Experiment: Ying_OrganicAcid7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5461 and Pf6N2E2_5462 are separated by 216 nucleotides Pf6N2E2_5462 and Pf6N2E2_5463 overlap by 4 nucleotides
Pf6N2E2_5461 - Ferredoxin-dependent glutamate synthase (EC 1.4.7.1), at 6,271,734 to 6,273,353
_5461
Pf6N2E2_5462 - Signal transduction histidine kinase, at 6,273,570 to 6,274,976
_5462
Pf6N2E2_5463 - sensor histidine kinase, at 6,274,973 to 6,276,775
_5463
Position (kb)
6273
6274
6275 Strain fitness (log2 ratio)
-2
-1
0
1 at 6272.998 kb on + strand, within Pf6N2E2_5461 at 6272.999 kb on - strand, within Pf6N2E2_5461 at 6272.999 kb on - strand, within Pf6N2E2_5461 at 6273.056 kb on - strand, within Pf6N2E2_5461 at 6273.115 kb on + strand, within Pf6N2E2_5461 at 6273.116 kb on - strand, within Pf6N2E2_5461 at 6273.116 kb on - strand, within Pf6N2E2_5461 at 6273.231 kb on + strand at 6273.231 kb on + strand at 6273.232 kb on - strand at 6273.387 kb on + strand at 6273.388 kb on - strand at 6273.388 kb on - strand at 6273.388 kb on - strand at 6273.388 kb on - strand at 6273.388 kb on - strand at 6273.397 kb on + strand at 6273.398 kb on - strand at 6273.561 kb on + strand at 6273.561 kb on + strand at 6273.562 kb on - strand at 6273.562 kb on - strand at 6273.562 kb on - strand at 6273.562 kb on - strand at 6273.600 kb on + strand at 6273.601 kb on - strand at 6273.941 kb on - strand, within Pf6N2E2_5462 at 6273.941 kb on - strand, within Pf6N2E2_5462 at 6273.964 kb on + strand, within Pf6N2E2_5462 at 6273.965 kb on - strand, within Pf6N2E2_5462 at 6274.258 kb on + strand, within Pf6N2E2_5462 at 6274.259 kb on - strand, within Pf6N2E2_5462 at 6274.618 kb on + strand, within Pf6N2E2_5462 at 6274.618 kb on + strand, within Pf6N2E2_5462 at 6274.618 kb on + strand, within Pf6N2E2_5462 at 6274.739 kb on - strand, within Pf6N2E2_5462 at 6274.739 kb on - strand, within Pf6N2E2_5462 at 6274.935 kb on - strand at 6274.970 kb on + strand at 6274.970 kb on + strand at 6274.970 kb on + strand at 6274.970 kb on + strand at 6274.971 kb on - strand at 6274.971 kb on - strand at 6274.971 kb on - strand at 6274.971 kb on - strand at 6275.120 kb on - strand at 6275.171 kb on - strand, within Pf6N2E2_5463 at 6275.274 kb on + strand, within Pf6N2E2_5463 at 6275.274 kb on + strand, within Pf6N2E2_5463 at 6275.274 kb on + strand, within Pf6N2E2_5463 at 6275.275 kb on - strand, within Pf6N2E2_5463 at 6275.275 kb on - strand, within Pf6N2E2_5463 at 6275.275 kb on - strand, within Pf6N2E2_5463 at 6275.352 kb on + strand, within Pf6N2E2_5463 at 6275.369 kb on - strand, within Pf6N2E2_5463 at 6275.369 kb on - strand, within Pf6N2E2_5463 at 6275.466 kb on + strand, within Pf6N2E2_5463 at 6275.467 kb on - strand, within Pf6N2E2_5463 at 6275.467 kb on - strand, within Pf6N2E2_5463 at 6275.689 kb on + strand, within Pf6N2E2_5463 at 6275.924 kb on + strand, within Pf6N2E2_5463 at 6275.924 kb on + strand, within Pf6N2E2_5463
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3 remove 6,272,998 + Pf6N2E2_5461 0.78 -2.0 6,272,999 - Pf6N2E2_5461 0.78 -1.7 6,272,999 - Pf6N2E2_5461 0.78 +0.3 6,273,056 - Pf6N2E2_5461 0.82 -1.0 6,273,115 + Pf6N2E2_5461 0.85 -1.3 6,273,116 - Pf6N2E2_5461 0.85 -1.3 6,273,116 - Pf6N2E2_5461 0.85 -1.3 6,273,231 + +0.0 6,273,231 + -0.5 6,273,232 - +0.3 6,273,387 + -1.0 6,273,388 - -0.8 6,273,388 - +0.3 6,273,388 - -1.3 6,273,388 - +0.3 6,273,388 - +0.3 6,273,397 + +0.3 6,273,398 - +0.3 6,273,561 + -0.5 6,273,561 + -0.7 6,273,562 - +0.3 6,273,562 - -1.3 6,273,562 - -0.1 6,273,562 - -1.7 6,273,600 + +0.3 6,273,601 - +0.3 6,273,941 - Pf6N2E2_5462 0.26 -2.2 6,273,941 - Pf6N2E2_5462 0.26 -0.7 6,273,964 + Pf6N2E2_5462 0.28 -0.7 6,273,965 - Pf6N2E2_5462 0.28 -0.7 6,274,258 + Pf6N2E2_5462 0.49 -1.3 6,274,259 - Pf6N2E2_5462 0.49 -2.4 6,274,618 + Pf6N2E2_5462 0.74 -0.7 6,274,618 + Pf6N2E2_5462 0.74 -1.7 6,274,618 + Pf6N2E2_5462 0.74 -1.3 6,274,739 - Pf6N2E2_5462 0.83 +0.3 6,274,739 - Pf6N2E2_5462 0.83 -1.7 6,274,935 - -2.5 6,274,970 + -1.3 6,274,970 + +0.3 6,274,970 + -1.7 6,274,970 + +0.3 6,274,971 - +0.3 6,274,971 - -0.7 6,274,971 - +0.3 6,274,971 - +0.3 6,275,120 - +0.3 6,275,171 - Pf6N2E2_5463 0.11 -0.7 6,275,274 + Pf6N2E2_5463 0.17 +0.3 6,275,274 + Pf6N2E2_5463 0.17 -0.7 6,275,274 + Pf6N2E2_5463 0.17 -1.7 6,275,275 - Pf6N2E2_5463 0.17 +0.3 6,275,275 - Pf6N2E2_5463 0.17 +0.3 6,275,275 - Pf6N2E2_5463 0.17 -0.7 6,275,352 + Pf6N2E2_5463 0.21 -0.7 6,275,369 - Pf6N2E2_5463 0.22 -1.1 6,275,369 - Pf6N2E2_5463 0.22 +0.3 6,275,466 + Pf6N2E2_5463 0.27 -1.4 6,275,467 - Pf6N2E2_5463 0.27 -0.3 6,275,467 - Pf6N2E2_5463 0.27 +0.3 6,275,689 + Pf6N2E2_5463 0.40 +0.3 6,275,924 + Pf6N2E2_5463 0.53 -0.3 6,275,924 + Pf6N2E2_5463 0.53 -0.7
Or see this region's nucleotide sequence