Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_538

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_536 and Pf6N2E2_537 overlap by 4 nucleotidesPf6N2E2_537 and Pf6N2E2_538 overlap by 1 nucleotidesPf6N2E2_538 and Pf6N2E2_539 are separated by 6 nucleotidesPf6N2E2_539 and Pf6N2E2_540 overlap by 4 nucleotides Pf6N2E2_536 - Na(+) H(+) antiporter subunit D, at 602,844 to 604,529 _536 Pf6N2E2_537 - Na(+) H(+) antiporter subunit C, at 604,526 to 604,870 _537 Pf6N2E2_538 - Na(+) H(+) antiporter subunit A; Na(+) H(+) antiporter subunit B, at 604,870 to 607,794 _538 Pf6N2E2_539 - hypothetical protein, at 607,801 to 608,004 _539 Pf6N2E2_540 - Permease of the drug/metabolite transporter (DMT) superfamily, at 608,001 to 608,870 _540 Position (kb) 604 605 606 607 608Strain fitness (log2 ratio) -2 -1 0 1at 603.970 kb on + strand, within Pf6N2E2_536at 603.970 kb on + strand, within Pf6N2E2_536at 603.971 kb on - strand, within Pf6N2E2_536at 603.971 kb on - strand, within Pf6N2E2_536at 603.971 kb on - strand, within Pf6N2E2_536at 603.971 kb on - strand, within Pf6N2E2_536at 604.027 kb on + strand, within Pf6N2E2_536at 604.027 kb on + strand, within Pf6N2E2_536at 604.028 kb on - strand, within Pf6N2E2_536at 604.028 kb on - strand, within Pf6N2E2_536at 604.028 kb on - strand, within Pf6N2E2_536at 604.028 kb on - strand, within Pf6N2E2_536at 604.098 kb on + strand, within Pf6N2E2_536at 604.099 kb on - strand, within Pf6N2E2_536at 604.099 kb on - strand, within Pf6N2E2_536at 604.150 kb on + strand, within Pf6N2E2_536at 604.330 kb on + strand, within Pf6N2E2_536at 604.372 kb on + strandat 604.372 kb on + strandat 604.373 kb on - strandat 604.668 kb on + strand, within Pf6N2E2_537at 604.668 kb on + strand, within Pf6N2E2_537at 604.668 kb on + strand, within Pf6N2E2_537at 604.668 kb on + strand, within Pf6N2E2_537at 604.669 kb on - strand, within Pf6N2E2_537at 604.669 kb on - strand, within Pf6N2E2_537at 604.761 kb on + strand, within Pf6N2E2_537at 604.769 kb on + strand, within Pf6N2E2_537at 604.769 kb on + strand, within Pf6N2E2_537at 604.770 kb on - strand, within Pf6N2E2_537at 604.838 kb on + strandat 604.839 kb on - strandat 604.923 kb on + strandat 604.991 kb on + strandat 605.201 kb on + strand, within Pf6N2E2_538at 605.201 kb on + strand, within Pf6N2E2_538at 605.201 kb on + strand, within Pf6N2E2_538at 605.201 kb on + strand, within Pf6N2E2_538at 605.201 kb on + strand, within Pf6N2E2_538at 605.201 kb on + strand, within Pf6N2E2_538at 605.201 kb on + strand, within Pf6N2E2_538at 605.202 kb on - strand, within Pf6N2E2_538at 605.202 kb on - strand, within Pf6N2E2_538at 605.202 kb on - strand, within Pf6N2E2_538at 605.282 kb on + strand, within Pf6N2E2_538at 605.282 kb