Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4761 and Pf6N2E2_4762 overlap by 8 nucleotides Pf6N2E2_4762 and Pf6N2E2_4763 overlap by 8 nucleotides Pf6N2E2_4763 and Pf6N2E2_4764 overlap by 4 nucleotides Pf6N2E2_4764 and Pf6N2E2_4765 are separated by 118 nucleotides
Pf6N2E2_4761 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 5,499,374 to 5,500,552
_4761
Pf6N2E2_4762 - Biotin synthesis protein BioH, at 5,500,545 to 5,501,276
_4762
Pf6N2E2_4763 - Biotin synthesis protein BioC, at 5,501,269 to 5,502,081
_4763
Pf6N2E2_4764 - Dethiobiotin synthetase (EC 6.3.3.3), at 5,502,078 to 5,502,758
_4764
Pf6N2E2_4765 - FIG00954771: hypothetical protein, at 5,502,877 to 5,503,173
_4765
Position (kb)
5501
5502
5503 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5500.319 kb on - strand, within Pf6N2E2_4761 at 5500.319 kb on - strand, within Pf6N2E2_4761 at 5500.319 kb on - strand, within Pf6N2E2_4761 at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.492 kb on - strand at 5500.492 kb on - strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.562 kb on - strand at 5500.562 kb on - strand at 5500.987 kb on + strand, within Pf6N2E2_4762 at 5500.987 kb on + strand, within Pf6N2E2_4762 at 5500.988 kb on - strand, within Pf6N2E2_4762 at 5501.057 kb on + strand, within Pf6N2E2_4762 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.464 kb on + strand, within Pf6N2E2_4763 at 5501.465 kb on - strand, within Pf6N2E2_4763 at 5501.465 kb on - strand, within Pf6N2E2_4763 at 5501.735 kb on + strand, within Pf6N2E2_4763 at 5501.735 kb on + strand, within Pf6N2E2_4763 at 5501.736 kb on - strand, within Pf6N2E2_4763 at 5501.818 kb on + strand, within Pf6N2E2_4763 at 5501.818 kb on + strand, within Pf6N2E2_4763 at 5501.818 kb on + strand, within Pf6N2E2_4763 at 5501.818 kb on + strand, within Pf6N2E2_4763 at 5501.818 kb on + strand, within Pf6N2E2_4763 at 5501.819 kb on - strand, within Pf6N2E2_4763 at 5501.819 kb on - strand, within Pf6N2E2_4763 at 5501.819 kb on - strand, within Pf6N2E2_4763 at 5501.819 kb on - strand, within Pf6N2E2_4763 at 5501.819 kb on - strand, within Pf6N2E2_4763 at 5501.888 kb on - strand, within Pf6N2E2_4763 at 5502.004 kb on + strand at 5502.004 kb on + strand at 5502.004 kb on + strand at 5502.004 kb on + strand at 5502.005 kb on - strand at 5502.005 kb on - strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.044 kb on - strand at 5502.044 kb on - strand at 5502.078 kb on - strand at 5502.090 kb on + strand at 5502.091 kb on - strand at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.283 kb on - strand, within Pf6N2E2_4764 at 5502.283 kb on - strand, within Pf6N2E2_4764 at 5502.283 kb on - strand, within Pf6N2E2_4764 at 5502.283 kb on - strand, within Pf6N2E2_4764 at 5502.283 kb on - strand, within Pf6N2E2_4764 at 5502.894 kb on + strand at 5502.904 kb on + strand at 5502.904 kb on + strand at 5502.904 kb on + strand at 5502.904 kb on + strand at 5502.905 kb on - strand at 5502.905 kb on - strand at 5502.905 kb on - strand at 5502.905 kb on - strand at 5502.905 kb on - strand at 5502.975 kb on + strand, within Pf6N2E2_4765 at 5502.976 kb on - strand, within Pf6N2E2_4765
