Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4048

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4046 and Pf6N2E2_4047 are separated by 84 nucleotidesPf6N2E2_4047 and Pf6N2E2_4048 overlap by 1 nucleotidesPf6N2E2_4048 and Pf6N2E2_4049 are separated by 112 nucleotides Pf6N2E2_4046 - FIG00953482: hypothetical protein, at 4,726,072 to 4,728,033 _4046 Pf6N2E2_4047 - Tryptophan synthase alpha chain (EC 4.2.1.20), at 4,728,118 to 4,728,930 _4047 Pf6N2E2_4048 - Tryptophan synthase beta chain (EC 4.2.1.20), at 4,728,930 to 4,730,162 _4048 Pf6N2E2_4049 - Glycine cleavage system transcriptional activator, at 4,730,275 to 4,731,192 _4049 Position (kb) 4728 4729 4730 4731Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 4728.053 kb on + strandat 4728.054 kb on - strandat 4728.054 kb on - strandat 4728.405 kb on - strand, within Pf6N2E2_4047at 4728.405 kb on - strand, within Pf6N2E2_4047at 4728.405 kb on - strand, within Pf6N2E2_4047at 4728.405 kb on - strand, within Pf6N2E2_4047at 4728.405 kb on - strand, within Pf6N2E2_4047at 4728.405 kb on - strand, within Pf6N2E2_4047at 4728.405 kb on - strand, within Pf6N2E2_4047at 4728.405 kb on - strand, within Pf6N2E2_4047at 4728.408 kb on - strand, within Pf6N2E2_4047at 4728.408 kb on - strand, within Pf6N2E2_4047at 4728.408 kb on - strand, within Pf6N2E2_4047at 4728.409 kb on + strand, within Pf6N2E2_4047at 4728.410 kb on - strand, within Pf6N2E2_4047at 4728.410 kb on - strand, within Pf6N2E2_4047at 4728.410 kb on - strand, within Pf6N2E2_4047at 4728.410 kb on - strand, within Pf6N2E2_4047at 4728.410 kb on - strand, within Pf6N2E2_4047at 4728.410 kb on - strand, within Pf6N2E2_4047at 4728.410 kb on - strand, within Pf6N2E2_4047at 4728.410 kb on - strand, within Pf6N2E2_4047at 4728.410 kb on - strand, within Pf6N2E2_4047at 4728.627 kb on - strand, within Pf6N2E2_4047at 4728.627 kb on - strand, within Pf6N2E2_4047at 4729.157 kb on - strand, within Pf6N2E2_4048at 4729.157 kb on - strand, within Pf6N2E2_4048at 4729.294 kb on - strand, within Pf6N2E2_4048at 4729.294 kb on - strand, within Pf6N2E2_4048at 4729.571 kb on - strand, within Pf6N2E2_4048at 4729.571 kb on - strand, within Pf6N2E2_4048at 4729.571 kb on - strand, within Pf6N2E2_4048at 4729.571 kb on - strand, within Pf6N2E2_4048at 4729.730 kb on - strand, within Pf6N2E2_4048at 4729.730 kb on - strand, within Pf6N2E2_4048at 4729.730 kb on - strand, within Pf6N2E2_4048at 4729.730 kb on - strand, within Pf6N2E2_4048at 4729.730 kb on - strand, within Pf6N2E2_4048at 4729.907 kb on - strand, within