Experiment: Ying_OrganicAcid7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_66 and Pf6N2E2_67 are separated by 54 nucleotides Pf6N2E2_67 and Pf6N2E2_68 are separated by 153 nucleotides
Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136
_66
Pf6N2E2_67 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 107,191 to 108,303
_67
Pf6N2E2_68 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 108,457 to 109,467
_68
Position (kb)
107
108
109 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 106.470 kb on + strand, within Pf6N2E2_66 at 106.470 kb on + strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.543 kb on + strand, within Pf6N2E2_66 at 106.637 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.975 kb on + strand, within Pf6N2E2_66 at 107.076 kb on + strand at 107.111 kb on + strand at 107.177 kb on + strand at 108.356 kb on - strand at 108.441 kb on + strand at 108.450 kb on + strand at 108.451 kb on - strand at 108.451 kb on - strand at 108.451 kb on - strand at 109.084 kb on - strand, within Pf6N2E2_68 at 109.084 kb on - strand, within Pf6N2E2_68 at 109.195 kb on - strand, within Pf6N2E2_68
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3 remove 106,470 + Pf6N2E2_66 0.38 +0.2 106,470 + Pf6N2E2_66 0.38 +0.2 106,475 - Pf6N2E2_66 0.39 -0.8 106,475 - Pf6N2E2_66 0.39 -3.1 106,475 - Pf6N2E2_66 0.39 +0.2 106,529 - Pf6N2E2_66 0.44 +0.2 106,529 - Pf6N2E2_66 0.44 +0.2 106,543 + Pf6N2E2_66 0.45 -4.4 106,637 + Pf6N2E2_66 0.54 +0.2 106,717 + Pf6N2E2_66 0.61 -0.8 106,717 + Pf6N2E2_66 0.61 -1.8 106,717 + Pf6N2E2_66 0.61 +0.2 106,717 + Pf6N2E2_66 0.61 +0.2 106,717 + Pf6N2E2_66 0.61 +0.2 106,717 + Pf6N2E2_66 0.61 +0.2 106,718 - Pf6N2E2_66 0.61 +0.2 106,718 - Pf6N2E2_66 0.61 -7.3 106,718 - Pf6N2E2_66 0.61 +0.2 106,718 - Pf6N2E2_66 0.61 +0.2 106,718 - Pf6N2E2_66 0.61 -6.2 106,718 - Pf6N2E2_66 0.61 +0.2 106,718 - Pf6N2E2_66 0.61 +0.2 106,882 + Pf6N2E2_66 0.76 +0.2 106,882 + Pf6N2E2_66 0.76 -5.0 106,883 - Pf6N2E2_66 0.77 -2.9 106,883 - Pf6N2E2_66 0.77 +0.2 106,883 - Pf6N2E2_66 0.77 -0.8 106,883 - Pf6N2E2_66 0.77 +0.2 106,883 - Pf6N2E2_66 0.77 -2.8 106,975 + Pf6N2E2_66 0.85 -3.2 107,076 + -1.4 107,111 + -0.6 107,177 + +0.2 108,356 - +0.2 108,441 + -1.4 108,450 + +0.2 108,451 - +0.2 108,451 - -0.8 108,451 - +0.2 109,084 - Pf6N2E2_68 0.62 +0.2 109,084 - Pf6N2E2_68 0.62 +0.2 109,195 - Pf6N2E2_68 0.73 -0.4
Or see this region's nucleotide sequence