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 5,500,319 - Pf6N2E2_4761 0.80 +0.5 5,500,319 - Pf6N2E2_4761 0.80 -2.8 5,500,319 - Pf6N2E2_4761 0.80 -1.1 5,500,491 + -0.5 5,500,491 + -0.5 5,500,491 + +0.5 5,500,491 + +0.9 5,500,491 + -0.5 5,500,491 + -1.1 5,500,491 + -0.5 5,500,492 - -0.5 5,500,492 - -0.5 5,500,561 + -0.5 5,500,561 + +1.1 5,500,561 + -0.4 5,500,561 + -1.5 5,500,561 + -1.5 5,500,561 + +2.1 5,500,562 - +0.1 5,500,562 - -1.1 5,500,987 + Pf6N2E2_4762 0.60 -1.5 5,500,987 + Pf6N2E2_4762 0.60 +0.1 5,500,988 - Pf6N2E2_4762 0.61 -0.5 5,501,057 + Pf6N2E2_4762 0.70 -1.1 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 -0.5 5,501,356 + Pf6N2E2_4763 0.11 -0.5 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 -2.3 5,501,356 + Pf6N2E2_4763 0.11 -0.5 5,501,356 + Pf6N2E2_4763 0.11 -1.5 5,501,356 + Pf6N2E2_4763 0.11 -1.8 5,501,356 + Pf6N2E2_4763 0.11 -1.1 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 -1.1 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,357 - Pf6N2E2_4763 0.11 -0.5 5,501,357 - Pf6N2E2_4763 0.11 -1.8 5,501,357 - Pf6N2E2_4763 0.11 +0.5 5,501,357 - Pf6N2E2_4763 0.11 +0.5 5,501,464 + Pf6N2E2_4763 0.24 -0.5 5,501,465 - Pf6N2E2_4763 0.24 +0.5 5,501,465 - Pf6N2E2_4763 0.24 -0.5 5,501,735 + Pf6N2E2_4763 0.57 -1.5 5,501,735 + Pf6N2E2_4763 0.57 +0.5 5,501,736 - Pf6N2E2_4763 0.57 +0.5 5,501,818 + Pf6N2E2_4763 0.68 -0.5 5,501,818 + Pf6N2E2_4763 0.68 +0.5 5,501,818 + Pf6N2E2_4763 0.68 -0.5 5,501,818 + Pf6N2E2_4763 0.68 +0.5 5,501,818 + Pf6N2E2_4763 0.68 +0.5 5,501,819 - Pf6N2E2_4763 0.68 -0.5 5,501,819 - Pf6N2E2_4763 0.68 -0.5 5,501,819 - Pf6N2E2_4763 0.68 -0.5 5,501,819 - Pf6N2E2_4763 0.68 -1.1 5,501,819 - Pf6N2E2_4763 0.68 -0.5 5,501,888 - Pf6N2E2_4763 0.76 +0.5 5,502,004 + +0.1 5,502,004 + +0.5 5,502,004 + +0.5 5,502,004 + -1.8 5,502,005 - +0.5 5,502,005 - +0.5 5,502,043 + +0.5 5,502,043 + -1.1 5,502,043 + +0.5 5,502,043 + +0.5 5,502,043 + +0.5 5,502,044 - -0.5 5,502,044 - +0.5 5,502,078 - +0.5 5,502,090 + +0.5 5,502,091 - +0.5 5,502,282 + Pf6N2E2_4764 0.30 -0.5 5,502,282 + Pf6N2E2_4764 0.30 -1.1 5,502,282 + Pf6N2E2_4764 0.30 +0.5 5,502,282 + Pf6N2E2_4764 0.30 +0.5 5,502,282 + Pf6N2E2_4764 0.30 +0.5 5,502,282 + Pf6N2E2_4764 0.30 -1.1 5,502,282 + Pf6N2E2_4764 0.30 +0.5 5,502,282 + Pf6N2E2_4764 0.30 +0.5 5,502,283 - Pf6N2E2_4764 0.30 -0.5 5,502,283 - Pf6N2E2_4764 0.30 +0.5 5,502,283 - Pf6N2E2_4764 0.30 +0.5 5,502,283 - Pf6N2E2_4764 0.30 -0.5 5,502,283 - Pf6N2E2_4764 0.30 -0.5 5,502,894 + -0.5 5,502,904 + -0.5 5,502,904 + -0.5 5,502,904 + -0.5 5,502,904 + +0.5 5,502,905 - -0.5 5,502,905 - -0.5 5,502,905 - -0.5 5,502,905 - -0.8 5,502,905 - +0.5 5,502,975 + Pf6N2E2_4765 0.33 -0.5 5,502,976 - Pf6N2E2_4765 0.33 +2.1
Or see this region's nucleotide sequence