Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4524 and Pf6N2E2_4525 overlap by 4 nucleotides Pf6N2E2_4525 and Pf6N2E2_4526 are separated by 62 nucleotides Pf6N2E2_4526 and Pf6N2E2_4527 overlap by 4 nucleotides Pf6N2E2_4527 and Pf6N2E2_4528 are separated by 226 nucleotides Pf6N2E2_4528 and Pf6N2E2_4529 overlap by 30 nucleotides
Pf6N2E2_4524 - putative cytochrome P450 hydroxylase, at 5,259,871 to 5,261,088
_4524
Pf6N2E2_4525 - hypothetical protein, at 5,261,085 to 5,261,213
_4525
Pf6N2E2_4526 - Phosphinothricin N-acetyltransferase (EC 2.3.1.-), at 5,261,276 to 5,261,872
_4526
Pf6N2E2_4527 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,261,869 to 5,262,420
_4527
Pf6N2E2_4528 - hypothetical protein, at 5,262,647 to 5,262,760
_4528
Pf6N2E2_4529 - type III secretion protein HrpJ, at 5,262,731 to 5,263,858
_4529
Position (kb) 5261
5262
5263 Strain fitness (log2 ratio) -2
-1
0
1 at 5260.915 kb on + strand, within Pf6N2E2_4524 at 5260.915 kb on + strand, within Pf6N2E2_4524 at 5260.915 kb on + strand, within Pf6N2E2_4524 at 5260.973 kb on + strand at 5260.973 kb on + strand at 5260.974 kb on - strand at 5260.974 kb on - strand at 5261.019 kb on - strand at 5261.030 kb on - strand at 5261.359 kb on - strand, within Pf6N2E2_4526 at 5261.439 kb on - strand, within Pf6N2E2_4526 at 5261.499 kb on + strand, within Pf6N2E2_4526 at 5261.500 kb on - strand, within Pf6N2E2_4526 at 5261.570 kb on + strand, within Pf6N2E2_4526 at 5261.571 kb on - strand, within Pf6N2E2_4526 at 5261.574 kb on + strand, within Pf6N2E2_4526 at 5261.574 kb on + strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.674 kb on - strand, within Pf6N2E2_4526 at 5261.674 kb on - strand, within Pf6N2E2_4526 at 5261.674 kb on - strand, within Pf6N2E2_4526 at 5261.674 kb on - strand, within Pf6N2E2_4526 at 5261.674 kb on - strand, within Pf6N2E2_4526 at 5261.758 kb on + strand, within Pf6N2E2_4526 at 5261.758 kb on + strand, within Pf6N2E2_4526 at 5261.759 kb on - strand, within Pf6N2E2_4526 at 5261.786 kb on - strand, within Pf6N2E2_4526 at 5261.786 kb on - strand, within Pf6N2E2_4526 at 5261.888 kb on + strand at 5262.158 kb on + strand, within Pf6N2E2_4527 at 5262.159 kb on - strand, within Pf6N2E2_4527 at 5262.159 kb on - strand, within Pf6N2E2_4527 at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.439 kb on - strand at 5262.514 kb on - strand at 5262.556 kb on + strand at 5262.738 kb on + strand at 5262.738 kb on + strand at 5262.739 kb on - strand at 5262.739 kb on - strand at 5262.739 kb on - strand at 5262.760 kb on + strand at 5262.892 kb on + strand, within Pf6N2E2_4529 at 5262.893 kb on - strand, within