Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4834 and Pf6N2E2_4835 are separated by 35 nucleotides Pf6N2E2_4835 and Pf6N2E2_4836 are separated by 135 nucleotides
Pf6N2E2_4834 - 2-nitropropane dioxygenase (EC 1.13.11.32), at 5,586,830 to 5,587,798
_4834
Pf6N2E2_4835 - Protoporphyrinogen IX oxidase, novel form, HemJ (EC 1.3.-.-), at 5,587,834 to 5,588,265
_4835
Pf6N2E2_4836 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 5,588,401 to 5,589,435
_4836
Position (kb)
5587
5588
5589 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5586.882 kb on + strand at 5586.883 kb on - strand at 5586.963 kb on - strand, within Pf6N2E2_4834 at 5586.963 kb on - strand, within Pf6N2E2_4834 at 5587.104 kb on - strand, within Pf6N2E2_4834 at 5587.114 kb on + strand, within Pf6N2E2_4834 at 5587.114 kb on + strand, within Pf6N2E2_4834 at 5587.115 kb on - strand, within Pf6N2E2_4834 at 5587.115 kb on - strand, within Pf6N2E2_4834 at 5587.115 kb on - strand, within Pf6N2E2_4834 at 5587.115 kb on - strand, within Pf6N2E2_4834 at 5587.157 kb on - strand, within Pf6N2E2_4834 at 5587.157 kb on - strand, within Pf6N2E2_4834 at 5587.157 kb on - strand, within Pf6N2E2_4834 at 5587.157 kb on - strand, within Pf6N2E2_4834 at 5587.157 kb on - strand, within Pf6N2E2_4834 at 5587.158 kb on + strand, within Pf6N2E2_4834 at 5587.158 kb on + strand, within Pf6N2E2_4834 at 5587.158 kb on + strand, within Pf6N2E2_4834 at 5587.159 kb on - strand, within Pf6N2E2_4834 at 5587.210 kb on - strand, within Pf6N2E2_4834 at 5587.210 kb on - strand, within Pf6N2E2_4834 at 5587.210 kb on - strand, within Pf6N2E2_4834 at 5587.280 kb on + strand, within Pf6N2E2_4834 at 5587.280 kb on + strand, within Pf6N2E2_4834 at 5587.281 kb on - strand, within Pf6N2E2_4834 at 5587.281 kb on - strand, within Pf6N2E2_4834 at 5587.281 kb on - strand, within Pf6N2E2_4834 at 5587.281 kb on - strand, within Pf6N2E2_4834 at 5587.281 kb on - strand, within Pf6N2E2_4834 at 5587.289 kb on - strand, within Pf6N2E2_4834 at 5587.449 kb on + strand, within Pf6N2E2_4834 at 5587.449 kb on + strand, within Pf6N2E2_4834 at 5587.450 kb on - strand, within Pf6N2E2_4834 at 5587.466 kb on + strand, within Pf6N2E2_4834 at 5587.466 kb on + strand, within Pf6N2E2_4834 at 5587.466 kb on + strand, within Pf6N2E2_4834 at 5587.466 kb on + strand, within Pf6N2E2_4834 at 5587.466 kb on + strand, within Pf6N2E2_4834 at 5587.466 kb on + strand, within Pf6N2E2_4834 at 5587.466 kb on + strand, within Pf6N2E2_4834 at 5587.466 kb on + strand, within Pf6N2E2_4834 at 5587.466 