on + strand, within Pf6N2E2_538at 605.283 kb on - strand, within Pf6N2E2_538at 605.335 kb on + strand, within Pf6N2E2_538at 605.597 kb on + strand, within Pf6N2E2_538at 605.597 kb on + strand, within Pf6N2E2_538at 605.598 kb on - strand, within Pf6N2E2_538at 605.598 kb on - strand, within Pf6N2E2_538at 605.643 kb on - strand, within Pf6N2E2_538at 605.912 kb on + strand, within Pf6N2E2_538at 605.912 kb on + strand, within Pf6N2E2_538at 606.237 kb on - strand, within Pf6N2E2_538at 606.314 kb on + strand, within Pf6N2E2_538at 606.314 kb on + strand, within Pf6N2E2_538at 606.521 kb on + strand, within Pf6N2E2_538at 606.954 kb on + strand, within Pf6N2E2_538at 606.954 kb on + strand, within Pf6N2E2_538at 606.955 kb on - strand, within Pf6N2E2_538at 606.980 kb on + strand, within Pf6N2E2_538at 606.980 kb on + strand, within Pf6N2E2_538at 606.980 kb on + strand, within Pf6N2E2_538at 606.981 kb on - strand, within Pf6N2E2_538at 607.067 kb on + strand, within Pf6N2E2_538at 607.067 kb on + strand, within Pf6N2E2_538at 607.195 kb on + strand, within Pf6N2E2_538at 607.195 kb on + strand, within Pf6N2E2_538at 607.195 kb on + strand, within Pf6N2E2_538at 607.195 kb on + strand, within Pf6N2E2_538at 607.195 kb on + strand, within Pf6N2E2_538at 607.195 kb on + strand, within Pf6N2E2_538at 607.196 kb on - strand, within Pf6N2E2_538at 607.233 kb on - strand, within Pf6N2E2_538at 607.233 kb on - strand, within Pf6N2E2_538at 607.233 kb on - strand, within Pf6N2E2_538at 607.233 kb on - strand, within Pf6N2E2_538at 607.233 kb on - strand, within Pf6N2E2_538at 607.311 kb on + strand, within Pf6N2E2_538at 607.312 kb on - strand, within Pf6N2E2_538at 607.312 kb on - strand, within Pf6N2E2_538at 607.312 kb on - strand, within Pf6N2E2_538at 607.312 kb on - strand, within Pf6N2E2_538at 607.312 kb on - strand, within Pf6N2E2_538at 607.313 kb on + strand, within Pf6N2E2_538at 607.313 kb on + strand, within Pf6N2E2_538at 607.313 kb on + strand, within Pf6N2E2_538at 607.313 kb on + strand, within Pf6N2E2_538at 607.313 kb on + strand, within Pf6N2E2_538at 607.313 kb on + strand, within Pf6N2E2_538at 607.313 kb on + strand, within Pf6N2E2_538at 607.313 kb on + strand, within Pf6N2E2_538at 607.313 kb on + strand, within Pf6N2E2_538at 607.314 kb on - strand, within Pf6N2E2_538at 607.314 kb on - strand, within Pf6N2E2_538at 607.314 kb on - strand, within Pf6N2E2_538at 607.314 kb on - strand, within Pf6N2E2_538at 607.314 kb on - strand, within Pf6N2E2_538at 607.314 kb on - strand, within Pf6N2E2_538at 607.314 kb on - strand, within Pf6N2E2_538at 607.499 kb on + strand, within Pf6N2E2_538at 607.499 kb on + strand, within Pf6N2E2_538at 607.499 kb on + strand, within Pf6N2E2_538at 607.499 kb on + strand, within Pf6N2E2_538at 607.499 kb on + strand, within Pf6N2E2_538at 607.499 kb on + strand, within Pf6N2E2_538at 607.499 kb on + strand, within Pf6N2E2_538at 607.499 kb on + strand, within Pf6N2E2_538at 607.499 kb on + strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.500 kb on - strand, within Pf6N2E2_538at 607.511 kb on + strandat 607.512 kb on - strandat 607.616 kb on + strandat 607.616 kb on + strandat 607.617 kb on - strandat 607.617 kb on - strandat 607.617 kb on - strandat 607.617 kb on - strandat 607.617 kb on - strandat 607.617 kb on - strandat 607.777 kb on + strandat 607.777 kb on + strandat 607.777 kb on + strandat 607.783 kb on + strandat 607.784 kb on - strandat 607.784 kb on - strandat 607.784 kb on - strandat 607.794 kb on + strandat 607.794 kb on + strandat 607.795 kb on - strandat 607.795 kb on - strandat 607.795 kb on - strandat 607.802 kb on + strandat 607.803 kb on - strandat 607.829 kb on + strand, within Pf6N2E2_539at 607.830 kb on - strand, within Pf6N2E2_539at 607.830 kb on - strand, within Pf6N2E2_539at 607.876 kb on + strand, within Pf6N2E2_539at 607.877 kb on - strand, within Pf6N2E2_539at 607.883 kb on + strand, within Pf6N2E2_539at 607.883 kb on + strand, within Pf6N2E2_539at 607.883 kb on + strand, within Pf6N2E2_539at 607.883 kb on + strand, within Pf6N2E2_539at 607.883 kb on + strand, within Pf6N2E2_539at 607.884 kb on - strand, within Pf6N2E2_539at 607.884 kb on - strand, within Pf6N2E2_539at 607.884 kb on - strand, within Pf6N2E2_539at 607.884 kb on - strand, within Pf6N2E2_539at 607.884 kb on - strand, within Pf6N2E2_539at 607.884 kb on - strand, within Pf6N2E2_539at 607.884 kb on - strand, within Pf6N2E2_539at 608.173 kb on + strand, within Pf6N2E2_540at 608.173 kb on + strand, within Pf6N2E2_540at 608.173 kb on + strand, within Pf6N2E2_540at 608.173 kb on + strand, within Pf6N2E2_540at 608.173 kb on + strand, within Pf6N2E2_540at 608.173 kb on + strand, within Pf6N2E2_540at 608.173 kb on + strand, within Pf6N2E2_540at 608.174 kb on - strand, within Pf6N2E2_540at 608.174 kb on - strand, within Pf6N2E2_540at 608.174 kb on - strand, within Pf6N2E2_540at 608.174 kb on - strand, within Pf6N2E2_540at 608.174 kb on - strand, within Pf6N2E2_540at 608.174 kb on - strand, within Pf6N2E2_540at 608.174 kb on - strand, within Pf6N2E2_540at 608.174 kb on - strand, within Pf6N2E2_540at 608.174 kb on - strand, within Pf6N2E2_540at 608.174 kb on - strand, within Pf6N2E2_540at 608.596 kb on + strand, within Pf6N2E2_540at 608.596 kb on + strand, within Pf6N2E2_540at 608.597 kb on - strand, within Pf6N2E2_540at 608.597 kb on - strand, within Pf6N2E2_540

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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603,970 + Pf6N2E2_536 0.67 +0.2
