Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4531 and Pf6N2E2_4532 are separated by 2 nucleotides Pf6N2E2_4532 and Pf6N2E2_4533 overlap by 14 nucleotides Pf6N2E2_4533 and Pf6N2E2_4534 are separated by 115 nucleotides Pf6N2E2_4534 and Pf6N2E2_4535 overlap by 4 nucleotides
Pf6N2E2_4531 - hypothetical protein, at 5,266,100 to 5,266,993
_4531
Pf6N2E2_4532 - Flagellum-specific ATP synthase FliI, at 5,266,996 to 5,268,351
_4532
Pf6N2E2_4533 - RspO, at 5,268,338 to 5,268,757
_4533
Pf6N2E2_4534 - hypothetical protein, at 5,268,873 to 5,269,319
_4534
Pf6N2E2_4535 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 5,269,316 to 5,270,419
_4535
Position (kb) 5266
5267
5268
5269 Strain fitness (log2 ratio) -1
0
1 at 5266.053 kb on - strand at 5266.053 kb on - strand at 5266.206 kb on - strand, within Pf6N2E2_4531 at 5266.977 kb on - strand at 5266.977 kb on - strand at 5267.513 kb on + strand, within Pf6N2E2_4532 at 5267.514 kb on - strand, within Pf6N2E2_4532 at 5267.885 kb on - strand, within Pf6N2E2_4532 at 5267.950 kb on + strand, within Pf6N2E2_4532 at 5267.951 kb on - strand, within Pf6N2E2_4532 at 5267.951 kb on - strand, within Pf6N2E2_4532 at 5267.952 kb on + strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5268.003 kb on - strand, within Pf6N2E2_4532 at 5268.337 kb on + strand at 5268.337 kb on + strand at 5268.337 kb on + strand at 5268.338 kb on - strand at 5268.728 kb on + strand at 5268.832 kb on - strand at 5268.939 kb on + strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 5,266,053 - -1.0 5,266,053 - -0.5 5,266,206 - Pf6N2E2_4531 0.12 +0.5 5,266,977 - -1.3 5,266,977 - +0.5 5,267,513 + Pf6N2E2_4532 0.38 -0.4 5,267,514 - Pf6N2E2_4532 0.38 +0.3 5,267,885 - Pf6N2E2_4532 0.66 +0.5 5,267,950 + Pf6N2E2_4532 0.70 +0.5 5,267,951 - Pf6N2E2_4532 0.70 -0.5 5,267,951 - Pf6N2E2_4532 0.70 -0.5 5,267,952 + Pf6N2E2_4532 0.71 -0.5 5,267,953 - Pf6N2E2_4532 0.71 -0.3 5,267,953 - Pf6N2E2_4532 0.71 +0.5 5,267,953 - Pf6N2E2_4532 0.71 -0.0 5,267,953 - Pf6N2E2_4532 0.71 -1.0 5,268,003 - Pf6N2E2_4532 0.74 -0.5 5,268,337 + -0.5 5,268,337 + +0.5 5,268,337 + +0.5 5,268,338 - +0.5 5,268,728 + +0.5 5,268,832 - -1.0 5,268,939 + Pf6N2E2_4534 0.15 -0.4 5,269,208 - Pf6N2E2_4534 0.75 -1.0 5,269,208 - Pf6N2E2_4534 0.75 +0.5 5,269,208 - Pf6N2E2_4534 0.75 +0.5
Or see this region's nucleotide sequence