Experiment: Ying_OrganicAcid7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3671 and Pf6N2E2_3672 are separated by 187 nucleotides Pf6N2E2_3672 and Pf6N2E2_3673 are separated by 268 nucleotides Pf6N2E2_3673 and Pf6N2E2_3674 overlap by 6 nucleotides
Pf6N2E2_3671 - ADP-heptose--lipooligosaccharide heptosyltransferase II (EC 2.4.1.-), at 4,273,318 to 4,274,352
_3671
Pf6N2E2_3672 - Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42), at 4,274,540 to 4,277,491
_3672
Pf6N2E2_3673 - hypothetical protein, at 4,277,760 to 4,277,879
_3673
Pf6N2E2_3674 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 4,277,874 to 4,280,519
_3674
Position (kb)
4274
4275
4276
4277
4278 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4273.908 kb on - strand, within Pf6N2E2_3671 at 4274.355 kb on - strand at 4274.355 kb on - strand at 4274.383 kb on - strand at 4274.439 kb on - strand at 4274.439 kb on - strand at 4274.439 kb on - strand at 4274.439 kb on - strand at 4274.439 kb on - strand at 4274.535 kb on - strand at 4274.535 kb on - strand at 4274.668 kb on - strand at 4274.668 kb on - strand at 4274.668 kb on - strand at 4274.668 kb on - strand at 4275.168 kb on + strand, within Pf6N2E2_3672 at 4275.168 kb on + strand, within Pf6N2E2_3672 at 4275.168 kb on + strand, within Pf6N2E2_3672 at 4275.168 kb on + strand, within Pf6N2E2_3672 at 4275.169 kb on - strand, within Pf6N2E2_3672 at 4275.169 kb on - strand, within Pf6N2E2_3672 at 4275.169 kb on - strand, within Pf6N2E2_3672 at 4275.361 kb on + strand, within Pf6N2E2_3672 at 4275.362 kb on - strand at 4275.483 kb on + strand, within Pf6N2E2_3672 at 4275.483 kb on + strand, within Pf6N2E2_3672 at 4275.483 kb on + strand, within Pf6N2E2_3672 at 4275.796 kb on - strand, within Pf6N2E2_3672 at 4275.796 kb on - strand, within Pf6N2E2_3672 at 4276.159 kb on - strand, within Pf6N2E2_3672 at 4276.159 kb on - strand, within Pf6N2E2_3672 at 4276.159 kb on - strand, within Pf6N2E2_3672 at 4276.289 kb on - strand, within Pf6N2E2_3672 at 4276.289 kb on - strand, within Pf6N2E2_3672 at 4276.290 kb on + strand, within Pf6N2E2_3672 at 4276.290 kb on + strand, within Pf6N2E2_3672 at 4276.291 kb on - strand, within Pf6N2E2_3672 at 4276.306 kb on - strand, within Pf6N2E2_3672 at 4276.344 kb on + strand, within Pf6N2E2_3672 at 4276.344 kb on + strand, within Pf6N2E2_3672 at 4276.576 kb on - strand, within Pf6N2E2_3672 at 4276.686 kb on + strand, within Pf6N2E2_3672 at 4276.687 kb on - strand, within Pf6N2E2_3672 at 4276.687 kb on - strand, within Pf6N2E2_3672 at 4276.689 kb on + strand, within Pf6N2E2_3672 at 4276.689 kb on + strand, within Pf6N2E2_3672 at 4276.690 kb on - strand, within Pf6N2E2_3672 at 4276.690 kb on - strand, within Pf6N2E2_3672 at 4276.690 kb on - strand, within Pf6N2E2_3672 