Experiment: Ying_OrganicAcid7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3839 and Pf6N2E2_3840 are separated by 42 nucleotides Pf6N2E2_3840 and Pf6N2E2_3841 overlap by 1 nucleotides Pf6N2E2_3841 and Pf6N2E2_3842 overlap by 1 nucleotides Pf6N2E2_3842 and Pf6N2E2_3843 are separated by 281 nucleotides
Pf6N2E2_3839 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 4,472,170 to 4,472,907
_3839
Pf6N2E2_3840 - FIG00956267: hypothetical protein, at 4,472,950 to 4,473,204
_3840
Pf6N2E2_3841 - Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-), at 4,473,204 to 4,473,842
_3841
Pf6N2E2_3842 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 4,473,842 to 4,474,435
_3842
Pf6N2E2_3843 - MFS permease, at 4,474,717 to 4,476,378
_3843
Position (kb)
4473
4474 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 4472.228 kb on + strand at 4472.228 kb on + strand at 4472.229 kb on - strand at 4472.411 kb on + strand, within Pf6N2E2_3839 at 4472.412 kb on - strand, within Pf6N2E2_3839 at 4472.412 kb on - strand, within Pf6N2E2_3839 at 4472.609 kb on + strand, within Pf6N2E2_3839 at 4472.630 kb on + strand, within Pf6N2E2_3839 at 4472.630 kb on + strand, within Pf6N2E2_3839 at 4472.630 kb on + strand, within Pf6N2E2_3839 at 4472.631 kb on - strand, within Pf6N2E2_3839 at 4472.714 kb on - strand, within Pf6N2E2_3839 at 4472.714 kb on - strand, within Pf6N2E2_3839 at 4472.752 kb on - strand, within Pf6N2E2_3839 at 4472.864 kb on - strand at 4472.864 kb on - strand at 4472.890 kb on - strand at 4472.924 kb on + strand at 4472.925 kb on - strand at 4472.925 kb on - strand at 4472.925 kb on - strand at 4472.930 kb on + strand at 4473.003 kb on + strand, within Pf6N2E2_3840 at 4473.004 kb on - strand, within Pf6N2E2_3840 at 4473.004 kb on - strand, within Pf6N2E2_3840 at 4473.038 kb on + strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.041 kb on + strand, within Pf6N2E2_3840 at 4473.041 kb on + strand, within Pf6N2E2_3840 at 4473.041 kb on + strand, within Pf6N2E2_3840 at 4473.332 kb on - strand, within Pf6N2E2_3841 at 4473.332 kb on - strand, within Pf6N2E2_3841 at 4473.376 kb on + strand, within Pf6N2E2_3841 at 4473.377 kb on - strand, within Pf6N2E2_3841 at 4473.377 kb on - strand, within Pf6N2E2_3841 at 4473.377 kb on - strand, within Pf6N2E2_3841 at 4473.377 kb on - strand, within Pf6N2E2_3841 at 4473.379 kb on + strand, within Pf6N2E2_3841 at 4473.379 kb on + strand, within Pf6N2E2_3841 at 4473.380 kb on - strand, within Pf6N2E2_3841 at 4473.380 kb on - strand, within Pf6N2E2_3841 at 4473.380 kb on - strand, within Pf6N2E2_3841 at 4473.380 kb on - strand, within Pf6N2E2_3841 at 4473.394 kb on + strand, within Pf6N2E2_3841 at 4473.395 kb on - strand, within Pf6N2E2_3841 at 4473.606 kb on - strand, within Pf6N2E2_3841 at 4473.606 kb on - strand, within Pf6N2E2_3841 at 4473.844 kb on - strand at 4473.859 kb on + strand at 4473.859 kb on + strand at 4473.860 kb on - strand at 4473.860 kb on - strand at 4473.860 kb on - strand at 4474.068 kb on + strand, within Pf6N2E2_3842 at 4474.068 kb on + strand, within Pf6N2E2_3842 at 4474.146 kb on + strand, within Pf6N2E2_3842 at 4474.146 kb on + strand, within Pf6N2E2_3842 at 4474.147 kb on - strand, within Pf6N2E2_3842 at 4474.147 kb on - strand, within Pf6N2E2_3842 at 4474.147 kb on - strand, within Pf6N2E2_3842 at 4474.216 kb on + strand, within Pf6N2E2_3842 