Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5999 and Pf6N2E2_6000 are separated by 26 nucleotides Pf6N2E2_6000 and Pf6N2E2_6001 are separated by 89 nucleotides Pf6N2E2_6001 and Pf6N2E2_6002 are separated by 131 nucleotides
Pf6N2E2_5999 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 6,815,468 to 6,816,745
_5999
Pf6N2E2_6000 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 6,816,772 to 6,818,916
_6000
Pf6N2E2_6001 - Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85), at 6,819,006 to 6,819,740
_6001
Pf6N2E2_6002 - Magnesium and cobalt transport protein CorA, at 6,819,872 to 6,820,843
_6002
Position (kb)
6816
6817
6818
6819 Strain fitness (log2 ratio)
-1
0
1 at 6816.100 kb on + strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.259 kb on + strand, within Pf6N2E2_5999 at 6816.260 kb on - strand, within Pf6N2E2_5999 at 6816.643 kb on + strand at 6816.868 kb on + strand at 6816.869 kb on - strand at 6816.869 kb on - strand at 6816.869 kb on - strand at 6817.056 kb on + strand, within Pf6N2E2_6000 at 6817.347 kb on + strand, within Pf6N2E2_6000 at 6817.601 kb on - strand, within Pf6N2E2_6000 at 6817.711 kb on + strand, within Pf6N2E2_6000 at 6817.712 kb on - strand, within Pf6N2E2_6000 at 6817.712 kb on - strand, within Pf6N2E2_6000 at 6817.768 kb on + strand, within Pf6N2E2_6000 at 6817.769 kb on - strand, within Pf6N2E2_6000 at 6817.769 kb on - strand, within Pf6N2E2_6000 at 6817.769 kb on - strand, within Pf6N2E2_6000 at 6817.769 kb on - strand, within Pf6N2E2_6000 at 6817.769 kb on - strand, within Pf6N2E2_6000 at 6817.800 kb on - strand, within Pf6N2E2_6000 at 6818.298 kb on + strand, within Pf6N2E2_6000 at 6818.299 kb on - strand, within Pf6N2E2_6000 at 6818.299 kb on - strand, within Pf6N2E2_6000 at 6818.525 kb on - strand, within Pf6N2E2_6000 at 6818.525 kb on - strand, within Pf6N2E2_6000 at 6818.560 kb on + strand, within Pf6N2E2_6000 at 6818.560 kb on + strand, within Pf6N2E2_6000 at 6818.560 kb on + strand, within Pf6N2E2_6000 at 6818.561 kb on - strand, within Pf6N2E2_6000 at 6818.587 kb on + strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.699 kb on + strand, within Pf6N2E2_6000 at 6818.836 kb on + strand at 6818.978 kb on - strand at 6818.998 kb on + strand at 6819.005 kb on + strand at 6819.005 kb on + strand at 6819.006 kb on - strand at 6819.006 kb on - strand at 6819.013 kb on + strand at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.136 kb on - strand, within Pf6N2E2_6001 at 6819.136 kb on - strand, within Pf6N2E2_6001 at 6819.186 kb on + strand, within Pf6N2E2_6001 at 6819.186 kb on + strand, within Pf6N2E2_6001 at 6819.252 kb on + strand, within Pf6N2E2_6001 at 6819.252 kb on + strand, within Pf6N2E2_6001 at 6819.252 kb on + strand, within Pf6N2E2_6001 at 6819.253 kb on - strand, within Pf6N2E2_6001 at 6819.523 kb on - strand, within Pf6N2E2_6001 at 6819.580 kb on - strand, within Pf6N2E2_6001 at 6819.580 kb on - strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.633 kb on - strand, within Pf6N2E2_6001 at 6819.675 kb on - strand at 6819.675 kb on - strand at 6819.873 kb on + strand at 6819.873 kb on + strand at 6819.