Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_67

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_66 and Pf6N2E2_67 are separated by 54 nucleotidesPf6N2E2_67 and Pf6N2E2_68 are separated by 153 nucleotides Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136 _66 Pf6N2E2_67 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 107,191 to 108,303 _67 Pf6N2E2_68 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 108,457 to 109,467 _68 Position (kb) 107 108 109Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 106.470 kb on + strand, within Pf6N2E2_66at 106.470 kb on + strand, within Pf6N2E2_66at 106.475 kb on - strand, within Pf6N2E2_66at 106.475 kb on - strand, within Pf6N2E2_66at 106.475 kb on - strand, within Pf6N2E2_66at 106.529 kb on - strand, within Pf6N2E2_66at 106.529 kb on - strand, within Pf6N2E2_66at 106.543 kb on + strand, within Pf6N2E2_66at 106.637 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.882 kb on + strand, within Pf6N2E2_66at 106.882 kb on + strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.975 kb on + strand, within Pf6N2E2_66at 107.076 kb on + strandat 107.111 kb on + strandat 107.177 kb on + strandat 108.356 kb on - strandat 108.441 kb on + strandat 108.450 kb on + strandat 108.451 kb on - strandat 108.451 kb on - strandat 108.451 kb on - strandat 109.084 kb on - strand, within Pf6N2E2_68at 109.084 kb on - strand, within Pf6N2E2_68at 109.195 kb on - strand, within Pf6N2E2_68

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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106,470 + Pf6N2E2_66 0.38 +0.2
106,470 + Pf6N2E2_66 0.38 +0.2
106,475 - Pf6N2E2_66 0.39 -0.8
106,475 - Pf6N2E2_66 0.39 -3.1
106,475 - Pf6N2E2_66 0.39 +0.2
106,529 - Pf6N2E2_66 0.44 +0.2
106,529 - Pf6N2E2_66 0.44 +0.2
106,543 + Pf6N2E2_66 0.45 -4.4
106,637 + Pf6N2E2_66 0.54 +0.2
106,717 + Pf6N2E2_66 0.61 -0.8
106,717 + Pf6N2E2_66 0.61 -1.8
106,717 + Pf6N2E2_66 0.61 +0.2
106,717 + Pf6N2E2_66 0.61 +0.2
106,717 + Pf6N2E2_66 0.61 +0.2
106,717 + Pf6N2E2_66 0.61 +0.2
106,718 - Pf6N2E2_66 0.61 +0.2
106,718 - Pf6N2E2_66 0.61 -7.3
106,718 - Pf6N2E2_66 0.61 +0.2
106,718 - Pf6N2E2_66 0.61 +0.2
106,718 - Pf6N2E2_66 0.61 -6.2
106,718 - Pf6N2E2_66 0.61 +0.2
106,718 - Pf6N2E2_66 0.61 +0.2
106,882 + Pf6N2E2_66 0.76 +0.2
106,882 + Pf6N2E2_66 0.76 -5.0
106,883 - Pf6N2E2_66 0.77 -2.9
106,883 - Pf6N2E2_66 0.77 +0.2
106,883 - Pf6N2E2_66 0.77 -0.8
106,883 - Pf6N2E2_66 0.77 +0.2
106,883 - Pf6N2E2_66 0.77 -2.8
106,975 + Pf6N2E2_66 0.85 -3.2
107,076 + -1.4
107,111 + -0.6
107,177 + +0.2
108,356 - +0.2
108,441 + -1.4
108,450 + +0.2
108,451 - +0.2
108,451 - -0.8
108,451 - +0.2
109,084 - Pf6N2E2_68 0.62 +0.2
109,084 - Pf6N2E2_68 0.62 +0.2
109,195 - Pf6N2E2_68 0.73 -0.4

Or see this region's nucleotide sequence