Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_189

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_188 and Pf6N2E2_189 are separated by 150 nucleotidesPf6N2E2_189 and Pf6N2E2_190 are separated by 14 nucleotides Pf6N2E2_188 - Formamidase (EC 3.5.1.49), at 240,740 to 241,969 _188 Pf6N2E2_189 - regulatory protein, putative, at 242,120 to 242,353 _189 Pf6N2E2_190 - Aliphatic amidase AmiE (EC 3.5.1.4), at 242,368 to 243,411 _190 Position (kb) 242 243Strain fitness (log2 ratio) -2 -1 0 1 2at 241.405 kb on + strand, within Pf6N2E2_188at 241.406 kb on - strand, within Pf6N2E2_188at 241.457 kb on + strand, within Pf6N2E2_188at 241.558 kb on + strand, within Pf6N2E2_188at 241.644 kb on - strand, within Pf6N2E2_188at 241.652 kb on + strand, within Pf6N2E2_188at 241.653 kb on - strand, within Pf6N2E2_188at 241.653 kb on - strand, within Pf6N2E2_188at 241.653 kb on - strand, within Pf6N2E2_188at 241.669 kb on + strand, within Pf6N2E2_188at 241.752 kb on - strand, within Pf6N2E2_188at 241.752 kb on - strand, within Pf6N2E2_188at 241.788 kb on - strand, within Pf6N2E2_188at 241.822 kb on + strand, within Pf6N2E2_188at 242.015 kb on + strandat 242.015 kb on + strandat 242.015 kb on + strandat 242.016 kb on - strandat 242.021 kb on + strandat 242.022 kb on - strandat 242.222 kb on + strand, within Pf6N2E2_189at 242.222 kb on + strand, within Pf6N2E2_189at 242.318 kb on - strand, within Pf6N2E2_189at 242.318 kb on - strand, within Pf6N2E2_189at 242.351 kb on + strandat 242.360 kb on + strandat 242.360 kb on + strandat 242.360 kb on + strandat 242.360 kb on + strandat 242.383 kb on - strandat 242.425 kb on + strandat 242.445 kb on + strandat 242.445 kb on + strandat 242.445 kb on + strandat 242.445 kb on + strandat 242.446 kb on - strandat 242.545 kb on + strand, within Pf6N2E2_190at 242.566 kb on + strand, within Pf6N2E2_190at 242.566 kb on + strand, within Pf6N2E2_190at 242.567 kb on - strand, within Pf6N2E2_190at 242.567 kb on - strand, within Pf6N2E2_190at 242.601 kb on + strand, within Pf6N2E2_190at 242.713 kb on + strand, within Pf6N2E2_190at 242.714 kb on - strand, within Pf6N2E2_190at 242.789 kb on + strand, within Pf6N2E2_190at 242.790 kb on - strand, within Pf6N2E2_190at 242.790 kb on - strand, within Pf6N2E2_190at 242.790 kb on - strand, within Pf6N2E2_190at 242.818 kb on + strand, within Pf6N2E2_190at 242.819 kb on - strand, within Pf6N2E2_190at 242.819 kb on - strand, within Pf6N2E2_190at 242.879 kb on - strand, within Pf6N2E2_190at 242.941 kb on + strand, within Pf6N2E2_190at 242.941 kb on + strand, within Pf6N2E2_190at 242.941 kb on + strand, within Pf6N2E2_190at 242.941 kb on + strand, within Pf6N2E2_190at 242.941 kb on + strand, within Pf6N2E2_190at 242.942 kb on - strand, within Pf6N2E2_190at 242.942 kb on - strand, within Pf6N2E2_190at 242.942 kb on - strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 243.007 kb on + strand, within Pf6N2E2_190at 243.046 kb on + strand, within Pf6N2E2_190at 243.046 kb on + strand, within Pf6N2E2_190at 243.130 kb on + strand, within Pf6N2E2_190at 243.130 kb on + strand, within Pf6N2E2_190at 243.130 kb on + strand, within Pf6N2E2_190at 243.130 kb on + strand, within Pf6N2E2_190at 243.229 kb on + strand, within Pf6N2E2_190at 243.229 kb on + strand, within Pf6N2E2_190at 243.229 kb on + strand, within Pf6N2E2_190at 243.229 kb on + strand, within Pf6N2E2_190at 243.230 kb on - strand, within Pf6N2E2_190at 243.340 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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241,405 + Pf6N2E2_188 0.54 +0.2