Pf6N2E2_4048at 4729.907 kb on - strand, within Pf6N2E2_4048at 4729.907 kb on - strand, within Pf6N2E2_4048at 4729.907 kb on - strand, within Pf6N2E2_4048at 4729.907 kb on - strand, within Pf6N2E2_4048at 4729.907 kb on - strand, within Pf6N2E2_4048at 4729.928 kb on - strand, within Pf6N2E2_4048at 4729.952 kb on - strand, within Pf6N2E2_4048at 4729.952 kb on - strand, within Pf6N2E2_4048at 4729.952 kb on - strand, within Pf6N2E2_4048at 4729.976 kb on - strand, within Pf6N2E2_4048at 4730.084 kb on - strandat 4730.084 kb on - strandat 4730.084 kb on - strandat 4730.084 kb on - strandat 4730.207 kb on + strandat 4730.207 kb on + strandat 4730.208 kb on - strandat 4730.208 kb on - strandat 4730.343 kb on - strandat 4730.384 kb on + strand, within Pf6N2E2_4049at 4730.385 kb on - strand, within Pf6N2E2_4049at 4730.601 kb on + strand, within Pf6N2E2_4049at 4730.602 kb on - strand, within Pf6N2E2_4049at 4730.602 kb on - strand, within Pf6N2E2_4049at 4730.711 kb on + strand, within Pf6N2E2_4049at 4730.711 kb on + strand, within Pf6N2E2_4049at 4730.749 kb on + strand, within Pf6N2E2_4049at 4730.749 kb on + strand, within Pf6N2E2_4049at 4730.749 kb on + strand, within Pf6N2E2_4049at 4730.749 kb on + strand, within Pf6N2E2_4049at 4730.750 kb on - strand, within Pf6N2E2_4049at 4730.750 kb on - strand, within Pf6N2E2_4049at 4730.750 kb on - strand, within Pf6N2E2_4049at 4730.795 kb on - strand, within Pf6N2E2_4049at 4730.953 kb on + strand, within Pf6N2E2_4049at 4730.953 kb on + strand, within Pf6N2E2_4049at 4730.954 kb on - strand, within Pf6N2E2_4049at 4730.954 kb on - strand, within Pf6N2E2_4049at 4730.954 kb on - strand, within Pf6N2E2_4049at 4730.954 kb on - strand, within Pf6N2E2_4049at 4730.954 kb on - strand, within Pf6N2E2_4049at 4730.954 kb on - strand, within Pf6N2E2_4049at 4730.956 kb on + strand, within Pf6N2E2_4049at 4730.956 kb on + strand, within Pf6N2E2_4049at 4730.956 kb on + strand, within Pf6N2E2_4049at 4730.957 kb on - strand, within Pf6N2E2_4049at 4730.957 kb on - strand, within Pf6N2E2_4049at 4730.957 kb on - strand, within Pf6N2E2_4049

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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4,728,053 + +0.4
4,728,054 - +1.0
4,728,054 - -0.6
4,728,405 - Pf6N2E2_4047 0.35 -0.6
4,728,405 - Pf6N2E2_4047 0.35 -3.0
4,728,405 - Pf6N2E2_4047 0.35 +0.4
4,728,405 - Pf6N2E2_4047 0.35 +0.4
4,728,405 - Pf6N2E2_4047 0.35 +0.4
4,728,405 - Pf6N2E2_4047 0.35 +0.4
4,728,405 - Pf6N2E2_4047 0.35 -0.6
4,728,405 - Pf6N2E2_4047 0.35 -0.6
4,728,408 - Pf6N2E2_4047 0.36 +0.4
