Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5244 and Pf6N2E2_5245 are separated by 205 nucleotides Pf6N2E2_5245 and Pf6N2E2_5246 are separated by 63 nucleotides Pf6N2E2_5246 and Pf6N2E2_5247 are separated by 257 nucleotides Pf6N2E2_5247 and Pf6N2E2_5248 overlap by 12 nucleotides Pf6N2E2_5248 and Pf6N2E2_5249 are separated by 210 nucleotides
Pf6N2E2_5244 - FIG00954931: hypothetical protein, at 6,041,125 to 6,041,358
_5244
Pf6N2E2_5245 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 6,041,564 to 6,042,148
_5245
Pf6N2E2_5246 - FIG00955938: hypothetical protein, at 6,042,212 to 6,042,418
_5246
Pf6N2E2_5247 - hypothetical protein, at 6,042,676 to 6,042,792
_5247
Pf6N2E2_5248 - FIG00955916: hypothetical protein, at 6,042,781 to 6,043,062
_5248
Pf6N2E2_5249 - Glycerol-3-phosphate O-acyltransferase, at 6,043,273 to 6,044,436
_5249
Position (kb)
6042
6043 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6041.346 kb on + strand at 6041.346 kb on + strand at 6041.346 kb on + strand at 6041.346 kb on + strand at 6041.405 kb on + strand at 6041.420 kb on + strand at 6041.420 kb on + strand at 6041.420 kb on + strand at 6041.420 kb on + strand at 6041.420 kb on + strand at 6041.421 kb on - strand at 6041.421 kb on - strand at 6041.421 kb on - strand at 6041.421 kb on - strand at 6041.481 kb on + strand at 6041.498 kb on + strand at 6041.498 kb on + strand at 6041.498 kb on + strand at 6041.499 kb on - strand at 6041.523 kb on - strand at 6041.912 kb on + strand, within Pf6N2E2_5245 at 6041.912 kb on + strand, within Pf6N2E2_5245 at 6041.912 kb on + strand, within Pf6N2E2_5245 at 6041.912 kb on + strand, within Pf6N2E2_5245 at 6041.913 kb on - strand, within Pf6N2E2_5245 at 6041.913 kb on - strand, within Pf6N2E2_5245 at 6041.913 kb on - strand, within Pf6N2E2_5245 at 6041.936 kb on + strand, within Pf6N2E2_5245 at 6041.936 kb on + strand, within Pf6N2E2_5245 at 6041.936 kb on + strand, within Pf6N2E2_5245 at 6041.936 kb on + strand, within Pf6N2E2_5245 at 6041.936 kb on + strand, within Pf6N2E2_5245 at 6041.936 kb on + strand, within Pf6N2E2_5245 at 6041.936 kb on + strand, within Pf6N2E2_5245 at 6041.936 kb on + strand, within Pf6N2E2_5245 at 6041.936 kb on + strand, within Pf6N2E2_5245 at 6041.937 kb on - strand, within Pf6N2E2_5245 at 6041.937 kb on - strand, within Pf6N2E2_5245 at 6041.937 kb on - strand, within Pf6N2E2_5245 at 6041.937 kb on - strand, within Pf6N2E2_5245 at 6041.937 kb on - strand, within Pf6N2E2_5245 at 6041.937 kb on - strand, within Pf6N2E2_5245 at 6041.937 kb on - strand, within Pf6N2E2_5245 at 6041.937 kb on - strand, within Pf6N2E2_5245 at 6041.937 kb on - strand, within Pf6N2E2_5245 at 6042.146 kb on + strand at 6042.147 kb on - strand at 6042.147 kb on - strand at 6042.147 kb on - strand at 6042.147 kb on - strand at 6042.150 kb on + strand at 6042.150 kb on + strand at 6042.150 kb on + strand at 6042.151 kb on - strand at 6042.151 kb on - strand at 6042.151 