603,970 + Pf6N2E2_536 0.67 +0.2
603,971 - Pf6N2E2_536 0.67 +0.2
603,971 - Pf6N2E2_536 0.67 -1.7
603,971 - Pf6N2E2_536 0.67 -0.7
603,971 - Pf6N2E2_536 0.67 +0.2
604,027 + Pf6N2E2_536 0.70 -0.8
604,027 + Pf6N2E2_536 0.70 +0.2
604,028 - Pf6N2E2_536 0.70 +0.2
604,028 - Pf6N2E2_536 0.70 -0.8
604,028 - Pf6N2E2_536 0.70 -0.8
604,028 - Pf6N2E2_536 0.70 -0.8
604,098 + Pf6N2E2_536 0.74 +0.3
604,099 - Pf6N2E2_536 0.74 +0.2
604,099 - Pf6N2E2_536 0.74 -0.9
604,150 + Pf6N2E2_536 0.77 -0.8
604,330 + Pf6N2E2_536 0.88 -1.4
604,372 + +0.2
604,372 + -0.8
604,373 - +0.2
604,668 + Pf6N2E2_537 0.41 +0.2
604,668 + Pf6N2E2_537 0.41 -1.8
604,668 + Pf6N2E2_537 0.41 +0.2
604,668 + Pf6N2E2_537 0.41 +0.3
604,669 - Pf6N2E2_537 0.41 -1.1
604,669 - Pf6N2E2_537 0.41 +0.2
604,761 + Pf6N2E2_537 0.68 +0.2
604,769 + Pf6N2E2_537 0.70 -0.8
604,769 + Pf6N2E2_537 0.70 -2.1
604,770 - Pf6N2E2_537 0.71 +0.2
604,838 + -2.4
604,839 - -1.4
604,923 + +0.2
604,991 + -0.7
605,201 + Pf6N2E2_538 0.11 -1.8
605,201 + Pf6N2E2_538 0.11 -1.3
605,201 + Pf6N2E2_538 0.11 -1.2
605,201 + Pf6N2E2_538 0.11 +0.2
605,201 + Pf6N2E2_538 0.11 -1.4
605,201 + Pf6N2E2_538 0.11 -0.8
605,201 + Pf6N2E2_538 0.11 -0.9
605,202 - Pf6N2E2_538 0.11 -0.5
605,202 - Pf6N2E2_538 0.11 -1.8
605,202 - Pf6N2E2_538 0.11 +0.2
605,282 + Pf6N2E2_538 0.14 -0.8
605,282 + Pf6N2E2_538 0.14 +1.2
605,283 - Pf6N2E2_538 0.14 -2.1
605,335 + Pf6N2E2_538 0.16 +0.2
605,597 + Pf6N2E2_538 0.25 -0.8
605,597 + Pf6N2E2_538 0.25 -0.8
605,598 - Pf6N2E2_538 0.25 +0.2
605,598 - Pf6N2E2_538 0.25 -0.3
605,643 - Pf6N2E2_538 0.26 +0.2
605,912 + Pf6N2E2_538 0.36 +0.2
605,912 + Pf6N2E2_538 0.36 -1.4
606,237 - Pf6N2E2_538 0.47 -0.8
606,314 + Pf6N2E2_538 0.49 +0.2
606,314 + Pf6N2E2_538 0.49 -1.4
606,521 + Pf6N2E2_538 0.56 +0.2
606,954 + Pf6N2E2_538 0.71 +0.2
606,954 + Pf6N2E2_538 0.71 +0.2
606,955 - Pf6N2E2_538 0.71 -0.8
606,980 + Pf6N2E2_538 0.72 +0.2
606,980 + Pf6N2E2_538 0.72 +0.2
606,980 + Pf6N2E2_538 0.72 +0.2
606,981 - Pf6N2E2_538 0.72 -0.8
607,067 + Pf6N2E2_538 0.75 +0.2
607,067 + Pf6N2E2_538 0.75 -0.2
607,195 + Pf6N2E2_538 0.79 +0.2
607,195 + Pf6N2E2_538 0.79 +0.2
607,195 + Pf6N2E2_538 0.79 -0.8
607,195 + Pf6N2E2_538 0.79 +0.2
607,195 + Pf6N2E2_538 0.79 +0.2
607,195 + Pf6N2E2_538 0.79 -1.4
607,196 - Pf6N2E2_538 0.80 +0.2
607,233 - Pf6N2E2_538 0.81 +0.2
607,233 - Pf6N2E2_538 0.81 +0.2
607,233 - Pf6N2E2_538 0.81 +0.2
607,233 - Pf6N2E2_538 0.81 +0.2
607,233 - Pf6N2E2_538 0.81 -1.4
607,311 + Pf6N2E2_538 0.83 +0.2
607,312 - Pf6N2E2_538 0.83 +0.2
607,312 - Pf6N2E2_538 0.83 -0.8
607,312 - Pf6N2E2_538 0.83 +0.2
607,312 - Pf6N2E2_538 0.83 +0.2
607,312 - Pf6N2E2_538 0.83 -0.5
607,313 + Pf6N2E2_538 0.84 +0.2
607,313 + Pf6N2E2_538 0.84 +0.2