4,728,408 - Pf6N2E2_4047 0.36 +0.4
4,728,408 - Pf6N2E2_4047 0.36 -1.1
4,728,409 + Pf6N2E2_4047 0.36 +0.4
4,728,410 - Pf6N2E2_4047 0.36 -0.6
4,728,410 - Pf6N2E2_4047 0.36 +0.4
4,728,410 - Pf6N2E2_4047 0.36 -0.6
4,728,410 - Pf6N2E2_4047 0.36 -2.4
4,728,410 - Pf6N2E2_4047 0.36 -1.6
4,728,410 - Pf6N2E2_4047 0.36 -0.6
4,728,410 - Pf6N2E2_4047 0.36 -0.6
4,728,410 - Pf6N2E2_4047 0.36 -0.6
4,728,410 - Pf6N2E2_4047 0.36 -1.1
4,728,627 - Pf6N2E2_4047 0.63 -3.5
4,728,627 - Pf6N2E2_4047 0.63 +0.4
4,729,157 - Pf6N2E2_4048 0.18 +0.4
4,729,157 - Pf6N2E2_4048 0.18 +0.4
4,729,294 - Pf6N2E2_4048 0.30 -1.6
4,729,294 - Pf6N2E2_4048 0.30 -1.1
4,729,571 - Pf6N2E2_4048 0.52 -0.6
4,729,571 - Pf6N2E2_4048 0.52 -0.6
4,729,571 - Pf6N2E2_4048 0.52 -0.6
4,729,571 - Pf6N2E2_4048 0.52 -7.1
4,729,730 - Pf6N2E2_4048 0.65 -0.6
4,729,730 - Pf6N2E2_4048 0.65 -0.6
4,729,730 - Pf6N2E2_4048 0.65 -3.7
4,729,730 - Pf6N2E2_4048 0.65 -2.9
4,729,730 - Pf6N2E2_4048 0.65 +0.4
4,729,907 - Pf6N2E2_4048 0.79 +0.4
4,729,907 - Pf6N2E2_4048 0.79 -2.4
4,729,907 - Pf6N2E2_4048 0.79 +0.4
4,729,907 - Pf6N2E2_4048 0.79 +0.4
4,729,907 - Pf6N2E2_4048 0.79 -6.9
4,729,907 - Pf6N2E2_4048 0.79 -1.1
4,729,928 - Pf6N2E2_4048 0.81 -2.4
4,729,952 - Pf6N2E2_4048 0.83 +0.4
4,729,952 - Pf6N2E2_4048 0.83 +0.4
4,729,952 - Pf6N2E2_4048 0.83 -3.9
4,729,976 - Pf6N2E2_4048 0.85 +0.4
4,730,084 - -0.6
4,730,084 - -1.9
4,730,084 - -0.6
4,730,084 - -3.7
4,730,207 + -0.6
4,730,207 + -1.1
4,730,208 - +0.4
4,730,208 - +0.4
4,730,343 - -0.4
4,730,384 + Pf6N2E2_4049 0.12 -1.9
4,730,385 - Pf6N2E2_4049 0.12 +1.4
4,730,601 + Pf6N2E2_4049 0.36 +0.4
4,730,602 - Pf6N2E2_4049 0.36 -1.1
4,730,602 - Pf6N2E2_4049 0.36 +0.4
4,730,711 + Pf6N2E2_4049 0.47 -0.8
4,730,711 + Pf6N2E2_4049 0.47 -0.3
4,730,749 + Pf6N2E2_4049 0.52 +0.4
4,730,749 + Pf6N2E2_4049 0.52 -0.8
4,730,749 + Pf6N2E2_4049 0.52 -0.9
4,730,749 + Pf6N2E2_4049 0.52 -0.6
4,730,750 - Pf6N2E2_4049 0.52 -0.8
4,730,750 - Pf6N2E2_4049 0.52 -0.6
4,730,750 - Pf6N2E2_4049 0.52 -1.1
4,730,795 - Pf6N2E2_4049 0.57 +0.4
4,730,953 + Pf6N2E2_4049 0.74 -0.4
4,730,953 + Pf6N2E2_4049 0.74 -0.9
4,730,954 - Pf6N2E2_4049 0.74 -0.2
4,730,954 - Pf6N2E2_4049 0.74 +0.4
4,730,954 - Pf6N2E2_4049 0.74 -0.6
4,730,954 - Pf6N2E2_4049 0.74 -1.6
4,730,954 - Pf6N2E2_4049 0.74 +0.4
4,730,954 - Pf6N2E2_4049 0.74 -1.1
4,730,956 + Pf6N2E2_4049 0.74 -0.6
4,730,956 + Pf6N2E2_4049 0.74 -1.1
4,730,956 + Pf6N2E2_4049 0.74 -0.6
4,730,957 - Pf6N2E2_4049 0.74 -0.4
4,730,957 - Pf6N2E2_4049 0.74 +0.4
4,730,957 - Pf6N2E2_4049 0.74 -0.4

Or see this region's nucleotide sequence