Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_481

Experiment: Ying_AminoAcid20 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_480 and Pf6N2E2_481 are separated by 1 nucleotidesPf6N2E2_481 and Pf6N2E2_482 are separated by 192 nucleotidesPf6N2E2_482 and Pf6N2E2_483 are separated by 151 nucleotides Pf6N2E2_480 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data), at 546,161 to 547,219 _480 Pf6N2E2_481 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data), at 547,221 to 548,456 _481 Pf6N2E2_482 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 548,649 to 549,137 _482 Pf6N2E2_483 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 549,289 to 550,152 _483 Position (kb) 547 548 549Strain fitness (log2 ratio) -3 -2 -1 0 1at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.499 kb on - strand, within Pf6N2E2_480at 546.499 kb on - strand, within Pf6N2E2_480at 546.499 kb on - strandat 546.561 kb on + strand, within Pf6N2E2_480at 546.561 kb on + strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.565 kb on - strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.646 kb on - strand, within Pf6N2E2_480at 546.646 kb on - strand, within Pf6N2E2_480at 546.721 kb on - strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.805 kb on - strand, within Pf6N2E2_480at 546.805 kb on - strand, within Pf6N2E2_480at 546.913 kb on - strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 547.032 kb on + strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.077 kb on + strand, within Pf6N2E2_480at 547.078 kb on - strandat 547.198 kb on + strandat 547.199 kb on - strandat 547.199 kb on - strandat 547.274 kb on - strandat 547.274 kb on - strandat 547.427 kb on + strand, within Pf6N2E2_481at 547.427 kb on + strand, within Pf6N2E2_481at 547.427 kb on + strand, within Pf6N2E2_481at 547.427 kb on + strand, within Pf6N2E2_481at 547.428 kb on - strand, within Pf6N2E2_481at 547.428 kb on - strand, within Pf6N2E2_481at 547.945 kb on + strand, within Pf6N2E2_481at 547.945 kb on + strand, within Pf6N2E2_481at 547.945 kb on + strand, within Pf6N2E2_481at 547.945 kb on + strand, within Pf6N2E2_481at 547.946 kb on - strand, within Pf6N2E2_481at 547.946 kb on - strand, within Pf6N2E2_481at 547.946 kb on - strand, within Pf6N2E2_481at 547.946 kb on - strand, within Pf6N2E2_481at 547.957 kb on - strand, within Pf6N2E2_481at 548.137 kb on + strand, within Pf6N2E2_481at 548.138 kb on - strand, within Pf6N2E2_481at 548.185 kb on + strand, within Pf6N2E2_481at 548.185 kb on + strandat 548.186 kb on - strand, within Pf6N2E2_481at 548.345 kb on + strandat 548.377 kb on + strandat 548.378 kb on - strandat 548.464 kb on + strandat 548.465 kb on - strandat 548.465 kb on - strandat 548.465 kb on - strandat 548.489 kb on + strandat 548.502 kb on - strandat 548.537 kb on + strandat 548.538 kb on - strandat 548.548 kb on + strandat 548.549 kb on - strandat 548.549 kb on - strandat 548.829 kb on - strand, within Pf6N2E2_482at 548.943 kb on + strand, within Pf6N2E2_482at 548.943 kb on + strand, within Pf6N2E2_482at 549.290 kb on + strandat 549.291 kb on - strandat 549.291 kb on - strandat 549.291 kb on - strandat 549.317 kb on + strandat 549.317 kb on + strandat 549.318 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3
