Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5085 and Pf6N2E2_5086 are separated by 401 nucleotides Pf6N2E2_5086 and Pf6N2E2_5087 are separated by 230 nucleotides Pf6N2E2_5087 and Pf6N2E2_5088 are separated by 70 nucleotides Pf6N2E2_5088 and Pf6N2E2_5089 are separated by 114 nucleotides
Pf6N2E2_5085 - Lanthionine biosynthesis protein LanM, at 5,866,264 to 5,869,347
_5085
Pf6N2E2_5086 - hypothetical protein, at 5,869,749 to 5,869,892
_5086
Pf6N2E2_5087 - Transcription elongation factor GreB-related protein, at 5,870,123 to 5,870,614
_5087
Pf6N2E2_5088 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,870,685 to 5,871,041
_5088
Pf6N2E2_5089 - hypothetical protein, at 5,871,156 to 5,871,554
_5089
Position (kb)
5870
5871 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5869.131 kb on + strand at 5869.188 kb on + strand at 5869.189 kb on - strand at 5869.190 kb on + strand at 5869.190 kb on + strand at 5869.190 kb on + strand at 5869.191 kb on - strand at 5869.301 kb on + strand at 5869.308 kb on - strand at 5869.353 kb on - strand at 5869.389 kb on + strand at 5869.398 kb on - strand at 5869.398 kb on - strand at 5869.398 kb on - strand at 5869.433 kb on + strand at 5869.459 kb on - strand at 5869.669 kb on + strand at 5869.670 kb on - strand at 5869.709 kb on + strand at 5869.876 kb on - strand, within Pf6N2E2_5086 at 5869.992 kb on - strand at 5870.012 kb on - strand at 5870.038 kb on - strand at 5870.096 kb on - strand at 5870.096 kb on - strand at 5870.529 kb on + strand, within Pf6N2E2_5087 at 5870.529 kb on + strand, within Pf6N2E2_5087 at 5870.529 kb on + strand, within Pf6N2E2_5087 at 5870.529 kb on + strand, within Pf6N2E2_5087 at 5870.530 kb on - strand, within Pf6N2E2_5087 at 5870.530 kb on - strand, within Pf6N2E2_5087 at 5870.530 kb on - strand, within Pf6N2E2_5087 at 5870.628 kb on - strand at 5870.686 kb on + strand at 5870.687 kb on - strand at 5870.857 kb on + strand, within Pf6N2E2_5088 at 5870.858 kb on - strand, within Pf6N2E2_5088 at 5870.933 kb on - strand, within Pf6N2E2_5088 at 5871.071 kb on - strand at 5871.071 kb on - strand at 5871.083 kb on + strand at 5871.083 kb on + strand at 5871.084 kb on - strand at 5871.084 kb on - strand at 5871.097 kb on + strand at 5871.097 kb on + strand at 5871.097 kb on + strand at 5871.098 kb on - strand at 5871.141 kb on + strand at 5871.141 kb on + strand at 5871.142 kb on - strand at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.310 kb on + strand, within Pf6N2E2_5089 at 5871.310 kb on + strand, within Pf6N2E2_5089 at 5871.310 kb on + strand, within Pf6N2E2_5089 at 5871.311 kb on - strand, within Pf6N2E2_5089 at 5871.328 kb on + strand, within Pf6N2E2_5089 at 5871.472 kb on + strand, within Pf6N2E2_5089 at 5871.487 kb on + strand, within Pf6N2E2_5089 at 5871.488 kb on - strand, within Pf6N2E2_5089 at 5871.511 kb on + strand, within Pf6N2E2_5089 at 5871.512 kb on - strand, within Pf6N2E2_5089 at 5871.520 kb on + strand at 5871.520 kb on + strand at 5871.521 kb on - strand at 5871.521 kb on - strand at 5871.