kb on - strand at 6042.151 kb on - strand at 6042.151 kb on - strand at 6042.229 kb on - strand at 6042.306 kb on + strand, within Pf6N2E2_5246 at 6042.307 kb on - strand, within Pf6N2E2_5246 at 6042.307 kb on - strand, within Pf6N2E2_5246 at 6042.307 kb on - strand, within Pf6N2E2_5246 at 6042.307 kb on - strand, within Pf6N2E2_5246 at 6042.307 kb on - strand, within Pf6N2E2_5246 at 6042.307 kb on - strand, within Pf6N2E2_5246 at 6042.387 kb on + strand, within Pf6N2E2_5246 at 6042.387 kb on + strand, within Pf6N2E2_5246 at 6042.458 kb on + strand at 6042.490 kb on + strand at 6042.562 kb on + strand at 6042.563 kb on - strand at 6042.738 kb on - strand, within Pf6N2E2_5247 at 6043.050 kb on + strand at 6043.082 kb on + strand at 6043.082 kb on + strand at 6043.083 kb on - strand at 6043.083 kb on - strand at 6043.083 kb on - strand at 6043.083 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 6,041,346 + +1.4 6,041,346 + -0.6 6,041,346 + +0.4 6,041,346 + -0.6 6,041,405 + -0.6 6,041,420 + -1.5 6,041,420 + -0.6 6,041,420 + -0.5 6,041,420 + -0.6 6,041,420 + -1.3 6,041,421 - +0.4 6,041,421 - +0.4 6,041,421 - +0.4 6,041,421 - -0.3 6,041,481 + +0.4 6,041,498 + -0.6 6,041,498 + +0.6 6,041,498 + +0.4 6,041,499 - -1.1 6,041,523 - +0.4 6,041,912 + Pf6N2E2_5245 0.59 -0.6 6,041,912 + Pf6N2E2_5245 0.59 +0.4 6,041,912 + Pf6N2E2_5245 0.59 +0.4 6,041,912 + Pf6N2E2_5245 0.59 -1.1 6,041,913 - Pf6N2E2_5245 0.60 +0.4 6,041,913 - Pf6N2E2_5245 0.60 +0.4 6,041,913 - Pf6N2E2_5245 0.60 +0.4 6,041,936 + Pf6N2E2_5245 0.64 +0.4 6,041,936 + Pf6N2E2_5245 0.64 +0.4 6,041,936 + Pf6N2E2_5245 0.64 +0.4 6,041,936 + Pf6N2E2_5245 0.64 -1.1 6,041,936 + Pf6N2E2_5245 0.64 +0.4 6,041,936 + Pf6N2E2_5245 0.64 -0.6 6,041,936 + Pf6N2E2_5245 0.64 +0.4 6,041,936 + Pf6N2E2_5245 0.64 +0.4 6,041,936 + Pf6N2E2_5245 0.64 +0.2 6,041,937 - Pf6N2E2_5245 0.64 -0.6 6,041,937 - Pf6N2E2_5245 0.64 -1.3 6,041,937 - Pf6N2E2_5245 0.64 -1.1 6,041,937 - Pf6N2E2_5245 0.64 +0.4 6,041,937 - Pf6N2E2_5245 0.64 -0.5 6,041,937 - Pf6N2E2_5245 0.64 +0.4 6,041,937 - Pf6N2E2_5245 0.64 +0.4 6,041,937 - Pf6N2E2_5245 0.64 +0.4 6,041,937 - Pf6N2E2_5245 0.64 +0.4 6,042,146 + +0.4 6,042,147 - +0.4 6,042,147 - -0.6 6,042,147 - -1.6 6,042,147 - -1.6 6,042,150 + +0.0 6,042,150 + -0.7 6,042,150 + -0.6 6,042,151 - -0.6 6,042,151 - -0.6 6,042,151 - +0.4 6,042,151 - -1.3 6,042,151 - -2.9 6,042,229 - -0.6 6,042,306 + Pf6N2E2_5246 0.45 -0.5 6,042,307 - Pf6N2E2_5246 0.46 -0.1 6,042,307 - Pf6N2E2_5246 0.46 +0.4 6,042,307 - Pf6N2E2_5246 0.46 +0.4 6,042,307 - Pf6N2E2_5246 0.46 +0.4 6,042,307 - Pf6N2E2_5246 0.46 +0.4 6,042,307 - Pf6N2E2_5246 0.46 +2.1 6,042,387 + Pf6N2E2_5246 0.85 +0.4 6,042,387 + Pf6N2E2_5246 0.85 -0.1 6,042,458 + +0.4 6,042,490 + -1.6 6,042,562 + -0.6 6,042,563 - +0.4 6,042,738 - Pf6N2E2_5247 0.53 -0.6 6,043,050 + +0.4 6,043,082 + -1.0 6,043,082 + -2.1 6,043,083 - -1.9 6,043,083 - +0.4 6,043,083 - +0.4 6,043,083 - +0.4
Or see this region's nucleotide sequence