Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4677 and Pf6N2E2_4678 overlap by 9 nucleotides Pf6N2E2_4678 and Pf6N2E2_4679 overlap by 19 nucleotides
Pf6N2E2_4677 - Choline dehydrogenase (EC 1.1.99.1), at 5,410,297 to 5,412,000
_4677
Pf6N2E2_4678 - hypothetical protein, at 5,411,992 to 5,412,231
_4678
Pf6N2E2_4679 - Betaine aldehyde dehydrogenase (EC 1.2.1.8), at 5,412,213 to 5,413,685
_4679
Position (kb)
5411
5412
5413 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5411.033 kb on + strand, within Pf6N2E2_4677 at 5411.052 kb on - strand, within Pf6N2E2_4677 at 5411.078 kb on + strand, within Pf6N2E2_4677 at 5411.078 kb on + strand, within Pf6N2E2_4677 at 5411.079 kb on - strand, within Pf6N2E2_4677 at 5411.079 kb on - strand, within Pf6N2E2_4677 at 5411.079 kb on - strand, within Pf6N2E2_4677 at 5411.087 kb on + strand, within Pf6N2E2_4677 at 5411.087 kb on + strand, within Pf6N2E2_4677 at 5411.087 kb on + strand, within Pf6N2E2_4677 at 5411.087 kb on + strand, within Pf6N2E2_4677 at 5411.088 kb on - strand, within Pf6N2E2_4677 at 5411.088 kb on - strand, within Pf6N2E2_4677 at 5411.384 kb on + strand, within Pf6N2E2_4677 at 5411.385 kb on - strand, within Pf6N2E2_4677 at 5411.497 kb on + strand, within Pf6N2E2_4677 at 5411.498 kb on - strand, within Pf6N2E2_4677 at 5411.625 kb on - strand, within Pf6N2E2_4677 at 5411.625 kb on - strand, within Pf6N2E2_4677 at 5411.642 kb on + strand, within Pf6N2E2_4677 at 5411.642 kb on + strand, within Pf6N2E2_4677 at 5411.643 kb on - strand, within Pf6N2E2_4677 at 5411.643 kb on - strand, within Pf6N2E2_4677 at 5411.643 kb on - strand, within Pf6N2E2_4677 at 5411.684 kb on + strand, within Pf6N2E2_4677 at 5411.685 kb on - strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.805 kb on - strand, within Pf6N2E2_4677 at 5411.981 kb on + strand at 5411.981 kb on + strand at 5411.982 kb on - strand at 5411.989 kb on + strand at 5411.989 kb on + strand at 5411.990 kb on - strand at 5412.099 kb on - strand, within Pf6N2E2_4678 at 5412.249 kb on + strand at 5412.319 kb on + strand at 5412.375 kb on - strand, within Pf6N2E2_4679 at 5412.493 kb on + strand, within Pf6N2E2_4679 at 5412.494 kb on - strand, within Pf6N2E2_4679 at 5412.668 kb on - strand, within Pf6N2E2_4679 at 5412.668 kb on - strand, within Pf6N2E2_4679 at 5412.668 kb on - strand, within Pf6N2E2_4679 at 5412.847 kb on + strand, within Pf6N2E2_4679 at 5412.847 kb on + strand, within Pf6N2E2_4679 at 5412.847 kb on + strand, within Pf6N2E2_4679 at 5412.848 kb on - strand, within Pf6N2E2_4679 at 5412.848 kb on - strand, within Pf6N2E2_4679 at 5413.111 kb on + strand, within Pf6N2E2_4679 at 5413.112 kb on - strand, within Pf6N2E2_4679 at 5413.112 kb on - strand, within Pf6N2E2_4679 at 5413.112 kb on - strand, within Pf6N2E2_4679