at 4474.423 kb on + strand at 4474.423 kb on + strand at 4474.424 kb on - strand at 4474.424 kb on - strand at 4474.424 kb on - strand at 4474.477 kb on - strand at 4474.478 kb on + strand at 4474.479 kb on - strand at 4474.479 kb on - strand at 4474.479 kb on - strand at 4474.479 kb on - strand at 4474.479 kb on - strand at 4474.479 kb on - strand at 4474.550 kb on + strand at 4474.562 kb on + strand at 4474.562 kb on + strand at 4474.562 kb on + strand at 4474.562 kb on + strand at 4474.562 kb on + strand at 4474.562 kb on + strand at 4474.562 kb on + strand at 4474.563 kb on - strand at 4474.563 kb on - strand at 4474.563 kb on - strand at 4474.563 kb on - strand at 4474.563 kb on - strand at 4474.563 kb on - strand at 4474.563 kb on - strand at 4474.718 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3 remove 4,472,228 + -3.4 4,472,228 + +0.4 4,472,229 - -2.7 4,472,411 + Pf6N2E2_3839 0.33 -0.6 4,472,412 - Pf6N2E2_3839 0.33 -1.1 4,472,412 - Pf6N2E2_3839 0.33 +0.4 4,472,609 + Pf6N2E2_3839 0.59 -4.0 4,472,630 + Pf6N2E2_3839 0.62 -1.1 4,472,630 + Pf6N2E2_3839 0.62 -2.1 4,472,630 + Pf6N2E2_3839 0.62 +0.4 4,472,631 - Pf6N2E2_3839 0.62 -2.7 4,472,714 - Pf6N2E2_3839 0.74 +0.4 4,472,714 - Pf6N2E2_3839 0.74 +0.4 4,472,752 - Pf6N2E2_3839 0.79 -2.1 4,472,864 - -3.0 4,472,864 - -6.6 4,472,890 - -0.6 4,472,924 + +0.4 4,472,925 - -4.3 4,472,925 - -2.6 4,472,925 - -0.6 4,472,930 + +0.4 4,473,003 + Pf6N2E2_3840 0.21 +0.4 4,473,004 - Pf6N2E2_3840 0.21 -1.1 4,473,004 - Pf6N2E2_3840 0.21 +0.4 4,473,038 + Pf6N2E2_3840 0.35 +0.4 4,473,039 - Pf6N2E2_3840 0.35 -0.6 4,473,039 - Pf6N2E2_3840 0.35 +0.3 4,473,039 - Pf6N2E2_3840 0.35 +0.4 4,473,039 - Pf6N2E2_3840 0.35 +0.4 4,473,039 - Pf6N2E2_3840 0.35 +0.4 4,473,039 - Pf6N2E2_3840 0.35 -1.1 4,473,041 + Pf6N2E2_3840 0.36 +0.4 4,473,041 + Pf6N2E2_3840 0.36 +0.4 4,473,041 + Pf6N2E2_3840 0.36 -1.1 4,473,332 - Pf6N2E2_3841 0.20 +0.4 4,473,332 - Pf6N2E2_3841 0.20 -3.8 4,473,376 + Pf6N2E2_3841 0.27 -1.9 4,473,377 - Pf6N2E2_3841 0.27 +0.4 4,473,377 - Pf6N2E2_3841 0.27 -1.1 4,473,377 - Pf6N2E2_3841 0.27 +0.4 4,473,377 - Pf6N2E2_3841 0.27 +0.4 4,473,379 + Pf6N2E2_3841 0.27 -6.8 4,473,379 + Pf6N2E2_3841 0.27 +0.4 4,473,380 - Pf6N2E2_3841 0.28 -0.6 4,473,380 - Pf6N2E2_3841 0.28 +0.4 4,473,380 - Pf6N2E2_3841 0.28 -2.9 4,473,380 - Pf6N2E2_3841 0.28 -4.4 4,473,394 + Pf6N2E2_3841 0.30 -1.6 4,473,395 - Pf6N2E2_3841 0.30 +0.4 4,473,606 - Pf6N2E2_3841 0.63 +0.4 4,473,606 - Pf6N2E2_3841 0.63 +0.4 4,473,844 - +0.4 4,473,859 + +0.4 4,473,859 + -1.9 4,473,860 - -3.3 4,473,860 - -6.5 4,473,860 - +0.4 4,474,068 + Pf6N2E2_3842 0.38 +0.4 4,474,068 + Pf6N2E2_3842 0.38 +0.4 4,474,146 + Pf6N2E2_3842 0.51 +0.4 4,474,146 + Pf6N2E2_3842 0.51 +0.4 4,474,147 - Pf6N2E2_3842 0.51 -6.5 4,474,147 - Pf6N2E2_3842 0.51 -6.4 4,474,147 - Pf6N2E2_3842 0.51 -0.6 4,474,216 + Pf6N2E2_3842 0.63 -1.6 4,474,423 + +0.4 4,474,423 + -1.1 4,474,424 - +0.4 4,474,424 - +0.4 4,474,424 - -1.1 4,474,477 - -1.1 4,474,478 + -0.6 4,474,479 - -0.1 4,474,479 - -1.6 4,474,479 - +0.4 4,474,479 - +0.4 4,474,479 - -0.1 4,474,479 - +0.8 4,474,550 + +1.0 4,474,562 + -0.9 4,474,562 + +0.4 4,474,562 + +0.4 4,474,562 + +0.4 4,474,562 + -0.7 4,474,562 + +0.4 4,474,562 + -1.6 4,474,563 - -0.4 4,474,563 - -0.6 4,474,563 - +0.4 4,474,563 - +0.4 4,474,563 - +0.4 4,474,563 - +0.4 4,474,563 - +0.4 4,474,718 + +0.4
Or see this region's nucleotide sequence