241,406 - Pf6N2E2_188 0.54 -0.6
241,457 + Pf6N2E2_188 0.58 +0.2
241,558 + Pf6N2E2_188 0.67 -0.8
241,644 - Pf6N2E2_188 0.73 -1.4
241,652 + Pf6N2E2_188 0.74 -0.8
241,653 - Pf6N2E2_188 0.74 -0.8
241,653 - Pf6N2E2_188 0.74 +0.2
241,653 - Pf6N2E2_188 0.74 +0.2
241,669 + Pf6N2E2_188 0.76 -2.7
241,752 - Pf6N2E2_188 0.82 +0.2
241,752 - Pf6N2E2_188 0.82 -1.4
241,788 - Pf6N2E2_188 0.85 +0.2
241,822 + Pf6N2E2_188 0.88 +0.2
242,015 + -0.8
242,015 + +0.2
242,015 + -1.1
242,016 - -0.8
242,021 + -0.8
242,022 - +0.2
242,222 + Pf6N2E2_189 0.44 +0.2
242,222 + Pf6N2E2_189 0.44 +0.2
242,318 - Pf6N2E2_189 0.85 -1.8
242,318 - Pf6N2E2_189 0.85 -0.8
242,351 + -0.8
242,360 + -0.8
242,360 + -0.8
242,360 + -0.8
242,360 + +0.2
242,383 - -1.4
242,425 + +0.2
242,445 + -0.4
242,445 + -0.8
242,445 + +0.2
242,445 + +0.2
242,446 - +0.2
242,545 + Pf6N2E2_190 0.17 -0.8
242,566 + Pf6N2E2_190 0.19 +0.2
242,566 + Pf6N2E2_190 0.19 +0.2
242,567 - Pf6N2E2_190 0.19 +1.8
242,567 - Pf6N2E2_190 0.19 +0.2
242,601 + Pf6N2E2_190 0.22 -0.6
242,713 + Pf6N2E2_190 0.33 +0.2
242,714 - Pf6N2E2_190 0.33 -1.8
242,789 + Pf6N2E2_190 0.40 +0.2
242,790 - Pf6N2E2_190 0.40 +0.1
242,790 - Pf6N2E2_190 0.40 -0.8
242,790 - Pf6N2E2_190 0.40 +0.2
242,818 + Pf6N2E2_190 0.43 +0.2
242,819 - Pf6N2E2_190 0.43 -0.8
242,819 - Pf6N2E2_190 0.43 +0.7
242,879 - Pf6N2E2_190 0.49 -0.8
242,941 + Pf6N2E2_190 0.55 -1.4
242,941 + Pf6N2E2_190 0.55 -0.9
242,941 + Pf6N2E2_190 0.55 -0.9
242,941 + Pf6N2E2_190 0.55 -0.8
242,941 + Pf6N2E2_190 0.55 -0.8
242,942 - Pf6N2E2_190 0.55 -0.8
242,942 - Pf6N2E2_190 0.55 +0.2
242,942 - Pf6N2E2_190 0.55 +0.2
242,943 + Pf6N2E2_190 0.55 -0.8
242,943 + Pf6N2E2_190 0.55 +0.2
242,943 + Pf6N2E2_190 0.55 +0.2
242,943 + Pf6N2E2_190 0.55 +0.2
242,943 + Pf6N2E2_190 0.55 -0.8
242,943 + Pf6N2E2_190 0.55 +0.2
242,943 + Pf6N2E2_190 0.55 +0.2
242,943 + Pf6N2E2_190 0.55 +0.2
242,943 + Pf6N2E2_190 0.55 +0.2
242,943 + Pf6N2E2_190 0.55 -0.8
242,943 + Pf6N2E2_190 0.55 -0.8
242,943 + Pf6N2E2_190 0.55 -0.8
242,943 + Pf6N2E2_190 0.55 +0.2
242,943 + Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 -0.5
242,944 - Pf6N2E2_190 0.55 -0.4
242,944 - Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 +0.2
242,944 - Pf6N2E2_190 0.55 -1.4
243,007 + Pf6N2E2_190 0.61 +0.2
243,046 + Pf6N2E2_190 0.65 +0.2
243,046 + Pf6N2E2_190 0.65 +0.2
243,130 + Pf6N2E2_190 0.73 -1.4
243,130 + Pf6N2E2_190 0.73 +0.2
243,130 + Pf6N2E2_190 0.73 +0.2
243,130 + Pf6N2E2_190 0.73 +0.2
243,229 + Pf6N2E2_190 0.82 +0.2
243,229 + Pf6N2E2_190 0.82 +0.2
243,229 + Pf6N2E2_190 0.82 +0.2
243,229 + Pf6N2E2_190 0.82 +0.2
243,230 - Pf6N2E2_190 0.83 +0.2
243,340 - +0.2

Or see this region's nucleotide sequence