Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2778

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2776 and Pf6N2E2_2777 overlap by 4 nucleotidesPf6N2E2_2777 and Pf6N2E2_2778 are separated by 12 nucleotidesPf6N2E2_2778 and Pf6N2E2_2779 are separated by 11 nucleotidesPf6N2E2_2779 and Pf6N2E2_2780 overlap by 49 nucleotidesPf6N2E2_2780 and Pf6N2E2_2781 are separated by 158 nucleotides Pf6N2E2_2776 - Urea carboxylase-related ABC transporter, permease protein, at 3,264,523 to 3,265,338 _2776 Pf6N2E2_2777 - Urea carboxylase-related ABC transporter, ATPase protein, at 3,265,335 to 3,266,123 _2777 Pf6N2E2_2778 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,266,136 to 3,266,861 _2778 Pf6N2E2_2779 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,266,873 to 3,267,514 _2779 Pf6N2E2_2780 - hypothetical protein, at 3,267,466 to 3,267,582 _2780 Pf6N2E2_2781 - Urea carboxylase (EC 6.3.4.6), at 3,267,741 to 3,271,421 _2781 Position (kb) 3266 3267Strain fitness (log2 ratio) -2 -1 0 1at 3265.214 kb on - strand, within Pf6N2E2_2776at 3265.241 kb on - strand, within Pf6N2E2_2776at 3265.318 kb on + strandat 3265.319 kb on - strandat 3265.319 kb on - strandat 3265.371 kb on + strandat 3265.371 kb on + strandat 3265.371 kb on + strandat 3265.579 kb on - strand, within Pf6N2E2_2777at 3265.705 kb on + strand, within Pf6N2E2_2777at 3265.705 kb on + strand, within Pf6N2E2_2777at 3265.706 kb on - strand, within Pf6N2E2_2777at 3265.706 kb on - strand, within Pf6N2E2_2777at 3265.706 kb on - strand, within Pf6N2E2_2777at 3265.739 kb on + strand, within Pf6N2E2_2777at 3265.740 kb on - strand, within Pf6N2E2_2777at 3265.743 kb on + strand, within Pf6N2E2_2777at 3265.744 kb on - strand, within Pf6N2E2_2777at 3265.744 kb on - strand, within Pf6N2E2_2777at 3266.014 kb on - strand, within Pf6N2E2_2777at 3266.031 kb on + strand, within Pf6N2E2_2777at 3266.031 kb on + strand, within Pf6N2E2_2777at 3266.031 kb on + strand, within Pf6N2E2_2777at 3266.032 kb on - strand, within Pf6N2E2_2777at 3266.032 kb on - strand, within Pf6N2E2_2777at 3266.032 kb on - strand, within Pf6N2E2_2777at 3266.032 kb on - strand, within Pf6N2E2_2777at 3266.032 kb on - strand, within Pf6N2E2_2777at 3266.114 kb on + strandat 3266.159 kb on + strandat 3266.275 kb on + strand, within Pf6N2E2_2778at 3266.661 kb on + strand, within Pf6N2E2_2778at 3266.661 kb on + strand, within Pf6N2E2_2778at 3266.673 kb on + strand, within Pf6N2E2_2778at 3266.674 kb on - strand, within Pf6N2E2_2778at 3267.029 kb on + strand, within Pf6N2E2_2779at 3267.095 kb on + strand, within Pf6N2E2_2779at 3267.096 kb on - strand, within Pf6N2E2_2779at 3267.096 kb on - strand, within Pf6N2E2_2779at 3267.096 kb on - strand, within Pf6N2E2_2779at 3267.119 kb on + strand, within Pf6N2E2_2779at 3267.119 kb on + strand, within Pf6N2E2_2779at 3267.119 kb on + strand, within Pf6N2E2_2779at 3267.120 kb on - strand, within Pf6N2E2_2779at 3267.120 kb on - strand, within Pf6N2E2_2779at 3267.221 kb on + strand, within Pf6N2E2_2779at 3267.239 kb on + strand, within Pf6N2E2_2779at 