Experiment: Ying_OrganicAcid7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1716 and Pf6N2E2_1717 are separated by 651 nucleotides Pf6N2E2_1717 and Pf6N2E2_1718 overlap by 4 nucleotides
Pf6N2E2_1716 - Phenylacetate-coenzyme A ligase (EC 6.2.1.30), at 2,023,609 to 2,024,961
_1716
Pf6N2E2_1717 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 2,025,613 to 2,027,082
_1717
Pf6N2E2_1718 - Anthranilate synthase, amidotransferase component (EC 4.1.3.27), at 2,027,079 to 2,027,663
_1718
Position (kb)
2025
2026
2027
2028 Strain fitness (log2 ratio)
-2
-1
0
1 at 2024.672 kb on + strand, within Pf6N2E2_1716 at 2024.907 kb on - strand at 2025.025 kb on - strand at 2025.027 kb on - strand at 2025.093 kb on - strand at 2025.097 kb on - strand at 2025.109 kb on - strand at 2025.347 kb on + strand at 2025.606 kb on - strand at 2025.683 kb on + strand at 2025.685 kb on + strand at 2025.914 kb on + strand, within Pf6N2E2_1717 at 2026.009 kb on + strand, within Pf6N2E2_1717 at 2026.070 kb on - strand, within Pf6N2E2_1717 at 2026.070 kb on - strand, within Pf6N2E2_1717 at 2026.070 kb on - strand, within Pf6N2E2_1717 at 2026.100 kb on - strand, within Pf6N2E2_1717 at 2026.153 kb on - strand, within Pf6N2E2_1717 at 2026.336 kb on + strand, within Pf6N2E2_1717 at 2026.429 kb on + strand, within Pf6N2E2_1717 at 2026.430 kb on - strand, within Pf6N2E2_1717 at 2026.430 kb on - strand, within Pf6N2E2_1717 at 2026.430 kb on - strand, within Pf6N2E2_1717 at 2026.430 kb on - strand, within Pf6N2E2_1717 at 2026.435 kb on + strand, within Pf6N2E2_1717 at 2026.435 kb on + strand, within Pf6N2E2_1717 at 2026.435 kb on + strand, within Pf6N2E2_1717 at 2026.435 kb on + strand, within Pf6N2E2_1717 at 2026.435 kb on + strand, within Pf6N2E2_1717 at 2026.436 kb on - strand, within Pf6N2E2_1717 at 2026.436 kb on - strand, within Pf6N2E2_1717 at 2026.436 kb on - strand, within Pf6N2E2_1717 at 2026.436 kb on - strand, within Pf6N2E2_1717 at 2026.448 kb on + strand, within Pf6N2E2_1717 at 2026.448 kb on + strand, within Pf6N2E2_1717 at 2026.448 kb on + strand, within Pf6N2E2_1717 at 2026.706 kb on - strand, within Pf6N2E2_1717 at 2026.706 kb on - strand, within Pf6N2E2_1717 at 2026.717 kb on + strand, within Pf6N2E2_1717 at 2026.718 kb on - strand, within Pf6N2E2_1717 at 2026.873 kb on + strand, within Pf6N2E2_1717 at 2026.874 kb on - strand, within Pf6N2E2_1717 at 2026.874 kb on - strand, within Pf6N2E2_1717 at 2026.956 kb on + strand at 2026.957 kb on - strand at 2026.960 kb on + strand at 2026.960 kb on + strand at 2026.961 kb on - strand at 2027.029 kb on + strand at 2027.030 kb on - strand at 2027.030 kb on - strand at 2027.030 kb on - strand at 2027.030 kb on - strand at 2027.086 kb on + strand at 2027.087 kb on - strand at 2027.134 kb on - strand at 2027.265 kb on + strand, within Pf6N2E2_1718 at 2027.539 kb on - strand, within Pf6N2E2_1718 at 2027.539 kb on - strand, within Pf6N2E2_1718 at 2027.723 kb on + strand at 2027.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3 remove 2,024,672 + Pf6N2E2_1716 0.79 -0.8 2,024,907 - +0.2 2,025,025 - -0.8 2,025,027 - -0.8 2,025,093 - +0.2 2,025,097 - +0.2 2,025,109 - -2.4 2,025,347 + -0.8 2,025,606 - +0.2 2,025,683 + -0.8 2,025,685 + +0.2 2,025,914 + Pf6N2E2_1717 0.20 -0.5 2,026,009 + Pf6N2E2_1717 0.27 +0.2 2,026,070 - Pf6N2E2_1717 0.31 +0.2 2,026,070 - Pf6N2E2_1717 0.31 -0.4 2,026,070 - Pf6N2E2_1717 0.31 +0.2 2,026,100 - Pf6N2E2_1717 0.33 -1.4 2,026,153 - Pf6N2E2_1717 0.37 -1.8 2,026,336 + Pf6N2E2_1717 0.49 +0.2 2,026,429 + Pf6N2E2_1717 0.56 -0.8 2,026,430 - Pf6N2E2_1717 0.56 +0.2 2,026,430 - Pf6N2E2_1717 0.56 -0.8 2,026,430 - Pf6N2E2_1717 0.56 +0.2 2,026,430 - Pf6N2E2_1717 0.56 -0.8 2,026,435 + Pf6N2E2_1717 0.56 +0.2 2,026,435 + Pf6N2E2_1717 0.56 -0.8 2,026,435 + Pf6N2E2_1717 0.56 +0.2 2,026,435 + Pf6N2E2_1717 0.56 +0.2 2,026,435 + Pf6N2E2_1717 0.56 +0.2 2,026,436 - Pf6N2E2_1717 0.56 +0.2 2,026,436 - Pf6N2E2_1717 0.56 +0.2 2,026,436 - Pf6N2E2_1717 0.56 +0.2 2,026,436 - Pf6N2E2_1717 0.56 -0.8 2,026,448 + Pf6N2E2_1717 0.57 -0.8 2,026,448 + Pf6N2E2_1717 0.57 -1.2 2,026,448 + Pf6N2E2_1717 0.57 -1.4 2,026,706 - Pf6N2E2_1717 0.74 +0.2 2,026,706 - Pf6N2E2_1717 0.74 +0.2 2,026,717 + Pf6N2E2_1717 0.75 -0.3 2,026,718 - Pf6N2E2_1717 0.75 -0.8 2,026,873 + Pf6N2E2_1717 0.86 +0.2 2,026,874 - Pf6N2E2_1717 0.86 +0.2 2,026,874 - Pf6N2E2_1717 0.86 +0.2 2,026,956 + +0.2 2,026,957 - +0.2 2,026,960 + +0.2 2,026,960 + -0.8 2,026,961 - +0.5 2,027,029 + +0.2 2,027,030 - +0.2 2,027,030 - +0.2 2,027,030 - +0.2 2,027,030 - +0.2 2,027,086 + -0.8 2,027,087 - -0.8 2,027,134 - -0.7 2,027,265 + Pf6N2E2_1718 0.32 +0.2 2,027,539 - Pf6N2E2_1718 0.79 -0.8 2,027,539 - Pf6N2E2_1718 0.79 -0.1 2,027,723 + +0.2 2,027,723 + -0.6
Or see this region's nucleotide sequence