Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_481 and Pf6N2E2_482 are separated by 192 nucleotides Pf6N2E2_482 and Pf6N2E2_483 are separated by 151 nucleotides
Pf6N2E2_481 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) <small>(from data)</small>, at 547,221 to 548,456
_481
Pf6N2E2_482 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 548,649 to 549,137
_482
Pf6N2E2_483 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 549,289 to 550,152
_483
Position (kb) 548
549
550 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.957 kb on - strand, within Pf6N2E2_481 at 548.137 kb on + strand, within Pf6N2E2_481 at 548.138 kb on - strand, within Pf6N2E2_481 at 548.185 kb on + strand, within Pf6N2E2_481 at 548.185 kb on + strand at 548.186 kb on - strand, within Pf6N2E2_481 at 548.345 kb on + strand at 548.377 kb on + strand at 548.378 kb on - strand at 548.464 kb on + strand at 548.465 kb on - strand at 548.465 kb on - strand at 548.465 kb on - strand at 548.489 kb on + strand at 548.502 kb on - strand at 548.537 kb on + strand at 548.538 kb on - strand at 548.548 kb on + strand at 548.549 kb on - strand at 548.549 kb on - strand at 548.829 kb on - strand, within Pf6N2E2_482 at 548.943 kb on + strand, within Pf6N2E2_482 at 548.943 kb on + strand, within Pf6N2E2_482 at 549.290 kb on + strand at 549.291 kb on - strand at 549.291 kb on - strand at 549.291 kb on - strand at 549.317 kb on + strand at 549.317 kb on + strand at 549.318 kb on - strand at 549.533 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.549 kb on - strand, within Pf6N2E2_483 at 549.549 kb on - strand, within Pf6N2E2_483 at 549.549 kb on - strand, within Pf6N2E2_483 at 549.596 kb on + strand, within Pf6N2E2_483 at 549.597 kb on - strand, within Pf6N2E2_483 at 549.597 kb on - strand, within Pf6N2E2_483 at 549.698 kb on + strand, within Pf6N2E2_483 at 549.911 kb on + strand, within Pf6N2E2_483 at 549.912 kb on - strand at 549.912 kb on - strand, within Pf6N2E2_483 at 550.100 kb on + strand at 550.100 kb on + strand at 550.100 kb on + strand at 550.100 kb on + strand at 550.100 kb on + strand at 550.101 kb on - strand at 550.101 kb on - strand at 550.101 kb on - strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.107 kb on - strand at 550.107 kb on - strand at 550.107 kb on - strand at 550.107 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 547,945 + Pf6N2E2_481 0.59 +0.3 547,945 + Pf6N2E2_481 0.59 -1.3 547,945 + Pf6N2E2_481 0.59 +0.3 547,945 + Pf6N2E2_481 0.59 -0.7 547,946 - Pf6N2E2_481 0.59 -0.7 547,946 - Pf6N2E2_481 0.59 -2.5 547,946 - Pf6N2E2_481 0.59 +0.3 547,946 - Pf6N2E2_481 0.59 -2.9 547,957 - Pf6N2E2_481 0.60 +0.3 548,137 + Pf6N2E2_481 0.74 +0.3 548,138 - Pf6N2E2_481 0.74 -3.0 548,185 + Pf6N2E2_481 0.78 +0.3 548,185 + +0.3 548,186 - Pf6N2E2_481 0.78 +0.3 548,345 + -1.7 548,377 + -1.1 548,378 - -1.3 548,464 + +0.3 548,465 - +0.3 548,465 - -0.7 548,465 - -0.7 548,489 + -3.0 548,502 - -2.3 548,537 + +0.3 548,538 - -2.0 548,548 + -1.3 548,549 - +0.3 548,549 - +0.3 548,829 - Pf6N2E2_482 0.37 -2.0 548,943 + Pf6N2E2_482 0.60 +0.3 548,943 + Pf6N2E2_482 0.60 -1.7 549,290 + +0.3 549,291 - +0.3 549,291 - +0.3 549,291 - +0.3 549,317 + -1.3 549,317 + -0.7 549,318 - +0.3 549,533 + Pf6N2E2_483 0.28 +0.9 549,548 + Pf6N2E2_483 0.30 +0.3 549,548 + Pf6N2E2_483 0.30 -0.7 549,548 + Pf6N2E2_483 0.30 +0.9 549,548 + Pf6N2E2_483 0.30 +0.3 549,549 - Pf6N2E2_483 0.30 +0.3 549,549 - Pf6N2E2_483 0.30 -0.5 549,549 - Pf6N2E2_483 0.30 +0.1 549,596 + Pf6N2E2_483 0.36 -0.6 549,597 - Pf6N2E2_483 0.36 -0.8 549,597 - Pf6N2E2_483 0.36 -0.7 549,698 + Pf6N2E2_483 0.47 -0.7 549,911 + Pf6N2E2_483 0.72 +0.0 549,912 - +0.3 549,912 - Pf6N2E2_483 0.72 +0.3 550,100 + -0.7 550,100 + +0.3 550,100 + -1.5 550,100 + -0.7 550,100 + +0.3 550,101 - +0.3 550,101 - -0.7 550,101 - +0.3 550,106 + +0.3 550,106 + +0.3 550,106 + +0.3 550,106 + -0.7 550,106 + -0.9 550,107 - +0.3 550,107 - -1.0 550,107 - +0.3 550,107 - -1.3
Or see this region's nucleotide sequence