3267.239 kb on + strand, within Pf6N2E2_2779at 3267.240 kb on - strand, within Pf6N2E2_2779at 3267.263 kb on + strand, within Pf6N2E2_2779at 3267.263 kb on + strand, within Pf6N2E2_2779at 3267.264 kb on - strand, within Pf6N2E2_2779at 3267.264 kb on - strand, within Pf6N2E2_2779at 3267.264 kb on - strand, within Pf6N2E2_2779at 3267.264 kb on - strand, within Pf6N2E2_2779at 3267.398 kb on + strand, within Pf6N2E2_2779at 3267.399 kb on - strand, within Pf6N2E2_2779at 3267.399 kb on - strand, within Pf6N2E2_2779at 3267.399 kb on - strand, within Pf6N2E2_2779at 3267.399 kb on - strand, within Pf6N2E2_2779at 3267.476 kb on + strandat 3267.476 kb on + strandat 3267.580 kb on + strandat 3267.580 kb on + strandat 3267.834 kb on + strandat 3267.834 kb on + strandat 3267.834 kb on + strandat 3267.835 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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3,265,214 - Pf6N2E2_2776 0.85 +0.2
3,265,241 - Pf6N2E2_2776 0.88 +0.3
3,265,318 + +0.3
3,265,319 - +0.3
3,265,319 - +0.3
3,265,371 + -1.5
3,265,371 + -1.1
3,265,371 + -1.3
3,265,579 - Pf6N2E2_2777 0.31 -1.0
3,265,705 + Pf6N2E2_2777 0.47 -0.7
3,265,705 + Pf6N2E2_2777 0.47 +0.3
3,265,706 - Pf6N2E2_2777 0.47 +0.3
3,265,706 - Pf6N2E2_2777 0.47 +0.3
3,265,706 - Pf6N2E2_2777 0.47 -0.7
3,265,739 + Pf6N2E2_2777 0.51 -0.7
3,265,740 - Pf6N2E2_2777 0.51 -0.4
3,265,743 + Pf6N2E2_2777 0.52 +0.3
3,265,744 - Pf6N2E2_2777 0.52 -0.7
3,265,744 - Pf6N2E2_2777 0.52 -0.8
3,266,014 - Pf6N2E2_2777 0.86 -0.7
3,266,031 + Pf6N2E2_2777 0.88 +0.3
3,266,031 + Pf6N2E2_2777 0.88 +0.2
3,266,031 + Pf6N2E2_2777 0.88 +0.3
3,266,032 - Pf6N2E2_2777 0.88 +0.3
3,266,032 - Pf6N2E2_2777 0.88 +0.3
3,266,032 - Pf6N2E2_2777 0.88 +0.3
3,266,032 - Pf6N2E2_2777 0.88 -0.7
3,266,032 - Pf6N2E2_2777 0.88 -0.7
3,266,114 + -2.5
3,266,159 + +0.3
3,266,275 + Pf6N2E2_2778 0.19 +0.3
3,266,661 + Pf6N2E2_2778 0.72 -0.7
3,266,661 + Pf6N2E2_2778 0.72 -0.7
3,266,673 + Pf6N2E2_2778 0.74 -0.7
3,266,674 - Pf6N2E2_2778 0.74 -1.7
3,267,029 + Pf6N2E2_2779 0.24 -0.3
3,267,095 + Pf6N2E2_2779 0.35 -0.7
3,267,096 - Pf6N2E2_2779 0.35 +0.3
3,267,096 - Pf6N2E2_2779 0.35 -0.7
3,267,096 - Pf6N2E2_2779 0.35 +0.3
3,267,119 + Pf6N2E2_2779 0.38 +0.3
3,267,119 + Pf6N2E2_2779 0.38 +0.3
3,267,119 + Pf6N2E2_2779 0.38 +0.0
3,267,120 - Pf6N2E2_2779 0.38 -0.7
3,267,120 - Pf6N2E2_2779 0.38 -1.3
3,267,221 + Pf6N2E2_2779 0.54 -0.7
3,267,239 + Pf6N2E2_2779 0.57 +0.3
3,267,239 + Pf6N2E2_2779 0.57 +0.3
3,267,240 - Pf6N2E2_2779 0.57 +0.3
3,267,263 + Pf6N2E2_2779 0.61 -1.3
3,267,263 + Pf6N2E2_2779 0.61 +0.3
3,267,264 - Pf6N2E2_2779 0.61 -0.2
3,267,264 - Pf6N2E2_2779 0.61 -0.7
3,267,264 - Pf6N2E2_2779 0.61 -2.0
3,267,264 - Pf6N2E2_2779 0.61 +0.3
3,267,398 + Pf6N2E2_2779 0.82 -0.7
3,267,399 - Pf6N2E2_2779 0.82 -0.7
3,267,399 - Pf6N2E2_2779 0.82 -0.7
3,267,399 - Pf6N2E2_2779 0.82 -0.9
3,267,399 - Pf6N2E2_2779 0.82 -0.7
3,267,476 + -1.3
3,267,476 + -0.7
3,267,580 + -0.7
3,267,580 + -2.6
3,267,834 + +0.3
3,267,834 + +0.3
3,267,834 + -0.9
3,267,835 - -0.3

Or see this region's nucleotide sequence