Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4988 and Pf6N2E2_4989 overlap by 4 nucleotides Pf6N2E2_4989 and Pf6N2E2_4990 are separated by 10 nucleotides Pf6N2E2_4990 and Pf6N2E2_4991 are separated by 66 nucleotides
Pf6N2E2_4988 - Antiholin-like protein LrgA, at 5,755,132 to 5,755,497
_4988
Pf6N2E2_4989 - LrgA-associated membrane protein LrgB, at 5,755,494 to 5,756,210
_4989
Pf6N2E2_4990 - Uncharacterized protein, similar to the N-terminal domain of Lon protease, at 5,756,221 to 5,756,811
_4990
Pf6N2E2_4991 - DedA protein, at 5,756,878 to 5,758,194
_4991
Position (kb)
5756
5757 Strain fitness (log2 ratio)
-2
-1
0
1 at 5755.312 kb on + strand, within Pf6N2E2_4988 at 5755.357 kb on + strand, within Pf6N2E2_4988 at 5755.357 kb on + strand, within Pf6N2E2_4988 at 5755.358 kb on - strand, within Pf6N2E2_4988 at 5755.358 kb on - strand, within Pf6N2E2_4988 at 5755.358 kb on - strand, within Pf6N2E2_4988 at 5755.358 kb on - strand, within Pf6N2E2_4988 at 5755.358 kb on - strand, within Pf6N2E2_4988 at 5755.573 kb on - strand, within Pf6N2E2_4989 at 5755.759 kb on - strand, within Pf6N2E2_4989 at 5755.759 kb on - strand, within Pf6N2E2_4989 at 5755.805 kb on + strand, within Pf6N2E2_4989 at 5755.805 kb on + strand, within Pf6N2E2_4989 at 5755.805 kb on + strand, within Pf6N2E2_4989 at 5755.806 kb on - strand, within Pf6N2E2_4989 at 5755.806 kb on - strand, within Pf6N2E2_4989 at 5756.093 kb on + strand, within Pf6N2E2_4989 at 5756.208 kb on + strand at 5756.221 kb on - strand at 5756.395 kb on + strand, within Pf6N2E2_4990 at 5756.487 kb on + strand, within Pf6N2E2_4990 at 5756.693 kb on - strand, within Pf6N2E2_4990 at 5756.693 kb on - strand, within Pf6N2E2_4990 at 5756.822 kb on - strand at 5756.833 kb on + strand at 5756.882 kb on + strand at 5756.882 kb on + strand at 5756.882 kb on + strand at 5756.883 kb on - strand at 5757.089 kb on + strand, within Pf6N2E2_4991 at 5757.239 kb on + strand, within Pf6N2E2_4991 at 5757.240 kb on - strand, within Pf6N2E2_4991 at 5757.240 kb on - strand, within Pf6N2E2_4991 at 5757.723 kb on - strand, within Pf6N2E2_4991 at 5757.723 kb on - strand, within Pf6N2E2_4991
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 5,755,312 + Pf6N2E2_4988 0.49 -2.1 5,755,357 + Pf6N2E2_4988 0.61 +1.1 5,755,357 + Pf6N2E2_4988 0.61 +0.4 5,755,358 - Pf6N2E2_4988 0.62 -0.5 5,755,358 - Pf6N2E2_4988 0.62 -0.6 5,755,358 - Pf6N2E2_4988 0.62 -0.6 5,755,358 - Pf6N2E2_4988 0.62 -0.6 5,755,358 - Pf6N2E2_4988 0.62 -0.7 5,755,573 - Pf6N2E2_4989 0.11 -1.2 5,755,759 - Pf6N2E2_4989 0.37 -0.6 5,755,759 - Pf6N2E2_4989 0.37 -0.4 5,755,805 + Pf6N2E2_4989 0.43 -2.1 5,755,805 + Pf6N2E2_4989 0.43 -0.6 5,755,805 + Pf6N2E2_4989 0.43 -0.6 5,755,806 - Pf6N2E2_4989 0.44 -1.9 5,755,806 - Pf6N2E2_4989 0.44 -1.1 5,756,093 + Pf6N2E2_4989 0.84 -0.3 5,756,208 + +0.6 5,756,221 - +0.5 5,756,395 + Pf6N2E2_4990 0.29 -0.5 5,756,487 + Pf6N2E2_4990 0.45 +0.5 5,756,693 - Pf6N2E2_4990 0.80 -0.5 5,756,693 - Pf6N2E2_4990 0.80 -1.1 5,756,822 - -1.1 5,756,833 + -1.5 5,756,882 + -0.8 5,756,882 + -0.5 5,756,882 + +0.5 5,756,883 - -1.1 5,757,089 + Pf6N2E2_4991 0.16 +0.4 5,757,239 + Pf6N2E2_4991 0.27 -1.9 5,757,240 - Pf6N2E2_4991 0.27 -0.6 5,757,240 - Pf6N2E2_4991 0.27 -1.9 5,757,723 - Pf6N2E2_4991 0.64 +0.4 5,757,723 - Pf6N2E2_4991 0.64 -0.6
Or see this region's nucleotide sequence