Pf6N2E2_4529 at 5262.893 kb on - strand, within Pf6N2E2_4529 at 5263.197 kb on + strand at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.305 kb on - strand, within Pf6N2E2_4529 at 5263.307 kb on + strand, within Pf6N2E2_4529 at 5263.307 kb on + strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 5,260,915 + Pf6N2E2_4524 0.86 -0.0 5,260,915 + Pf6N2E2_4524 0.86 -0.9 5,260,915 + Pf6N2E2_4524 0.86 +0.5 5,260,973 + -1.0 5,260,973 + +0.5 5,260,974 - +0.5 5,260,974 - +0.5 5,261,019 - +0.5 5,261,030 - -0.5 5,261,359 - Pf6N2E2_4526 0.14 +0.5 5,261,439 - Pf6N2E2_4526 0.27 +0.5 5,261,499 + Pf6N2E2_4526 0.37 +0.5 5,261,500 - Pf6N2E2_4526 0.38 -0.5 5,261,570 + Pf6N2E2_4526 0.49 -0.5 5,261,571 - Pf6N2E2_4526 0.49 -0.4 5,261,574 + Pf6N2E2_4526 0.50 +0.5 5,261,574 + Pf6N2E2_4526 0.50 -0.5 5,261,575 - Pf6N2E2_4526 0.50 -0.8 5,261,575 - Pf6N2E2_4526 0.50 +0.5 5,261,575 - Pf6N2E2_4526 0.50 +0.5 5,261,575 - Pf6N2E2_4526 0.50 -1.8 5,261,575 - Pf6N2E2_4526 0.50 -0.5 5,261,575 - Pf6N2E2_4526 0.50 +0.5 5,261,673 + Pf6N2E2_4526 0.66 +0.5 5,261,673 + Pf6N2E2_4526 0.66 +0.5 5,261,673 + Pf6N2E2_4526 0.66 -1.0 5,261,673 + Pf6N2E2_4526 0.66 +0.5 5,261,673 + Pf6N2E2_4526 0.66 -1.5 5,261,673 + Pf6N2E2_4526 0.66 -1.3 5,261,673 + Pf6N2E2_4526 0.66 -0.7 5,261,673 + Pf6N2E2_4526 0.66 -0.5 5,261,674 - Pf6N2E2_4526 0.67 -1.0 5,261,674 - Pf6N2E2_4526 0.67 -1.5 5,261,674 - Pf6N2E2_4526 0.67 -0.5 5,261,674 - Pf6N2E2_4526 0.67 -0.5 5,261,674 - Pf6N2E2_4526 0.67 -0.7 5,261,758 + Pf6N2E2_4526 0.81 +0.5 5,261,758 + Pf6N2E2_4526 0.81 -1.0 5,261,759 - Pf6N2E2_4526 0.81 -0.5 5,261,786 - Pf6N2E2_4526 0.85 -0.5 5,261,786 - Pf6N2E2_4526 0.85 -0.5 5,261,888 + +0.5 5,262,158 + Pf6N2E2_4527 0.52 +0.0 5,262,159 - Pf6N2E2_4527 0.53 +1.5 5,262,159 - Pf6N2E2_4527 0.53 -2.0 5,262,424 + -0.5 5,262,424 + -0.5 5,262,424 + -1.0 5,262,424 + -1.0 5,262,425 - +0.5 5,262,425 - +0.1 5,262,425 - +0.5 5,262,425 - -0.5 5,262,439 - -0.5 5,262,514 - -0.5 5,262,556 + +0.5 5,262,738 + -0.5 5,262,738 + -0.5 5,262,739 - -1.0 5,262,739 - +0.5 5,262,739 - -0.5 5,262,760 + -0.5 5,262,892 + Pf6N2E2_4529 0.14 -0.5 5,262,893 - Pf6N2E2_4529 0.14 -0.5 5,262,893 - Pf6N2E2_4529 0.14 -0.5 5,263,197 + +0.5 5,263,198 - Pf6N2E2_4529 0.41 -1.0 5,263,198 - Pf6N2E2_4529 0.41 -0.9 5,263,198 - Pf6N2E2_4529 0.41 -0.0 5,263,304 + Pf6N2E2_4529 0.51 +0.5 5,263,304 + Pf6N2E2_4529 0.51 -0.5 5,263,304 + Pf6N2E2_4529 0.51 +0.5 5,263,305 - Pf6N2E2_4529 0.51 -0.3 5,263,307 + Pf6N2E2_4529 0.51 -0.6 5,263,307 + Pf6N2E2_4529 0.51 -0.7 5,263,308 - Pf6N2E2_4529 0.51 +0.5 5,263,308 - Pf6N2E2_4529 0.51 +0.5 5,263,308 - Pf6N2E2_4529 0.51 +0.5
Or see this region's nucleotide sequence