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546,483 + Pf6N2E2_480 0.30 +0.3
546,483 + Pf6N2E2_480 0.30 +0.3
546,483 + Pf6N2E2_480 0.30 +0.3
546,483 + Pf6N2E2_480 0.30 -0.7
546,483 + Pf6N2E2_480 0.30 -2.2
546,484 - Pf6N2E2_480 0.31 -0.7
546,484 - Pf6N2E2_480 0.31 +0.3
546,484 - Pf6N2E2_480 0.31 -0.9
546,484 - Pf6N2E2_480 0.31 -3.5
546,484 - Pf6N2E2_480 0.31 +0.3
546,484 - Pf6N2E2_480 0.31 +0.3
546,484 - Pf6N2E2_480 0.31 +0.3
546,484 - Pf6N2E2_480 0.31 -0.2
546,484 - Pf6N2E2_480 0.31 +0.3
546,484 - Pf6N2E2_480 0.31 +0.3
546,484 - Pf6N2E2_480 0.31 -2.7
546,498 + Pf6N2E2_480 0.32 +0.3
546,498 + Pf6N2E2_480 0.32 +0.3
546,498 + Pf6N2E2_480 0.32 -1.7
546,499 - Pf6N2E2_480 0.32 -0.7
546,499 - Pf6N2E2_480 0.32 +0.3
546,499 - -0.7
546,561 + Pf6N2E2_480 0.38 +0.3
546,561 + Pf6N2E2_480 0.38 +0.3
546,562 - Pf6N2E2_480 0.38 -0.7
546,562 - Pf6N2E2_480 0.38 -2.3
546,562 - Pf6N2E2_480 0.38 -1.3
546,565 - Pf6N2E2_480 0.38 -2.3
546,645 + Pf6N2E2_480 0.46 +0.3
546,645 + Pf6N2E2_480 0.46 -0.7
546,645 + Pf6N2E2_480 0.46 +0.3
546,646 - Pf6N2E2_480 0.46 +0.3
546,646 - Pf6N2E2_480 0.46 +0.3
546,721 - Pf6N2E2_480 0.53 +0.3
546,804 + Pf6N2E2_480 0.61 +0.3
546,804 + Pf6N2E2_480 0.61 +0.3
546,804 + Pf6N2E2_480 0.61 +0.3
546,804 + Pf6N2E2_480 0.61 -0.7
546,804 + Pf6N2E2_480 0.61 +0.3
546,805 - Pf6N2E2_480 0.61 +0.3
546,805 - Pf6N2E2_480 0.61 -0.7
546,913 - Pf6N2E2_480 0.71 +0.3
546,918 + Pf6N2E2_480 0.71 +0.3
546,918 + Pf6N2E2_480 0.71 +0.3
546,918 + Pf6N2E2_480 0.71 +0.3
546,918 + Pf6N2E2_480 0.71 +0.3
546,919 - Pf6N2E2_480 0.72 -0.7
546,919 - Pf6N2E2_480 0.72 +0.3
546,919 - Pf6N2E2_480 0.72 -0.7
546,919 - Pf6N2E2_480 0.72 -1.7
547,032 + Pf6N2E2_480 0.82 +0.3
547,033 - Pf6N2E2_480 0.82 +0.3
547,033 - Pf6N2E2_480 0.82 +0.3
547,033 - Pf6N2E2_480 0.82 -2.0
547,077 + Pf6N2E2_480 0.86 -0.7
547,078 - -0.7
547,198 + +0.3
547,199 - -0.7
547,199 - -3.0
547,274 - -0.7
547,274 - +0.3
547,427 + Pf6N2E2_481 0.17 +0.3
547,427 + Pf6N2E2_481 0.17 -0.7
547,427 + Pf6N2E2_481 0.17 +0.3
547,427 + Pf6N2E2_481 0.17 -1.3
547,428 - Pf6N2E2_481 0.17 -0.7
547,428 - Pf6N2E2_481 0.17 +0.3
547,945 + Pf6N2E2_481 0.59 +0.3
547,945 + Pf6N2E2_481 0.59 -1.3
547,945 + Pf6N2E2_481 0.59 +0.3
547,945 + Pf6N2E2_481 0.59 -0.7
547,946 - Pf6N2E2_481 0.59 -0.7
547,946 - Pf6N2E2_481 0.59 -2.5
547,946 - Pf6N2E2_481 0.59 +0.3
547,946 - Pf6N2E2_481 0.59 -2.9
547,957 - Pf6N2E2_481 0.60 +0.3
548,137 + Pf6N2E2_481 0.74 +0.3
548,138 - Pf6N2E2_481 0.74 -3.0
548,185 + Pf6N2E2_481 0.78 +0.3
548,185 + +0.3
548,186 - Pf6N2E2_481 0.78 +0.3
548,345 + -1.7
548,377 + -1.1
548,378 - -1.3
548,464 + +0.3
548,465 - +0.3
548,465 - -0.7
548,465 - -0.7
548,489 + -3.0
548,502 - -2.3
548,537 + +0.3
548,538 - -2.0
548,548 + -1.3
548,549 - +0.3
548,549 - +0.3
548,829 - Pf6N2E2_482 0.37 -2.0
548,943 + Pf6N2E2_482 0.60 +0.3
548,943 + Pf6N2E2_482 0.60 -1.7
549,290 + +0.3
549,291 - +0.3
549,291 - +0.3
549,291 - +0.3
549,317 + -1.3
549,317 + -0.7
549,318 - +0.3

Or see this region's nucleotide sequence