at 4276.690 kb on - strand, within Pf6N2E2_3672 at 4277.022 kb on + strand, within Pf6N2E2_3672 at 4277.022 kb on + strand, within Pf6N2E2_3672 at 4277.023 kb on - strand, within Pf6N2E2_3672 at 4277.023 kb on - strand, within Pf6N2E2_3672 at 4277.023 kb on - strand, within Pf6N2E2_3672 at 4277.023 kb on - strand, within Pf6N2E2_3672 at 4277.034 kb on + strand, within Pf6N2E2_3672 at 4277.240 kb on - strand at 4277.279 kb on + strand at 4277.330 kb on - strand at 4277.527 kb on + strand at 4277.543 kb on + strand at 4277.544 kb on - strand at 4277.549 kb on - strand at 4277.549 kb on - strand at 4277.717 kb on + strand at 4277.719 kb on + strand at 4277.719 kb on + strand at 4277.761 kb on + strand at 4278.225 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3 remove 4,273,908 - Pf6N2E2_3671 0.57 +0.4 4,274,355 - -1.3 4,274,355 - -1.2 4,274,383 - -0.2 4,274,439 - +0.4 4,274,439 - +0.4 4,274,439 - +0.4 4,274,439 - +0.4 4,274,439 - -0.0 4,274,535 - +0.4 4,274,535 - +2.0 4,274,668 - -0.6 4,274,668 - -0.6 4,274,668 - +0.4 4,274,668 - -0.6 4,275,168 + Pf6N2E2_3672 0.21 +0.4 4,275,168 + Pf6N2E2_3672 0.21 -2.9 4,275,168 + Pf6N2E2_3672 0.21 +0.4 4,275,168 + Pf6N2E2_3672 0.21 +0.4 4,275,169 - Pf6N2E2_3672 0.21 -1.9 4,275,169 - Pf6N2E2_3672 0.21 -3.0 4,275,169 - Pf6N2E2_3672 0.21 -1.0 4,275,361 + Pf6N2E2_3672 0.28 +0.4 4,275,362 - +0.4 4,275,483 + Pf6N2E2_3672 0.32 -1.2 4,275,483 + Pf6N2E2_3672 0.32 +0.4 4,275,483 + Pf6N2E2_3672 0.32 -2.0 4,275,796 - Pf6N2E2_3672 0.43 -4.5 4,275,796 - Pf6N2E2_3672 0.43 -1.6 4,276,159 - Pf6N2E2_3672 0.55 -3.8 4,276,159 - Pf6N2E2_3672 0.55 -1.9 4,276,159 - Pf6N2E2_3672 0.55 +0.4 4,276,289 - Pf6N2E2_3672 0.59 -3.2 4,276,289 - Pf6N2E2_3672 0.59 +0.4 4,276,290 + Pf6N2E2_3672 0.59 -1.6 4,276,290 + Pf6N2E2_3672 0.59 +0.4 4,276,291 - Pf6N2E2_3672 0.59 -2.5 4,276,306 - Pf6N2E2_3672 0.60 -3.0 4,276,344 + Pf6N2E2_3672 0.61 -0.6 4,276,344 + Pf6N2E2_3672 0.61 -0.6 4,276,576 - Pf6N2E2_3672 0.69 +0.4 4,276,686 + Pf6N2E2_3672 0.73 -0.6 4,276,687 - Pf6N2E2_3672 0.73 +0.4 4,276,687 - Pf6N2E2_3672 0.73 -2.8 4,276,689 + Pf6N2E2_3672 0.73 -0.9 4,276,689 + Pf6N2E2_3672 0.73 -0.6 4,276,690 - Pf6N2E2_3672 0.73 -2.7 4,276,690 - Pf6N2E2_3672 0.73 -2.9 4,276,690 - Pf6N2E2_3672 0.73 -0.6 4,276,690 - Pf6N2E2_3672 0.73 -1.2 4,277,022 + Pf6N2E2_3672 0.84 -2.4 4,277,022 + Pf6N2E2_3672 0.84 -3.2 4,277,023 - Pf6N2E2_3672 0.84 +0.4 4,277,023 - Pf6N2E2_3672 0.84 -0.6 4,277,023 - Pf6N2E2_3672 0.84 -1.2 4,277,023 - Pf6N2E2_3672 0.84 +0.4 4,277,034 + Pf6N2E2_3672 0.84 +0.4 4,277,240 - -3.3 4,277,279 + -1.2 4,277,330 - -3.3 4,277,527 + -0.6 4,277,543 + -0.4 4,277,544 - -4.8 4,277,549 - -4.4 4,277,549 - -4.5 4,277,717 + -0.6 4,277,719 + +0.4 4,277,719 + -0.6 4,277,761 + -0.6 4,278,225 + -4.0
Or see this region's nucleotide sequence