Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5462

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5461 and Pf6N2E2_5462 are separated by 216 nucleotidesPf6N2E2_5462 and Pf6N2E2_5463 overlap by 4 nucleotides Pf6N2E2_5461 - Ferredoxin-dependent glutamate synthase (EC 1.4.7.1), at 6,271,734 to 6,273,353 _5461 Pf6N2E2_5462 - Signal transduction histidine kinase, at 6,273,570 to 6,274,976 _5462 Pf6N2E2_5463 - sensor histidine kinase, at 6,274,973 to 6,276,775 _5463 Position (kb) 6273 6274 6275Strain fitness (log2 ratio) -2 -1 0 1at 6272.998 kb on + strand, within Pf6N2E2_5461at 6272.999 kb on - strand, within Pf6N2E2_5461at 6272.999 kb on - strand, within Pf6N2E2_5461at 6273.056 kb on - strand, within Pf6N2E2_5461at 6273.115 kb on + strand, within Pf6N2E2_5461at 6273.116 kb on - strand, within Pf6N2E2_5461at 6273.116 kb on - strand, within Pf6N2E2_5461at 6273.231 kb on + strandat 6273.231 kb on + strandat 6273.232 kb on - strandat 6273.387 kb on + strandat 6273.388 kb on - strandat 6273.388 kb on - strandat 6273.388 kb on - strandat 6273.388 kb on - strandat 6273.388 kb on - strandat 6273.397 kb on + strandat 6273.398 kb on - strandat 6273.561 kb on + strandat 6273.561 kb on + strandat 6273.562 kb on - strandat 6273.562 kb on - strandat 6273.562 kb on - strandat 6273.562 kb on - strandat 6273.600 kb on + strandat 6273.601 kb on - strandat 6273.941 kb on - strand, within Pf6N2E2_5462at 6273.941 kb on - strand, within Pf6N2E2_5462at 6273.964 kb on + strand, within Pf6N2E2_5462at 6273.965 kb on - strand, within Pf6N2E2_5462at 6274.258 kb on + strand, within Pf6N2E2_5462at 6274.259 kb on - strand, within Pf6N2E2_5462at 6274.618 kb on + strand, within Pf6N2E2_5462at 6274.618 kb on + strand, within Pf6N2E2_5462at 6274.618 kb on + strand, within Pf6N2E2_5462at 6274.739 kb on - strand, within Pf6N2E2_5462at 6274.739 kb on - strand, within Pf6N2E2_5462at 6274.935 kb on - strandat 6274.970 kb on + strandat 6274.970 kb on + strandat 6274.970 kb on + strandat 6274.970 kb on + strandat 6274.971 kb on - strandat 6274.971 kb on - strandat 6274.971 kb on - strandat 6274.971 kb on - strandat 6275.120 kb on - strandat 6275.171 kb on - strand, within Pf6N2E2_5463at 6275.274 kb on + strand, within Pf6N2E2_5463at 6275.274 kb on + strand, within Pf6N2E2_5463at 6275.274 kb on + strand, within Pf6N2E2_5463at 6275.275 kb on - strand, within Pf6N2E2_5463at 6275.275 kb on - strand, within Pf6N2E2_5463at 6275.275 kb on - strand, within Pf6N2E2_5463at 6275.352 kb on + strand, within Pf6N2E2_5463at 6275.369 kb on - strand, within Pf6N2E2_5463at 6275.369 kb on - strand, within Pf6N2E2_5463at 6275.466 kb on + strand, within Pf6N2E2_5463at 6275.467 kb on - strand, within Pf6N2E2_5463at 6275.467 kb on - strand, within Pf6N2E2_5463at 6275.689 kb on + strand, within Pf6N2E2_5463at 6275.924 kb on + strand, within Pf6N2E2_5463at 6275.924 kb on + strand, within Pf6N2E2_5463

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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6,272,998 + Pf6N2E2_5461 0.78 -2.0
6,272,999 - Pf6N2E2_5461 0.78 -1.7
6,272,999 - Pf6N2E2_5461 0.78 +0.3
6,273,056 - Pf6N2E2_5461 0.82 -1.0
6,273,115 + Pf6N2E2_5461 0.85 -1.3
6,273,116 - Pf6N2E2_5461 0.85 -1.3
6,273,116 - Pf6N2E2_5461 0.85 -1.3
6,273,231 + +0.0
6,273,231 + -0.5
6,273,232 - +0.3
6,273,387 + -1.0
6,273,388 - -0.8
6,273,388 - +0.3
6,273,388 - -1.3
6,273,388 - +0.3
6,273,388 - +0.3
6,273,397 + +0.3
6,273,398 - +0.3
6,273,561 + -0.5
6,273,561 + -0.7
6,273,562 - +0.3
6,273,562 - -1.3
6,273,562 - -0.1
6,273,562 - -1.7
6,273,600 + +0.3
6,273,601 - +0.3
6,273,941 - Pf6N2E2_5462 0.26 -2.2
6,273,941 - Pf6N2E2_5462 0.26 -0.7
6,273,964 + Pf6N2E2_5462 0.28 -0.7
6,273,965 - Pf6N2E2_5462 0.28 -0.7
6,274,258 + Pf6N2E2_5462 0.49 -1.3
6,274,259 - Pf6N2E2_5462 0.49 -2.4
6,274,618 + Pf6N2E2_5462 0.74 -0.7
6,274,618 + Pf6N2E2_5462 0.74 -1.7
6,274,618 + Pf6N2E2_5462 0.74 -1.3
6,274,739 - Pf6N2E2_5462 0.83 +0.3
6,274,739 - Pf6N2E2_5462 0.83 -1.7
6,274,935 - -2.5
6,274,970 + -1.3
6,274,970 + +0.3
6,274,970 + -1.7
6,274,970 + +0.3
6,274,971 - +0.3
6,274,971 - -0.7
6,274,971 - +0.3
6,274,971 - +0.3
6,275,120 - +0.3
6,275,171 - Pf6N2E2_5463 0.11 -0.7
6,275,274 + Pf6N2E2_5463 0.17 +0.3
6,275,274 + Pf6N2E2_5463 0.17 -0.7
6,275,274 + Pf6N2E2_5463 0.17 -1.7
6,275,275 - Pf6N2E2_5463 0.17 +0.3
6,275,275 - Pf6N2E2_5463 0.17 +0.3
6,275,275 - Pf6N2E2_5463 0.17 -0.7
6,275,352 + Pf6N2E2_5463 0.21 -0.7
6,275,369 - Pf6N2E2_5463 0.22 -1.1
6,275,369 - Pf6N2E2_5463 0.22 +0.3
6,275,466 + Pf6N2E2_5463 0.27 -1.4
6,275,467 - Pf6N2E2_5463 0.27 -0.3
6,275,467 - Pf6N2E2_5463 0.27 +0.3
6,275,689 + Pf6N2E2_5463 0.40 +0.3
6,275,924 + Pf6N2E2_5463 0.53 -0.3
6,275,924 + Pf6N2E2_5463 0.53 -0.7

Or see this region's nucleotide sequence