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 5,869,131 + +0.4 5,869,188 + -0.6 5,869,189 - -1.1 5,869,190 + -0.7 5,869,190 + +0.4 5,869,190 + +0.4 5,869,191 - -1.0 5,869,301 + +0.4 5,869,308 - +0.4 5,869,353 - +0.4 5,869,389 + +0.4 5,869,398 - +0.4 5,869,398 - -0.6 5,869,398 - +0.4 5,869,433 + +0.3 5,869,459 - -0.6 5,869,669 + -0.6 5,869,670 - -0.6 5,869,709 + +0.4 5,869,876 - Pf6N2E2_5086 0.88 +2.0 5,869,992 - +1.0 5,870,012 - -1.1 5,870,038 - -0.6 5,870,096 - +0.0 5,870,096 - +0.4 5,870,529 + Pf6N2E2_5087 0.83 -1.1 5,870,529 + Pf6N2E2_5087 0.83 -0.6 5,870,529 + Pf6N2E2_5087 0.83 -0.6 5,870,529 + Pf6N2E2_5087 0.83 +0.4 5,870,530 - Pf6N2E2_5087 0.83 -0.6 5,870,530 - Pf6N2E2_5087 0.83 +0.4 5,870,530 - Pf6N2E2_5087 0.83 +0.4 5,870,628 - -0.4 5,870,686 + -1.1 5,870,687 - -0.3 5,870,857 + Pf6N2E2_5088 0.48 -0.5 5,870,858 - Pf6N2E2_5088 0.48 -0.6 5,870,933 - Pf6N2E2_5088 0.69 +0.4 5,871,071 - -0.6 5,871,071 - +0.4 5,871,083 + -0.8 5,871,083 + -0.6 5,871,084 - -0.4 5,871,084 - -0.2 5,871,097 + -0.6 5,871,097 + +0.4 5,871,097 + -2.3 5,871,098 - -1.0 5,871,141 + -0.6 5,871,141 + +0.4 5,871,142 - +0.4 5,871,238 + Pf6N2E2_5089 0.21 -0.3 5,871,238 + Pf6N2E2_5089 0.21 -0.5 5,871,238 + Pf6N2E2_5089 0.21 -0.6 5,871,238 + Pf6N2E2_5089 0.21 -0.3 5,871,238 + Pf6N2E2_5089 0.21 -0.4 5,871,238 + Pf6N2E2_5089 0.21 -0.4 5,871,238 + Pf6N2E2_5089 0.21 -0.4 5,871,238 + Pf6N2E2_5089 0.21 -0.3 5,871,239 - Pf6N2E2_5089 0.21 -0.4 5,871,239 - Pf6N2E2_5089 0.21 -0.5 5,871,239 - Pf6N2E2_5089 0.21 -0.4 5,871,239 - Pf6N2E2_5089 0.21 -0.5 5,871,239 - Pf6N2E2_5089 0.21 -0.4 5,871,239 - Pf6N2E2_5089 0.21 -0.3 5,871,239 - Pf6N2E2_5089 0.21 -0.4 5,871,239 - Pf6N2E2_5089 0.21 -0.3 5,871,239 - Pf6N2E2_5089 0.21 -0.4 5,871,239 - Pf6N2E2_5089 0.21 -0.4 5,871,239 - Pf6N2E2_5089 0.21 -0.5 5,871,239 - Pf6N2E2_5089 0.21 -0.5 5,871,247 + Pf6N2E2_5089 0.23 -1.0 5,871,247 + Pf6N2E2_5089 0.23 -0.4 5,871,247 + Pf6N2E2_5089 0.23 -0.4 5,871,247 + Pf6N2E2_5089 0.23 -0.4 5,871,247 + Pf6N2E2_5089 0.23 -0.4 5,871,247 + Pf6N2E2_5089 0.23 -0.3 5,871,248 - Pf6N2E2_5089 0.23 -0.4 5,871,248 - Pf6N2E2_5089 0.23 -0.3 5,871,248 - Pf6N2E2_5089 0.23 -0.4 5,871,248 - Pf6N2E2_5089 0.23 -0.4 5,871,248 - Pf6N2E2_5089 0.23 -0.3 5,871,248 - Pf6N2E2_5089 0.23 -0.5 5,871,248 - Pf6N2E2_5089 0.23 -0.3 5,871,248 - Pf6N2E2_5089 0.23 -0.4 5,871,248 - Pf6N2E2_5089 0.23 -0.4 5,871,310 + Pf6N2E2_5089 0.39 -0.4 5,871,310 + Pf6N2E2_5089 0.39 -0.6 5,871,310 + Pf6N2E2_5089 0.39 -0.3 5,871,311 - Pf6N2E2_5089 0.39 -0.3 5,871,328 + Pf6N2E2_5089 0.43 -0.4 5,871,472 + Pf6N2E2_5089 0.79 -0.6 5,871,487 + Pf6N2E2_5089 0.83 -0.9 5,871,488 - Pf6N2E2_5089 0.83 -0.4 5,871,511 + Pf6N2E2_5089 0.89 -0.3 5,871,512 - Pf6N2E2_5089 0.89 -0.4 5,871,520 + -0.5 5,871,520 + -0.4 5,871,521 - -0.5 5,871,521 - -0.3 5,871,581 - -0.5
Or see this region's nucleotide sequence