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 6,816,100 + Pf6N2E2_5999 0.49 +0.4 6,816,258 - Pf6N2E2_5999 0.62 -1.3 6,816,258 - Pf6N2E2_5999 0.62 +0.4 6,816,258 - Pf6N2E2_5999 0.62 -0.6 6,816,258 - Pf6N2E2_5999 0.62 -1.2 6,816,259 + Pf6N2E2_5999 0.62 +0.4 6,816,260 - Pf6N2E2_5999 0.62 +0.4 6,816,643 + -0.6 6,816,868 + -0.1 6,816,869 - -1.7 6,816,869 - +0.4 6,816,869 - -0.9 6,817,056 + Pf6N2E2_6000 0.13 -0.4 6,817,347 + Pf6N2E2_6000 0.27 +0.0 6,817,601 - Pf6N2E2_6000 0.39 +0.4 6,817,711 + Pf6N2E2_6000 0.44 -0.6 6,817,712 - Pf6N2E2_6000 0.44 -0.6 6,817,712 - Pf6N2E2_6000 0.44 -0.6 6,817,768 + Pf6N2E2_6000 0.46 +0.4 6,817,769 - Pf6N2E2_6000 0.46 +0.4 6,817,769 - Pf6N2E2_6000 0.46 -1.2 6,817,769 - Pf6N2E2_6000 0.46 -1.4 6,817,769 - Pf6N2E2_6000 0.46 -0.2 6,817,769 - Pf6N2E2_6000 0.46 +0.1 6,817,800 - Pf6N2E2_6000 0.48 -1.6 6,818,298 + Pf6N2E2_6000 0.71 -1.0 6,818,299 - Pf6N2E2_6000 0.71 +0.4 6,818,299 - Pf6N2E2_6000 0.71 +0.4 6,818,525 - Pf6N2E2_6000 0.82 +0.4 6,818,525 - Pf6N2E2_6000 0.82 -0.9 6,818,560 + Pf6N2E2_6000 0.83 -0.6 6,818,560 + Pf6N2E2_6000 0.83 -0.7 6,818,560 + Pf6N2E2_6000 0.83 -0.6 6,818,561 - Pf6N2E2_6000 0.83 +0.4 6,818,587 + Pf6N2E2_6000 0.85 -0.2 6,818,588 - Pf6N2E2_6000 0.85 -0.6 6,818,588 - Pf6N2E2_6000 0.85 +0.4 6,818,588 - Pf6N2E2_6000 0.85 -0.6 6,818,588 - Pf6N2E2_6000 0.85 -0.5 6,818,699 + Pf6N2E2_6000 0.90 -0.5 6,818,836 + -0.6 6,818,978 - -0.4 6,818,998 + +0.4 6,819,005 + +0.4 6,819,005 + +0.4 6,819,006 - +0.4 6,819,006 - -1.2 6,819,013 + +0.4 6,819,135 + Pf6N2E2_6001 0.18 -1.2 6,819,135 + Pf6N2E2_6001 0.18 -0.5 6,819,135 + Pf6N2E2_6001 0.18 +0.4 6,819,135 + Pf6N2E2_6001 0.18 -1.2 6,819,136 - Pf6N2E2_6001 0.18 +0.4 6,819,136 - Pf6N2E2_6001 0.18 +0.4 6,819,186 + Pf6N2E2_6001 0.24 -0.6 6,819,186 + Pf6N2E2_6001 0.24 +0.4 6,819,252 + Pf6N2E2_6001 0.33 -0.6 6,819,252 + Pf6N2E2_6001 0.33 +0.4 6,819,252 + Pf6N2E2_6001 0.33 +0.4 6,819,253 - Pf6N2E2_6001 0.34 +0.4 6,819,523 - Pf6N2E2_6001 0.70 +0.4 6,819,580 - Pf6N2E2_6001 0.78 -1.2 6,819,580 - Pf6N2E2_6001 0.78 +0.4 6,819,630 + Pf6N2E2_6001 0.85 -0.2 6,819,630 + Pf6N2E2_6001 0.85 -1.2 6,819,630 + Pf6N2E2_6001 0.85 -0.4 6,819,630 + Pf6N2E2_6001 0.85 -0.6 6,819,630 + Pf6N2E2_6001 0.85 +0.4 6,819,630 + Pf6N2E2_6001 0.85 +0.4 6,819,630 + Pf6N2E2_6001 0.85 -0.6 6,819,630 + Pf6N2E2_6001 0.85 -1.1 6,819,631 - Pf6N2E2_6001 0.85 -0.6 6,819,631 - Pf6N2E2_6001 0.85 +0.4 6,819,631 - Pf6N2E2_6001 0.85 +0.4 6,819,631 - Pf6N2E2_6001 0.85 -0.3 6,819,633 - Pf6N2E2_6001 0.85 -1.2 6,819,675 - +0.4 6,819,675 - -1.2 6,819,873 + +0.4 6,819,873 + +0.4 6,819,874 - -0.6
Or see this region's nucleotide sequence