Experiment: Ying_OrganicAcid7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4652 and Pf6N2E2_4653 are separated by 19 nucleotides Pf6N2E2_4653 and Pf6N2E2_4654 are separated by 31 nucleotides Pf6N2E2_4654 and Pf6N2E2_4655 are separated by 39 nucleotides
Pf6N2E2_4652 - S-adenosylmethionine synthetase (EC 2.5.1.6), at 5,381,926 to 5,383,116
_4652
Pf6N2E2_4653 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 5,383,136 to 5,384,131
_4653
Pf6N2E2_4654 - hypothetical protein, at 5,384,163 to 5,384,384
_4654
Pf6N2E2_4655 - Transketolase (EC 2.2.1.1), at 5,384,424 to 5,386,421
_4655
Position (kb)
5383
5384
5385 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5383.203 kb on - strand at 5383.209 kb on - strand at 5383.209 kb on - strand at 5383.209 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.431 kb on - strand, within Pf6N2E2_4653 at 5383.431 kb on - strand, within Pf6N2E2_4653 at 5383.493 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.779 kb on - strand, within Pf6N2E2_4653 at 5383.779 kb on - strand, within Pf6N2E2_4653 at 5383.779 kb on - strand, within Pf6N2E2_4653 at 5384.040 kb on - strand at 5384.040 kb on - strand at 5384.040 kb on - strand at 5384.124 kb on - strand at 5384.124 kb on - strand at 5384.132 kb on - strand at 5384.152 kb on - strand at 5384.165 kb on - strand at 5384.165 kb on - strand at 5384.165 kb on - strand at 5384.222 kb on - strand, within Pf6N2E2_4654 at 5384.252 kb on + strand, within Pf6N2E2_4654 at 5384.252 kb on + strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.296 kb on - strand, within Pf6N2E2_4654 at 5384.296 kb on - strand, within Pf6N2E2_4654 at 5384.296 kb on - strand, within Pf6N2E2_4654 at 5384.324 kb on + strand, within Pf6N2E2_4654 at 5384.324 kb on + strand, within Pf6N2E2_4654 at 5384.324 kb on + strand, within Pf6N2E2_4654 at 5384.325 kb on - strand, within Pf6N2E2_4654 at 5384.325 kb on - strand, within Pf6N2E2_4654 at 5384.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3 remove 5,383,203 - -0.7 5,383,209 - +0.1 5,383,209 - -0.6 5,383,209 - -0.6 5,383,213 - -1.2 5,383,213 - +0.4 5,383,213 - -0.3 5,383,213 - -1.2 5,383,213 - -0.6 5,383,213 - +0.4 5,383,332 - Pf6N2E2_4653 0.20 -0.9 5,383,332 - Pf6N2E2_4653 0.20 +0.4 5,383,332 - Pf6N2E2_4653 0.20 -0.6 5,383,332 - Pf6N2E2_4653 0.20 -0.6 5,383,332 - Pf6N2E2_4653 0.20 -1.2 5,383,332 - Pf6N2E2_4653 0.20 -1.6 5,383,431 - Pf6N2E2_4653 0.30 -1.3 5,383,431 - Pf6N2E2_4653 0.30 -1.6 5,383,493 - Pf6N2E2_4653 0.36 -0.6 5,383,650 - Pf6N2E2_4653 0.52 -1.9 5,383,650 - Pf6N2E2_4653 0.52 -0.6 5,383,650 - Pf6N2E2_4653 0.52 -0.3 5,383,650 - Pf6N2E2_4653 0.52 -0.6 5,383,650 - Pf6N2E2_4653 0.52 -0.6 5,383,779 - Pf6N2E2_4653 0.65 -0.6 5,383,779 - Pf6N2E2_4653 0.65 -1.2 5,383,779 - Pf6N2E2_4653 0.65 -0.3 5,384,040 - -0.6 5,384,040 - +0.4 5,384,040 - -1.4 5,384,124 - -1.2 5,384,124 - -0.6 5,384,132 - -0.6 5,384,152 - +0.4 5,384,165 - -0.6 5,384,165 - +0.4 5,384,165 - -1.2 5,384,222 - Pf6N2E2_4654 0.27 -0.6 5,384,252 + Pf6N2E2_4654 0.40 -0.6 5,384,252 + Pf6N2E2_4654 0.40 +0.4 5,384,253 - Pf6N2E2_4654 0.41 -0.6 5,384,253 - Pf6N2E2_4654 0.41 -1.2 5,384,253 - Pf6N2E2_4654 0.41 +0.4 5,384,253 - Pf6N2E2_4654 0.41 -0.6 5,384,253 - Pf6N2E2_4654 0.41 -1.2 5,384,253 - Pf6N2E2_4654 0.41 +0.4 5,384,253 - Pf6N2E2_4654 0.41 +0.4 5,384,253 - Pf6N2E2_4654 0.41 -1.6 5,384,295 + Pf6N2E2_4654 0.59 +0.4 5,384,295 + Pf6N2E2_4654 0.59 -0.6 5,384,295 + Pf6N2E2_4654 0.59 +0.4 5,384,295 + Pf6N2E2_4654 0.59 -0.6 5,384,296 - Pf6N2E2_4654 0.60 -0.6 5,384,296 - Pf6N2E2_4654 0.60 -3.0 5,384,296 - Pf6N2E2_4654 0.60 +0.4 5,384,324 + Pf6N2E2_4654 0.73 -0.1 5,384,324 + Pf6N2E2_4654 0.73 -1.6 5,384,324 + Pf6N2E2_4654 0.73 -0.6 5,384,325 - Pf6N2E2_4654 0.73 -5.1 5,384,325 - Pf6N2E2_4654 0.73 -1.9 5,384,387 + +0.4
Or see this region's nucleotide sequence