Experiment: Ying_OrganicAcid7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5675 and Pf6N2E2_5676 are separated by 91 nucleotides Pf6N2E2_5676 and Pf6N2E2_5677 are separated by 186 nucleotides Pf6N2E2_5677 and Pf6N2E2_6158 are separated by 62 nucleotides Pf6N2E2_6158 and Pf6N2E2_6159 are separated by 111 nucleotides Pf6N2E2_6159 and Pf6N2E2_6160 are separated by 75 nucleotides Pf6N2E2_6160 and Pf6N2E2_5678 are separated by 43 nucleotides
Pf6N2E2_5675 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 6,487,329 to 6,489,950
_5675
Pf6N2E2_5676 - Aspartokinase (EC 2.7.2.4), at 6,490,042 to 6,491,283
_5676
Pf6N2E2_5677 - Carbon storage regulator, at 6,491,470 to 6,491,658
_5677
Pf6N2E2_6158 - tRNA-Ser-GCT, at 6,491,721 to 6,491,808
_6158
Pf6N2E2_6159 - tRNA-Arg-ACG, at 6,491,920 to 6,491,993
_6159
Pf6N2E2_6160 - tRNA-Arg-ACG, at 6,492,069 to 6,492,142
_6160
Pf6N2E2_5678 - hypothetical protein, at 6,492,186 to 6,492,692
_5678
Position (kb)
6490
6491
6492 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 6489.934 kb on + strand at 6489.944 kb on + strand at 6489.948 kb on + strand at 6489.948 kb on + strand at 6489.974 kb on + strand at 6490.006 kb on + strand at 6490.006 kb on + strand at 6490.767 kb on + strand, within Pf6N2E2_5676 at 6490.767 kb on + strand, within Pf6N2E2_5676 at 6490.767 kb on + strand, within Pf6N2E2_5676 at 6490.767 kb on + strand, within Pf6N2E2_5676 at 6490.767 kb on + strand, within Pf6N2E2_5676 at 6490.770 kb on + strand, within Pf6N2E2_5676 at 6491.374 kb on + strand at 6491.374 kb on + strand at 6491.377 kb on + strand at 6491.377 kb on + strand at 6491.377 kb on + strand at 6491.377 kb on + strand at 6491.377 kb on + strand at 6491.407 kb on + strand at 6491.407 kb on + strand at 6491.407 kb on + strand at 6491.444 kb on + strand at 6491.444 kb on + strand at 6491.469 kb on + strand at 6491.469 kb on + strand at 6491.469 kb on + strand at 6491.508 kb on + strand, within Pf6N2E2_5677 at 6491.848 kb on + strand at 6491.999 kb on + strand at 6491.999 kb on + strand at 6492.024 kb on + strand at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.260 kb on + strand, within Pf6N2E2_5678 at 6492.260 kb on + strand, within Pf6N2E2_5678 at 6492.260 kb on + strand, within Pf6N2E2_5678 at 6492.261 kb on - strand, within Pf6N2E2_5678 at 6492.262 kb on + strand, within Pf6N2E2_5678
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3 remove 6,489,934 + +0.3 6,489,944 + -1.3 6,489,948 + +0.3 6,489,948 + -1.1 6,489,974 + -2.3 6,490,006 + -0.7 6,490,006 + -1.7 6,490,767 + Pf6N2E2_5676 0.58 -1.3 6,490,767 + Pf6N2E2_5676 0.58 -1.7 6,490,767 + Pf6N2E2_5676 0.58 +0.3 6,490,767 + Pf6N2E2_5676 0.58 -3.9 6,490,767 + Pf6N2E2_5676 0.58 +0.3 6,490,770 + Pf6N2E2_5676 0.59 +0.3 6,491,374 + -0.6 6,491,374 + +0.3 6,491,377 + +0.3 6,491,377 + +0.3 6,491,377 + -0.3 6,491,377 + -0.6 6,491,377 + -0.7 6,491,407 + -0.7 6,491,407 + -0.7 6,491,407 + -2.0 6,491,444 + +0.3 6,491,444 + +0.3 6,491,469 + -1.3 6,491,469 + -0.2 6,491,469 + +0.1 6,491,508 + Pf6N2E2_5677 0.20 -0.0 6,491,848 + +0.3 6,491,999 + -0.3 6,491,999 + -1.1 6,492,024 + -1.3 6,492,256 + Pf6N2E2_5678 0.14 -0.1 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,256 + Pf6N2E2_5678 0.14 -0.7 6,492,256 + Pf6N2E2_5678 0.14 +0.3 6,492,256 + Pf6N2E2_5678 0.14 -0.7 6,492,256 + Pf6N2E2_5678 0.14 -0.7 6,492,256 + Pf6N2E2_5678 0.14 -0.6 6,492,256 + Pf6N2E2_5678 0.14 -0.8 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,257 - Pf6N2E2_5678 0.14 +0.3 6,492,257 - Pf6N2E2_5678 0.14 +0.3 6,492,257 - Pf6N2E2_5678 0.14 +0.3 6,492,257 - Pf6N2E2_5678 0.14 -0.7 6,492,257 - Pf6N2E2_5678 0.14 +0.3 6,492,257 - Pf6N2E2_5678 0.14 +0.3 6,492,257 - Pf6N2E2_5678 0.14 -0.7 6,492,260 + Pf6N2E2_5678 0.15 +0.3 6,492,260 + Pf6N2E2_5678 0.15 -0.7 6,492,260 + Pf6N2E2_5678 0.15 -0.7 6,492,261 - Pf6N2E2_5678 0.15 +0.3 6,492,262 + Pf6N2E2_5678 0.15 +0.3
Or see this region's nucleotide sequence