kb on + strand, within Pf6N2E2_4834 at 5587.467 kb on - strand, within Pf6N2E2_4834 at 5587.467 kb on - strand, within Pf6N2E2_4834 at 5587.467 kb on - strand, within Pf6N2E2_4834 at 5587.467 kb on - strand, within Pf6N2E2_4834 at 5587.467 kb on - strand, within Pf6N2E2_4834 at 5587.467 kb on - strand, within Pf6N2E2_4834 at 5587.509 kb on - strand, within Pf6N2E2_4834 at 5587.584 kb on + strand, within Pf6N2E2_4834 at 5587.584 kb on + strand, within Pf6N2E2_4834 at 5587.585 kb on - strand, within Pf6N2E2_4834 at 5587.585 kb on - strand, within Pf6N2E2_4834 at 5587.585 kb on - strand, within Pf6N2E2_4834 at 5587.697 kb on - strand, within Pf6N2E2_4834 at 5587.805 kb on + strand at 5587.806 kb on - strand at 5587.814 kb on + strand at 5587.835 kb on + strand at 5587.835 kb on + strand at 5587.835 kb on + strand at 5587.836 kb on - strand at 5587.836 kb on - strand at 5588.300 kb on - strand at 5588.300 kb on - strand at 5588.302 kb on + strand at 5588.309 kb on + strand at 5588.309 kb on + strand at 5588.309 kb on + strand at 5588.309 kb on + strand at 5588.309 kb on + strand at 5588.310 kb on - strand at 5588.310 kb on - strand at 5588.400 kb on + strand at 5588.400 kb on + strand at 5588.400 kb on + strand at 5588.400 kb on + strand at 5588.415 kb on + strand at 5588.434 kb on + strand at 5588.536 kb on + strand, within Pf6N2E2_4836 at 5588.536 kb on + strand, within Pf6N2E2_4836 at 5588.557 kb on + strand, within Pf6N2E2_4836 at 5588.558 kb on - strand, within Pf6N2E2_4836 at 5588.558 kb on - strand, within Pf6N2E2_4836 at 5588.740 kb on + strand, within Pf6N2E2_4836 at 5588.741 kb on - strand, within Pf6N2E2_4836 at 5588.741 kb on - strand, within Pf6N2E2_4836 at 5588.931 kb on + strand, within Pf6N2E2_4836 at 5588.932 kb on - strand, within Pf6N2E2_4836 at 5588.932 kb on - strand, within Pf6N2E2_4836 at 5589.011 kb on - strand, within Pf6N2E2_4836 at 5589.139 kb on + strand, within Pf6N2E2_4836 at 5589.140 kb on - strand, within Pf6N2E2_4836 at 5589.218 kb on + strand, within Pf6N2E2_4836 at 5589.219 kb on - strand, within Pf6N2E2_4836
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 5,586,882 + -0.6 5,586,883 - -0.6 5,586,963 - Pf6N2E2_4834 0.14 +0.2 5,586,963 - Pf6N2E2_4834 0.14 -0.5 5,587,104 - Pf6N2E2_4834 0.28 -0.1 5,587,114 + Pf6N2E2_4834 0.29 -0.9 5,587,114 + Pf6N2E2_4834 0.29 -0.5 5,587,115 - Pf6N2E2_4834 0.29 -0.5 5,587,115 - Pf6N2E2_4834 0.29 -0.3 5,587,115 - Pf6N2E2_4834 0.29 +0.5 5,587,115 - Pf6N2E2_4834 0.29 -3.0 5,587,157 - Pf6N2E2_4834 0.34 -1.1 5,587,157 - Pf6N2E2_4834 0.34 -0.5 5,587,157 - Pf6N2E2_4834 0.34 -2.1 5,587,157 - Pf6N2E2_4834 0.34 +0.5 5,587,157 - Pf6N2E2_4834 0.34 -0.5 5,587,158 + Pf6N2E2_4834 0.34 -0.5 5,587,158 + Pf6N2E2_4834 0.34 +0.5 5,587,158 + Pf6N2E2_4834 0.34 -0.5 5,587,159 - Pf6N2E2_4834 0.34 +0.5 5,587,210 - Pf6N2E2_4834 0.39 -0.5 5,587,210 - Pf6N2E2_4834 0.39 -0.5 5,587,210 - Pf6N2E2_4834 0.39 +0.5 5,587,280 + Pf6N2E2_4834 0.46 -1.1 5,587,280 + Pf6N2E2_4834 0.46 -1.1 5,587,281 - Pf6N2E2_4834 0.47 -0.5 5,587,281 - Pf6N2E2_4834 0.47 -0.5 5,587,281 - Pf6N2E2_4834 0.47 -1.1 5,587,281 - Pf6N2E2_4834 0.47 +0.9 5,587,281 - Pf6N2E2_4834 0.47 -0.5 5,587,289 - Pf6N2E2_4834 0.47 +0.5 5,587,449 + Pf6N2E2_4834 0.64 +0.5 5,587,449 + Pf6N2E2_4834 0.64 -0.5 5,587,450 - Pf6N2E2_4834 0.64 -0.5 5,587,466 + Pf6N2E2_4834 0.66 -0.4 5,587,466 + Pf6N2E2_4834 0.66 -1.1 5,587,466 + Pf6N2E2_4834 0.66 -0.6 5,587,466 + Pf6N2E2_4834 0.66 -0.7 5,587,466 + Pf6N2E2_4834 0.66 -1.1 5,587,466 + Pf6N2E2_4834 0.66 -0.5 5,587,466 + Pf6N2E2_4834 0.66 -0.2 5,587,466 + Pf6N2E2_4834 0.66 -0.5 5,587,466 + Pf6N2E2_4834 0.66 -1.5 5,587,467 - Pf6N2E2_4834 0.66 -0.3 5,587,467 - Pf6N2E2_4834 0.66 -0.5 5,587,467 - Pf6N2E2_4834 0.66 -0.5 5,587,467 - Pf6N2E2_4834 0.66 +0.5 5,587,467 - Pf6N2E2_4834 0.66 -0.6 5,587,467 - Pf6N2E2_4834 0.66 -1.9 5,587,509 - Pf6N2E2_4834 0.70 -0.8 5,587,584 + Pf6N2E2_4834 0.78 -1.1 5,587,584 + Pf6N2E2_4834 0.78 -0.5 5,587,585 - Pf6N2E2_4834 0.78 +0.1 5,587,585 - Pf6N2E2_4834 0.78 -0.9 5,587,585 - Pf6N2E2_4834 0.78 -0.5 5,587,697 - Pf6N2E2_4834 0.89 -0.7 5,587,805 + -0.7 5,587,806 - -0.7 5,587,814 + -0.2 5,587,835 + -0.5 5,587,835 + +0.5 5,587,835 + -0.5 5,587,836 - -0.5 5,587,836 - +0.5 5,588,300 - -0.5 5,588,300 - +0.5 5,588,302 + -0.5 5,588,309 + +0.5 5,588,309 + -1.1 5,588,309 + +0.5 5,588,309 + -0.6 5,588,309 + -0.4 5,588,310 - +0.5 5,588,310 - -0.5 5,588,400 + -0.5 5,588,400 + -0.5 5,588,400 + -0.5 5,588,400 + -3.3 5,588,415 + -1.1 5,588,434 + -0.5 5,588,536 + Pf6N2E2_4836 0.13 -0.5 5,588,536 + Pf6N2E2_4836 0.13 +0.5 5,588,557 + Pf6N2E2_4836 0.15 -1.1 5,588,558 - Pf6N2E2_4836 0.15 +0.5 5,588,558 - Pf6N2E2_4836 0.15 +0.5 5,588,740 + Pf6N2E2_4836 0.33 -3.0 5,588,741 - Pf6N2E2_4836 0.33 -3.0 5,588,741 - Pf6N2E2_4836 0.33 +0.5 5,588,931 + Pf6N2E2_4836 0.51 -1.5 5,588,932 - Pf6N2E2_4836 0.51 +0.5 5,588,932 - Pf6N2E2_4836 0.51 +0.5 5,589,011 - Pf6N2E2_4836 0.59 -2.1 5,589,139 + Pf6N2E2_4836 0.71 +0.5 5,589,140 - Pf6N2E2_4836 0.71 -0.5 5,589,218 + Pf6N2E2_4836 0.79 -1.1 5,589,219 - Pf6N2E2_4836 0.79 -0.5
Or see this region's nucleotide sequence