Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5153 and Pf6N2E2_5154 are separated by 275 nucleotides Pf6N2E2_5154 and Pf6N2E2_5155 are separated by 3 nucleotides Pf6N2E2_5155 and Pf6N2E2_5156 are separated by 718 nucleotides
Pf6N2E2_5153 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 5,940,125 to 5,940,925
_5153
Pf6N2E2_5154 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 5,941,201 to 5,941,686
_5154
Pf6N2E2_5155 - Poly(A) polymerase (EC 2.7.7.19), at 5,941,690 to 5,943,087
_5155
Pf6N2E2_5156 - Two-component sensor CbrB: intrcellular carbon:nitrogen balance, at 5,943,806 to 5,945,239
_5156
Position (kb)
5941
5942
5943
5944 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5940.726 kb on + strand, within Pf6N2E2_5153 at 5940.727 kb on - strand, within Pf6N2E2_5153 at 5940.853 kb on - strand at 5940.853 kb on - strand at 5940.853 kb on - strand at 5940.914 kb on - strand at 5940.916 kb on + strand at 5940.916 kb on + strand at 5940.916 kb on + strand at 5940.916 kb on + strand at 5940.917 kb on - strand at 5940.917 kb on - strand at 5940.917 kb on - strand at 5940.917 kb on - strand at 5940.940 kb on + strand at 5940.941 kb on - strand at 5940.955 kb on + strand at 5940.956 kb on - strand at 5940.965 kb on - strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.988 kb on - strand at 5941.002 kb on + strand at 5941.002 kb on + strand at 5941.002 kb on + strand at 5941.002 kb on + strand at 5941.003 kb on - strand at 5941.003 kb on - strand at 5941.003 kb on - strand at 5941.003 kb on - strand at 5941.017 kb on + strand at 5941.018 kb on - strand at 5941.018 kb on - strand at 5941.018 kb on - strand at 5941.069 kb on - strand at 5941.069 kb on - strand at 5941.069 kb on - strand at 5941.070 kb on + strand at 5941.070 kb on + strand at 5941.070 kb on + strand at 5941.071 kb on - strand at 5941.071 kb on - strand at 5941.071 kb on - strand at 5941.071 kb on - strand at 5941.130 kb on + strand at 5941.206 kb on + strand at 5941.674 kb on - strand at 5941.674 kb on - strand at 5942.235 kb on - strand, within Pf6N2E2_5155 at 5942.462 kb on - strand, within Pf6N2E2_5155 at 5942.592 kb on - strand, within Pf6N2E2_5155 at 5942.595 kb on - strand, within Pf6N2E2_5155 at 5942.830 kb on + strand, within Pf6N2E2_5155 at 5942.919 kb on - strand, within Pf6N2E2_5155 at 5942.919 kb on - strand, within Pf6N2E2_5155 at 5943.110 kb on - strand at 5943.154 kb on + strand at 5943.154 kb on + strand at 5943.155 kb on - strand at 5943.161 kb on + strand at 5943.161 kb on + strand at 5943.223 kb on + strand at 5943.224 kb on - strand at 5943.282 kb on + strand at 5943.283 kb on - strand at 5943.287 kb on - strand at 5943.302 kb on + strand at 5943.306 kb on - strand at 5943.468 kb on - strand at 5943.468 kb on - strand at 5943.503 kb on + strand at 5943.504 kb on - strand at 5943.504 kb on - strand at 5943.504 kb