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 5,411,033 + Pf6N2E2_4677 0.43 +0.5 5,411,052 - Pf6N2E2_4677 0.44 -0.5 5,411,078 + Pf6N2E2_4677 0.46 -0.9 5,411,078 + Pf6N2E2_4677 0.46 -0.5 5,411,079 - Pf6N2E2_4677 0.46 +0.2 5,411,079 - Pf6N2E2_4677 0.46 -0.2 5,411,079 - Pf6N2E2_4677 0.46 -0.5 5,411,087 + Pf6N2E2_4677 0.46 -0.1 5,411,087 + Pf6N2E2_4677 0.46 +0.5 5,411,087 + Pf6N2E2_4677 0.46 -0.5 5,411,087 + Pf6N2E2_4677 0.46 -0.2 5,411,088 - Pf6N2E2_4677 0.46 +1.1 5,411,088 - Pf6N2E2_4677 0.46 -1.1 5,411,384 + Pf6N2E2_4677 0.64 -0.5 5,411,385 - Pf6N2E2_4677 0.64 -0.5 5,411,497 + Pf6N2E2_4677 0.70 +2.5 5,411,498 - Pf6N2E2_4677 0.70 -0.5 5,411,625 - Pf6N2E2_4677 0.78 +0.4 5,411,625 - Pf6N2E2_4677 0.78 -0.7 5,411,642 + Pf6N2E2_4677 0.79 +0.5 5,411,642 + Pf6N2E2_4677 0.79 +0.5 5,411,643 - Pf6N2E2_4677 0.79 -0.8 5,411,643 - Pf6N2E2_4677 0.79 -1.1 5,411,643 - Pf6N2E2_4677 0.79 -0.3 5,411,684 + Pf6N2E2_4677 0.81 -0.8 5,411,685 - Pf6N2E2_4677 0.81 +0.5 5,411,708 + Pf6N2E2_4677 0.83 -0.5 5,411,708 + Pf6N2E2_4677 0.83 +0.3 5,411,708 + Pf6N2E2_4677 0.83 -0.5 5,411,708 + Pf6N2E2_4677 0.83 -0.5 5,411,708 + Pf6N2E2_4677 0.83 -1.5 5,411,708 + Pf6N2E2_4677 0.83 +0.5 5,411,708 + Pf6N2E2_4677 0.83 -0.5 5,411,708 + Pf6N2E2_4677 0.83 +0.5 5,411,709 - Pf6N2E2_4677 0.83 +0.5 5,411,709 - Pf6N2E2_4677 0.83 -0.5 5,411,709 - Pf6N2E2_4677 0.83 -2.3 5,411,709 - Pf6N2E2_4677 0.83 +0.5 5,411,709 - Pf6N2E2_4677 0.83 -0.5 5,411,805 - Pf6N2E2_4677 0.88 -0.4 5,411,981 + -0.5 5,411,981 + -0.5 5,411,982 - +0.5 5,411,989 + +0.5 5,411,989 + +0.5 5,411,990 - -0.5 5,412,099 - Pf6N2E2_4678 0.45 -1.8 5,412,249 + +0.5 5,412,319 + -0.5 5,412,375 - Pf6N2E2_4679 0.11 +0.5 5,412,493 + Pf6N2E2_4679 0.19 -0.5 5,412,494 - Pf6N2E2_4679 0.19 -0.5 5,412,668 - Pf6N2E2_4679 0.31 -1.1 5,412,668 - Pf6N2E2_4679 0.31 +0.5 5,412,668 - Pf6N2E2_4679 0.31 +0.5 5,412,847 + Pf6N2E2_4679 0.43 -0.5 5,412,847 + Pf6N2E2_4679 0.43 -1.1 5,412,847 + Pf6N2E2_4679 0.43 +0.5 5,412,848 - Pf6N2E2_4679 0.43 -0.5 5,412,848 - Pf6N2E2_4679 0.43 +0.5 5,413,111 + Pf6N2E2_4679 0.61 +0.5 5,413,112 - Pf6N2E2_4679 0.61 -1.8 5,413,112 - Pf6N2E2_4679 0.61 -1.1 5,413,112 - Pf6N2E2_4679 0.61 +0.5
Or see this region's nucleotide sequence