Experiment: Ying_OrganicAcid7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1314 and Pf6N2E2_1315 are separated by 144 nucleotides Pf6N2E2_1315 and Pf6N2E2_1316 are separated by 252 nucleotides Pf6N2E2_1316 and Pf6N2E2_1317 are separated by 66 nucleotides
Pf6N2E2_1314 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,548,098 to 1,548,895
_1314
Pf6N2E2_1315 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68), at 1,549,040 to 1,549,462
_1315
Pf6N2E2_1316 - P-hydroxyphenylacetate hydroxylase C1:reductase component, at 1,549,715 to 1,550,656
_1316
Pf6N2E2_1317 - P-hydroxyphenylacetate hydroxylase C2:oxygenase component, at 1,550,723 to 1,551,892
_1317
Position (kb)
1549
1550
1551 Strain fitness (log2 ratio)
-2
-1
0
1 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.771 kb on - strand, within Pf6N2E2_1314 at 1548.811 kb on + strand, within Pf6N2E2_1314 at 1548.891 kb on - strand at 1549.104 kb on - strand, within Pf6N2E2_1315 at 1549.125 kb on + strand, within Pf6N2E2_1315 at 1549.497 kb on + strand at 1549.508 kb on + strand at 1549.702 kb on + strand at 1549.703 kb on - strand at 1549.859 kb on + strand, within Pf6N2E2_1316 at 1550.046 kb on + strand, within Pf6N2E2_1316 at 1550.046 kb on + strand, within Pf6N2E2_1316 at 1550.047 kb on - strand, within Pf6N2E2_1316 at 1550.047 kb on - strand, within Pf6N2E2_1316 at 1550.059 kb on - strand, within Pf6N2E2_1316 at 1550.059 kb on - strand, within Pf6N2E2_1316 at 1550.085 kb on + strand, within Pf6N2E2_1316 at 1550.085 kb on + strand, within Pf6N2E2_1316 at 1550.599 kb on + strand at 1550.600 kb on - strand at 1550.693 kb on + strand at 1550.693 kb on + strand at 1550.701 kb on + strand at 1550.707 kb on + strand at 1550.790 kb on + strand at 1550.799 kb on + strand at 1550.800 kb on - strand at 1550.925 kb on + strand, within Pf6N2E2_1317 at 1550.925 kb on + strand, within Pf6N2E2_1317 at 1550.926 kb on - strand, within Pf6N2E2_1317 at 1550.988 kb on + strand, within Pf6N2E2_1317 at 1551.199 kb on - strand, within Pf6N2E2_1317 at 1551.199 kb on - strand, within Pf6N2E2_1317 at 1551.411 kb on + strand, within Pf6N2E2_1317
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3 remove 1,548,770 + Pf6N2E2_1314 0.84 +0.1 1,548,770 + Pf6N2E2_1314 0.84 +0.1 1,548,770 + Pf6N2E2_1314 0.84 +0.1 1,548,771 - Pf6N2E2_1314 0.84 -0.5 1,548,811 + Pf6N2E2_1314 0.89 +0.1 1,548,891 - +0.1 1,549,104 - Pf6N2E2_1315 0.15 +0.1 1,549,125 + Pf6N2E2_1315 0.20 -0.5 1,549,497 + +0.1 1,549,508 + +0.1 1,549,702 + -1.9 1,549,703 - -0.3 1,549,859 + Pf6N2E2_1316 0.15 -0.9 1,550,046 + Pf6N2E2_1316 0.35 -1.9 1,550,046 + Pf6N2E2_1316 0.35 -0.9 1,550,047 - Pf6N2E2_1316 0.35 -0.9 1,550,047 - Pf6N2E2_1316 0.35 -0.9 1,550,059 - Pf6N2E2_1316 0.37 -2.4 1,550,059 - Pf6N2E2_1316 0.37 -1.5 1,550,085 + Pf6N2E2_1316 0.39 -1.9 1,550,085 + Pf6N2E2_1316 0.39 +0.1 1,550,599 + +0.1 1,550,600 - +0.1 1,550,693 + +0.1 1,550,693 + -0.9 1,550,701 + -0.3 1,550,707 + -0.7 1,550,790 + +0.1 1,550,799 + -1.5 1,550,800 - +0.1 1,550,925 + Pf6N2E2_1317 0.17 +0.1 1,550,925 + Pf6N2E2_1317 0.17 -0.9 1,550,926 - Pf6N2E2_1317 0.17 +0.1 1,550,988 + Pf6N2E2_1317 0.23 +0.1 1,551,199 - Pf6N2E2_1317 0.41 +0.1 1,551,199 - Pf6N2E2_1317 0.41 +0.1 1,551,411 + Pf6N2E2_1317 0.59 +0.1
Or see this region's nucleotide sequence