607,313 + Pf6N2E2_538 0.84 -2.0
607,313 + Pf6N2E2_538 0.84 +0.2
607,313 + Pf6N2E2_538 0.84 +0.2
607,313 + Pf6N2E2_538 0.84 +0.2
607,313 + Pf6N2E2_538 0.84 -2.4
607,313 + Pf6N2E2_538 0.84 +0.2
607,313 + Pf6N2E2_538 0.84 +0.2
607,314 - Pf6N2E2_538 0.84 +0.2
607,314 - Pf6N2E2_538 0.84 -1.5
607,314 - Pf6N2E2_538 0.84 +0.2
607,314 - Pf6N2E2_538 0.84 -1.1
607,314 - Pf6N2E2_538 0.84 +0.2
607,314 - Pf6N2E2_538 0.84 -1.4
607,314 - Pf6N2E2_538 0.84 -0.6
607,499 + Pf6N2E2_538 0.90 +0.2
607,499 + Pf6N2E2_538 0.90 -0.9
607,499 + Pf6N2E2_538 0.90 +0.2
607,499 + Pf6N2E2_538 0.90 -0.8
607,499 + Pf6N2E2_538 0.90 -0.8
607,499 + Pf6N2E2_538 0.90 +0.2
607,499 + Pf6N2E2_538 0.90 -0.1
607,499 + Pf6N2E2_538 0.90 -1.8
607,499 + Pf6N2E2_538 0.90 -1.6
607,500 - Pf6N2E2_538 0.90 +0.2
607,500 - Pf6N2E2_538 0.90 -1.2
607,500 - Pf6N2E2_538 0.90 +0.2
607,500 - Pf6N2E2_538 0.90 -0.8
607,500 - Pf6N2E2_538 0.90 +0.2
607,500 - Pf6N2E2_538 0.90 -0.9
607,500 - Pf6N2E2_538 0.90 +0.2
607,500 - Pf6N2E2_538 0.90 +0.2
607,500 - Pf6N2E2_538 0.90 +0.2
607,500 - Pf6N2E2_538 0.90 +0.2
607,500 - Pf6N2E2_538 0.90 -0.8
607,511 + +0.2
607,512 - +0.2
607,616 + +0.2
607,616 + +0.2
607,617 - +0.2
607,617 - +0.2
607,617 - +0.2
607,617 - -0.2
607,617 - -0.5
607,617 - +0.2
607,777 + -0.8
607,777 + +0.2
607,777 + -0.8
607,783 + -1.3
607,784 - -1.4
607,784 - -0.4
607,784 - +0.2
607,794 + -0.5
607,794 + +0.2
607,795 - +0.2
607,795 - -0.8
607,795 - -1.1
607,802 + +0.2
607,803 - +0.2
607,829 + Pf6N2E2_539 0.14 -0.8
607,830 - Pf6N2E2_539 0.14 +0.2
607,830 - Pf6N2E2_539 0.14 -0.8
607,876 + Pf6N2E2_539 0.37 +0.2
607,877 - Pf6N2E2_539 0.37 -0.8
607,883 + Pf6N2E2_539 0.40 +0.2
607,883 + Pf6N2E2_539 0.40 +0.2
607,883 + Pf6N2E2_539 0.40 -0.8
607,883 + Pf6N2E2_539 0.40 -0.1
607,883 + Pf6N2E2_539 0.40 -1.1
607,884 - Pf6N2E2_539 0.41 +0.2
607,884 - Pf6N2E2_539 0.41 +0.2
607,884 - Pf6N2E2_539 0.41 -0.2
607,884 - Pf6N2E2_539 0.41 +0.2
607,884 - Pf6N2E2_539 0.41 -0.8
607,884 - Pf6N2E2_539 0.41 +0.2
607,884 - Pf6N2E2_539 0.41 -1.4
608,173 + Pf6N2E2_540 0.20 -0.4
608,173 + Pf6N2E2_540 0.20 -0.8
608,173 + Pf6N2E2_540 0.20 -1.4
608,173 + Pf6N2E2_540 0.20 -0.4
608,173 + Pf6N2E2_540 0.20 -0.2
608,173 + Pf6N2E2_540 0.20 -0.3
608,173 + Pf6N2E2_540 0.20 +0.2
608,174 - Pf6N2E2_540 0.20 -0.8
608,174 - Pf6N2E2_540 0.20 -0.4
608,174 - Pf6N2E2_540 0.20 -0.8
608,174 - Pf6N2E2_540 0.20 -2.1
608,174 - Pf6N2E2_540 0.20 +0.5
608,174 - Pf6N2E2_540 0.20 -0.4
608,174 - Pf6N2E2_540 0.20 -0.1
608,174 - Pf6N2E2_540 0.20 -0.2
608,174 - Pf6N2E2_540 0.20 -0.4
608,174 - Pf6N2E2_540 0.20 +0.2
608,596 + Pf6N2E2_540 0.68 +0.2
608,596 + Pf6N2E2_540 0.68 -1.4
608,597 - Pf6N2E2_540 0.69 +1.2
608,597 - Pf6N2E2_540 0.69 +0.2

Or see this region's nucleotide sequence