29 +0.2
202,100 - Pf6N2E2_147 0.37 -0.8
202,125 + Pf6N2E2_147 0.39 +0.2
202,125 + Pf6N2E2_147 0.39 -1.1
202,125 + Pf6N2E2_147 0.39 +0.2
202,125 + Pf6N2E2_147 0.39 -0.0
202,126 - Pf6N2E2_147 0.39 -0.8
202,126 - Pf6N2E2_147 0.39 -1.8
202,126 - Pf6N2E2_147 0.39 +0.2
202,126 - Pf6N2E2_147 0.39 +0.1
202,126 - Pf6N2E2_147 0.39 +1.2
202,126 - Pf6N2E2_147 0.39 +0.1
202,126 - Pf6N2E2_147 0.39 -0.8
202,129 + Pf6N2E2_147 0.39 +0.2
202,129 + Pf6N2E2_147 0.39 +0.2
202,129 + Pf6N2E2_147 0.39 +0.2
202,129 + Pf6N2E2_147 0.39 +0.2
202,130 - Pf6N2E2_147 0.39 +0.2
202,217 - Pf6N2E2_147 0.45 -0.8
202,498 + Pf6N2E2_147 0.65 +0.2
202,681 + Pf6N2E2_147 0.77 -0.8
202,681 + Pf6N2E2_147 0.77 -0.8
202,681 + Pf6N2E2_147 0.77 -0.8
202,682 - Pf6N2E2_147 0.77 +0.2
202,682 - Pf6N2E2_147 0.77 -0.8
202,682 - Pf6N2E2_147 0.77 +0.2
202,682 - Pf6N2E2_147 0.77 -0.8
202,820 - Pf6N2E2_147 0.87 -0.8
202,840 + Pf6N2E2_147 0.88 +0.2
203,007 + -0.5
203,008 - +0.2
203,008 - +0.2
203,021 + +0.2
203,022 - -0.8
203,022 - +1.1
203,022 - +0.2
203,022 - +0.2
203,161 + Pf6N2E2_148 0.11 +0.2
203,161 + Pf6N2E2_148 0.11 -0.8
203,162 - Pf6N2E2_148 0.11 -0.3
203,162 - Pf6N2E2_148 0.11 -0.8
203,162 - Pf6N2E2_148 0.11 +0.7
203,162 - Pf6N2E2_148 0.11 -0.8
203,162 - Pf6N2E2_148 0.11 -0.0
203,194 + Pf6N2E2_148 0.14 +0.2
203,194 + Pf6N2E2_148 0.14 -0.8
203,194 + Pf6N2E2_148 0.14 +0.4
203,195 - Pf6N2E2_148 0.14 -1.4
203,195 - Pf6N2E2_148 0.14 -1.8
203,195 - Pf6N2E2_148 0.14 -2.1
203,195 - Pf6N2E2_148 0.14 +0.2
203,195 - Pf6N2E2_148 0.14 -0.8
203,195 - Pf6N2E2_148 0.14 +0.2
203,383 + Pf6N2E2_148 0.30 -0.8
203,384 - Pf6N2E2_148 0.30 -0.8
203,676 + Pf6N2E2_148 0.54 +0.2
203,677 - Pf6N2E2_148 0.54 +0.0
203,702 - Pf6N2E2_148 0.56 +0.2
203,863 + Pf6N2E2_148 0.70 +0.0
203,864 - Pf6N2E2_148 0.70 +0.2
203,864 - Pf6N2E2_148 0.70 +0.2
203,881 + Pf6N2E2_148 0.71 +0.2
203,881 + Pf6N2E2_148 0.71 +0.3
203,881 + Pf6N2E2_148 0.71 +1.2
203,882 - Pf6N2E2_148 0.71 +0.2
203,936 + Pf6N2E2_148 0.76 +0.2

Or see this region's nucleotide sequence