on - strand at 5943.504 kb on - strand at 5943.529 kb on + strand at 5943.530 kb on - strand at 5943.557 kb on - strand at 5943.581 kb on - strand at 5943.586 kb on + strand at 5943.608 kb on + strand at 5943.619 kb on + strand at 5943.628 kb on + strand at 5943.628 kb on + strand at 5943.629 kb on - strand at 5943.686 kb on + strand at 5943.686 kb on + strand at 5943.687 kb on - strand at 5943.688 kb on + strand at 5943.689 kb on - strand at 5943.732 kb on + strand at 5943.733 kb on - strand at 5943.733 kb on - strand at 5943.750 kb on - strand at 5943.768 kb on - strand at 5943.768 kb on - strand at 5943.955 kb on - strand, within Pf6N2E2_5156
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 5,940,726 + Pf6N2E2_5153 0.75 +0.5 5,940,727 - Pf6N2E2_5153 0.75 -0.5 5,940,853 - +0.5 5,940,853 - +0.5 5,940,853 - +0.5 5,940,914 - +0.5 5,940,916 + -0.5 5,940,916 + +0.5 5,940,916 + +0.5 5,940,916 + -1.1 5,940,917 - -1.5 5,940,917 - -1.1 5,940,917 - -1.5 5,940,917 - +0.5 5,940,940 + -1.1 5,940,941 - -1.1 5,940,955 + +0.5 5,940,956 - -1.1 5,940,965 - -1.1 5,940,985 + +0.5 5,940,985 + +0.5 5,940,985 + +0.5 5,940,985 + +0.5 5,940,985 + -0.9 5,940,985 + -0.5 5,940,985 + -1.1 5,940,986 - +0.5 5,940,986 - +0.5 5,940,986 - +0.5 5,940,986 - +0.5 5,940,986 - -1.5 5,940,986 - -1.1 5,940,986 - -0.5 5,940,986 - +0.5 5,940,986 - +0.5 5,940,986 - -0.5 5,940,986 - +0.5 5,940,986 - -1.5 5,940,988 - -0.3 5,941,002 + -0.5 5,941,002 + -0.5 5,941,002 + +0.5 5,941,002 + -0.5 5,941,003 - +0.5 5,941,003 - +0.5 5,941,003 - +1.0 5,941,003 - -0.5 5,941,017 + +0.5 5,941,018 - -0.5 5,941,018 - +0.5 5,941,018 - -1.9 5,941,069 - -0.5 5,941,069 - +0.5 5,941,069 - -1.1 5,941,070 + -1.1 5,941,070 + -0.5 5,941,070 + -0.5 5,941,071 - -0.5 5,941,071 - +0.5 5,941,071 - -2.1 5,941,071 - +0.5 5,941,130 + -0.5 5,941,206 + -0.2 5,941,674 - -0.5 5,941,674 - -2.1 5,942,235 - Pf6N2E2_5155 0.39 +0.0 5,942,462 - Pf6N2E2_5155 0.55 +0.5 5,942,592 - Pf6N2E2_5155 0.65 -1.9 5,942,595 - Pf6N2E2_5155 0.65 -0.5 5,942,830 + Pf6N2E2_5155 0.82 -1.5 5,942,919 - Pf6N2E2_5155 0.88 -2.7 5,942,919 - Pf6N2E2_5155 0.88 +0.5 5,943,110 - +0.5 5,943,154 + -1.7 5,943,154 + -2.2 5,943,155 - +0.5 5,943,161 + +0.5 5,943,161 + +0.5 5,943,223 + +0.5 5,943,224 - -0.5 5,943,282 + -0.5 5,943,283 - -0.3 5,943,287 - +0.5 5,943,302 + -1.1 5,943,306 - -1.5 5,943,468 - -0.5 5,943,468 - +0.5 5,943,503 + -0.5 5,943,504 - -1.1 5,943,504 - -0.5 5,943,504 - -1.1 5,943,504 - -1.4 5,943,529 + -0.5 5,943,530 - +0.5 5,943,557 - +0.5 5,943,581 - -1.7 5,943,586 + +0.5 5,943,608 + -0.5 5,943,619 + -1.6 5,943,628 + -1.5 5,943,628 + +0.5 5,943,629 - -0.4 5,943,686 + -0.5 5,943,686 + +0.5 5,943,687 - +0.5 5,943,688 + -3.0 5,943,689 - -1.9 5,943,732 + +0.5 5,943,733 - +0.5 5,943,733 - +0.5 5,943,750 - +0.5 5,943,768 - +0.5 5,943,768 - -1.5 5,943,955 - Pf6N2E2_5156 0.10 -0.5
Or see this region's nucleotide sequence