Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_473

Experiment: Ying_AminoAcid20 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_471 and Pf6N2E2_472 are separated by 288 nucleotidesPf6N2E2_472 and Pf6N2E2_473 are separated by 13 nucleotidesPf6N2E2_473 and Pf6N2E2_474 are separated by 82 nucleotidesPf6N2E2_474 and Pf6N2E2_475 are separated by 76 nucleotides Pf6N2E2_471 - putative metalloprotease, at 536,431 to 537,492 _471 Pf6N2E2_472 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 537,781 to 538,122 _472 Pf6N2E2_473 - putative cytochrome p450 oxidoreductase, at 538,136 to 539,272 _473 Pf6N2E2_474 - Transcriptional regulator, ArsR family, at 539,355 to 540,110 _474 Pf6N2E2_475 - Transcriptional regulator, AraC family, at 540,187 to 540,981 _475 Position (kb) 538 539 540Strain fitness (log2 ratio) -3 -2 -1 0 1at 537.170 kb on + strand, within Pf6N2E2_471at 537.170 kb on + strand, within Pf6N2E2_471at 537.170 kb on + strand, within Pf6N2E2_471at 537.170 kb on + strand, within Pf6N2E2_471at 537.171 kb on - strand, within Pf6N2E2_471at 537.171 kb on - strand, within Pf6N2E2_471at 537.452 kb on + strandat 537.452 kb on + strandat 537.452 kb on + strandat 537.453 kb on - strandat 537.453 kb on - strandat 537.453 kb on - strandat 537.453 kb on - strandat 537.505 kb on - strandat 537.833 kb on + strand, within Pf6N2E2_472at 537.833 kb on + strand, within Pf6N2E2_472at 537.833 kb on + strand, within Pf6N2E2_472at 537.904 kb on + strand, within Pf6N2E2_472at 537.904 kb on + strand, within Pf6N2E2_472at 537.905 kb on - strand, within Pf6N2E2_472at 537.905 kb on - strand, within Pf6N2E2_472at 538.031 kb on - strand, within Pf6N2E2_472at 538.212 kb on - strandat 538.617 kb on + strand, within Pf6N2E2_473at 538.617 kb on + strand, within Pf6N2E2_473at 538.617 kb on + strand, within Pf6N2E2_473at 538.617 kb on + strand, within Pf6N2E2_473at 538.618 kb on - strand, within Pf6N2E2_473at 538.655 kb on - strand, within Pf6N2E2_473at 538.655 kb on - strand, within Pf6N2E2_473at 538.736 kb on + strand, within Pf6N2E2_473at 539.227 kb on - strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.310 kb on - strandat 539.310 kb on - strandat 539.312 kb on + strandat 539.577 kb on + strand, within Pf6N2E2_474at 539.577 kb on + strand, within Pf6N2E2_474at 539.578 kb on - strand, within Pf6N2E2_474at 539.578 kb on - strand, within Pf6N2E2_474at 539.578 kb on - strand, within Pf6N2E2_474at 539.697 kb on + strand, within Pf6N2E2_474at 539.705 kb on + strand, within Pf6N2E2_474at 539.706 kb on - strand, within Pf6N2E2_474at 540.198 kb on + strandat 540.198 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3
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537,170 + Pf6N2E2_471 0.70 -1.7
537,170 + Pf6N2E2_471 0.70 +0.3
537,170 + Pf6N2E2_471 0.70 -0.7
537,170 + Pf6N2E2_471 0.70 +0.3
537,171 - Pf6N2E2_471 0.70 -2.3
537,171 - Pf6N2E2_471 0.70 +0.3
537,452 + +0.3
537,452 + -1.3
537,452 + +0.3
537,453 - +0.3
537,453 - +0.3
537,453 - +0.3
537,453 - +0.3
537,505 - +0.3
537,833 + Pf6N2E2_472 0.15 +0.3
537,833 + Pf6N2E2_472 0.15 -0.7
537,833 + Pf6N2E2_472 0.15 -1.7
537,904 + Pf6N2E2_472 0.36 -0.7
537,904 + Pf6N2E2_472 0.36 -0.7
537,905 - Pf6N2E2_472 0.36 +0.3
537,905 - Pf6N2E2_472 0.36 +0.3
538,031 - Pf6N2E2_472 0.73 -0.7
538,212 - -1.3
538,617 + Pf6N2E2_473 0.42 +0.3
538,617 + Pf6N2E2_473 0.42 -1.3
538,617 + Pf6N2E2_473 0.42 +0.3
538,617 + Pf6N2E2_473 0.42 -2.9
538,618 - Pf6N2E2_473 0.42 -1.3
538,655 - Pf6N2E2_473 0.46 -0.7
538,655 - Pf6N2E2_473 0.46 +0.3
538,736 + Pf6N2E2_473 0.53 +0.3
539,227 - -0.5
539,309 + -0.7
539,309 + +0.3
539,309 + -2.5
539,309 + +0.3
539,309 + -0.9
539,309 + -1.1
539,309 + +0.3
539,310 - +0.3
539,310 - +0.3
539,312 + +0.3
539,577 + Pf6N2E2_474 0.29 +0.4
539,577 + Pf6N2E2_474 0.29 +0.0
539,578 - Pf6N2E2_474 0.29 -0.7
539,578 - Pf6N2E2_474 0.29 +0.3
539,578 - Pf6N2E2_474 0.29 -0.3
539,697 + Pf6N2E2_474 0.45 -1.4
539,705 + Pf6N2E2_474 0.46 +0.3
539,706 - Pf6N2E2_474 0.46 +0.3
540,198 + +0.3
540,198 + +0.3

Or see this region's nucleotide sequence