Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4419

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4417 and Pf6N2E2_4418 are separated by 13 nucleotidesPf6N2E2_4418 and Pf6N2E2_4419 are separated by 4 nucleotidesPf6N2E2_4419 and Pf6N2E2_4420 are separated by 9 nucleotidesPf6N2E2_4420 and Pf6N2E2_4421 overlap by 18 nucleotidesPf6N2E2_4421 and Pf6N2E2_4422 overlap by 4 nucleotidesPf6N2E2_4422 and Pf6N2E2_4423 are separated by 4 nucleotides Pf6N2E2_4417 - Protein of unknown function DUF484, at 5,150,502 to 5,151,224 _4417 Pf6N2E2_4418 - Diaminopimelate epimerase (EC 5.1.1.7), at 5,151,238 to 5,152,068 _4418 Pf6N2E2_4419 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 5,152,073 to 5,153,320 _4419 Pf6N2E2_4420 - Lipoprotein LppL-related protein, at 5,153,330 to 5,153,452 _4420 Pf6N2E2_4421 - hypothetical protein, at 5,153,435 to 5,153,692 _4421 Pf6N2E2_4422 - Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly, at 5,153,689 to 5,154,021 _4422 Pf6N2E2_4423 - FIG00953342: hypothetical protein, at 5,154,026 to 5,154,244 _4423 Position (kb) 5152 5153 5154Strain fitness (log2 ratio) -2 -1 0 1at 5151.261 kb on - strandat 5151.262 kb on + strandat 5151.263 kb on - strandat 5151.263 kb on - strandat 5151.907 kb on - strand, within Pf6N2E2_4418at 5152.054 kb on - strandat 5153.332 kb on - strandat 5153.335 kb on - strandat 5153.335 kb on - strandat 5153.335 kb on - strandat 5153.335 kb on - strandat 5153.335 kb on - strandat 5153.335 kb on - strandat 5153.335 kb on - strandat 5153.335 kb on - strandat 5153.335 kb on - strandat 5153.335 kb on - strandat 5153.541 kb on - strand, within Pf6N2E2_4421at 5153.541 kb on - strand, within Pf6N2E2_4421at 5153.543 kb on - strand, within Pf6N2E2_4421at 5153.566 kb on - strand, within Pf6N2E2_4421at 5153.611 kb on + strand, within Pf6N2E2_4421at 5153.612 kb on - strand, within Pf6N2E2_4421at 5153.612 kb on - strand, within Pf6N2E2_4421at 5153.613 kb on + strand, within Pf6N2E2_4421at 5153.614 kb on - strand, within Pf6N2E2_4421at 5153.614 kb on - strand, within Pf6N2E2_4421at 5153.614 kb on - strand, within Pf6N2E2_4421at 5153.642 kb on + strand, within Pf6N2E2_4421at 5153.642 kb on + strand, within Pf6N2E2_4421at 5153.643 kb on - strand, within Pf6N2E2_4421at 5153.801 kb on + strand, within Pf6N2E2_4422at 5153.801 kb on + strand, within Pf6N2E2_4422at 5153.905 kb on + strand, within Pf6N2E2_4422at 5153.906 kb on - strand, within Pf6N2E2_4422at 5154.019 kb on + strandat 5154.065 kb on + strand, within Pf6N2E2_4423at 5154.066 kb on - strand, within Pf6N2E2_4423at 5154.066 kb on - strand, within Pf6N2E2_4423at 5154.066 kb on - strand, within Pf6N2E2_4423at 5154.205 kb on + strand, within Pf6N2E2_4423at 5154.242 kb on + strandat 5154.242 kb on + strandat 5154.243 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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5,151,261 - -1.1
5,151,262 + -2.1
5,151,263 - -2.1
5,151,263 - +0.5
5,151,907 - Pf6N2E2_4418 0.81 +0.5
5,152,054 - +0.5
5,153,332 - +0.5
5,153,335 - +0.5
5,153,335 - -0.5
5,153,335 - -0.3
5,153,335 - -0.5
5,153,335 - -1.1
5,153,335 - -0.5
5,153,335 - -0.5
5,153,335 - +0.5
5,153,335 - -1.1
5,153,335 - +0.5
5,153,541 - Pf6N2E2_4421 0.41 +0.5
5,153,541 - Pf6N2E2_4421 0.41 +0.5
5,153,543 - Pf6N2E2_4421 0.42 -0.4
5,153,566 - Pf6N2E2_4421 0.51 -0.5
5,153,611 + Pf6N2E2_4421 0.68 -0.3
5,153,612 - Pf6N2E2_4421 0.69 -1.7
5,153,612 - Pf6N2E2_4421 0.69 -1.5
5,153,613 + Pf6N2E2_4421 0.69 +0.5
5,153,614 - Pf6N2E2_4421 0.69 -0.5
5,153,614 - Pf6N2E2_4421 0.69 -1.1
5,153,614 - Pf6N2E2_4421 0.69 -1.8
5,153,642 + Pf6N2E2_4421 0.80 -0.5
5,153,642 + Pf6N2E2_4421 0.80 +0.5
5,153,643 - Pf6N2E2_4421 0.81 -1.1
5,153,801 + Pf6N2E2_4422 0.34 +0.5
5,153,801 + Pf6N2E2_4422 0.34 +0.5
5,153,905 + Pf6N2E2_4422 0.65 -0.5
5,153,906 - Pf6N2E2_4422 0.65 -0.5
5,154,019 + +0.5
5,154,065 + Pf6N2E2_4423 0.18 -1.9
5,154,066 - Pf6N2E2_4423 0.18 +0.5
5,154,066 - Pf6N2E2_4423 0.18 +0.5
5,154,066 - Pf6N2E2_4423 0.18 +1.5
5,154,205 + Pf6N2E2_4423 0.82 -0.9
5,154,242 + +0.5
5,154,242 + -1.9
5,154,243 - +0.5

Or see this region's nucleotide sequence