Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4506 and Pf6N2E2_4507 are separated by 25 nucleotides Pf6N2E2_4507 and Pf6N2E2_4508 are separated by 185 nucleotides Pf6N2E2_4508 and Pf6N2E2_4509 are separated by 310 nucleotides
Pf6N2E2_4506 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 5,238,056 to 5,238,862
_4506
Pf6N2E2_4507 - Organosulfonate utilization protein SsuF, at 5,238,888 to 5,239,103
_4507
Pf6N2E2_4508 - Transcriptional regulator, TetR family, at 5,239,289 to 5,239,921
_4508
Pf6N2E2_4509 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data) , at 5,240,232 to 5,241,503
_4509
Position (kb)
5239
5240 Strain fitness (log2 ratio)
-2
-1
0
1 at 5238.378 kb on + strand, within Pf6N2E2_4506 at 5238.378 kb on + strand, within Pf6N2E2_4506 at 5238.872 kb on + strand at 5238.872 kb on + strand at 5238.873 kb on - strand at 5238.873 kb on - strand at 5238.893 kb on + strand at 5238.894 kb on - strand at 5238.894 kb on - strand at 5239.089 kb on - strand at 5239.204 kb on + strand at 5239.205 kb on - strand at 5239.311 kb on + strand at 5239.311 kb on + strand at 5239.312 kb on - strand at 5239.323 kb on + strand at 5239.323 kb on + strand at 5239.323 kb on + strand at 5239.324 kb on - strand at 5239.324 kb on - strand at 5239.335 kb on + strand at 5239.335 kb on + strand at 5239.335 kb on + strand at 5239.336 kb on - strand at 5239.336 kb on - strand at 5239.398 kb on - strand, within Pf6N2E2_4508 at 5239.410 kb on + strand, within Pf6N2E2_4508 at 5239.605 kb on + strand, within Pf6N2E2_4508 at 5239.605 kb on + strand, within Pf6N2E2_4508 at 5239.605 kb on + strand, within Pf6N2E2_4508 at 5239.606 kb on - strand, within Pf6N2E2_4508 at 5239.606 kb on - strand, within Pf6N2E2_4508 at 5239.803 kb on + strand, within Pf6N2E2_4508 at 5239.804 kb on - strand, within Pf6N2E2_4508 at 5239.952 kb on + strand at 5239.952 kb on + strand at 5239.953 kb on - strand at 5239.953 kb on - strand at 5239.953 kb on - strand at 5239.953 kb on - strand at 5239.953 kb on - strand at 5239.976 kb on + strand at 5239.976 kb on + strand at 5239.976 kb on + strand at 5239.977 kb on - strand at 5239.980 kb on + strand at 5239.980 kb on + strand at 5239.980 kb on + strand at 5239.980 kb on + strand at 5239.980 kb on + strand at 5239.981 kb on - strand at 5239.981 kb on - strand at 5239.981 kb on - strand at 5239.981 kb on - strand at 5239.981 kb on - strand at 5240.021 kb on - strand at 5240.021 kb on - strand at 5240.021 kb on - strand at 5240.021 kb on - strand at 5240.039 kb on + strand at 5240.040 kb on - strand at 5240.040 kb on - strand at 5240.067 kb on - strand at 5240.099 kb on + strand at 5240.099 kb on + strand at 5240.099 kb on + strand at 5240.099 kb on + strand at 5240.099 kb on + strand at 5240.100 kb on - strand at 5240.100 kb on - strand at 5240.100 kb on - strand at 5240.100 kb on - strand at 5240.127 kb on - strand at 5240.179 kb on + strand at 5240.180 kb on - strand at 5240.221 kb on - strand at 5240.233 kb on + strand at 5240.234 kb on - strand at 5240.234 kb on - strand at 5240.240 kb on + strand at 5240.241 kb on - strand at 5240.241 kb on - strand at 5240.248 kb on + strand at 5240.248 kb on + strand at 5240.248 kb on + strand at 5240.257 kb on + strand at 5240.258 kb on - strand at 5240.258 kb on - strand at 5240.258 kb on - strand at 5240.321 kb on + strand at 5240.321 kb on + strand at 5240.322 kb on - strand at 5240.322 kb on - strand at 5240.322 kb on - strand at 5240.329 kb on + strand at 5240.344 kb on + strand at 5240.344 kb on + strand at 5240.345 kb on - strand at 5240.345 kb on - strand at 5240.345 kb on - strand at 5240.425 kb on + strand, within Pf6N2E2_4509 at 5240.425 kb on + strand, within Pf6N2E2_4509 at 5240.425 kb on + strand, within Pf6N2E2_4509 at 5240.426 kb on - strand, within Pf6N2E2_4509 at 5240.426 kb on - strand, within Pf6N2E2_4509 at 5240.513 kb on - strand, within Pf6N2E2_4509 at 5240.632 kb on + strand, within Pf6N2E2_4509 at 5240.633 kb on - strand, within Pf6N2E2_4509 at 5240.633 kb on - strand, within Pf6N2E2_4509 at 5240.657 kb on - strand, within Pf6N2E2_4509 at 5240.657 kb on - strand, within Pf6N2E2_4509 at 5240.784 kb on - strand, within Pf6N2E2_4509 at 5240.784 kb on - strand, within Pf6N2E2_4509 at 5240.872 kb on + strand, within Pf6N2E2_4509 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5240.911 kb on + strand, within Pf6N2E2_4509
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 5,238,378 + Pf6N2E2_4506 0.40 +0.5 5,238,378 + Pf6N2E2_4506 0.40 -0.5 5,238,872 + -0.5 5,238,872 + -1.0 5,238,873 - -0.4 5,238,873 - -0.5 5,238,893 + +0.5 5,238,894 - -1.5 5,238,894 - -0.7 5,239,089 - -0.5 5,239,204 + -0.2 5,239,205 - +0.5 5,239,311 + -0.5 5,239,311 + +0.5 5,239,312 - +0.1 5,239,323 + -0.8 5,239,323 + -1.0 5,239,323 + -0.6 5,239,324 - +0.5 5,239,324 - +0.5 5,239,335 + +0.5 5,239,335 + -1.6 5,239,335 + -0.5 5,239,336 - +0.5 5,239,336 - -0.8 5,239,398 - Pf6N2E2_4508 0.17 -1.0 5,239,410 + Pf6N2E2_4508 0.19 -0.5 5,239,605 + Pf6N2E2_4508 0.50 +0.5 5,239,605 + Pf6N2E2_4508 0.50 -1.0 5,239,605 + Pf6N2E2_4508 0.50 -0.5 5,239,606 - Pf6N2E2_4508 0.50 -0.5 5,239,606 - Pf6N2E2_4508 0.50 +0.5 5,239,803 + Pf6N2E2_4508 0.81 +0.5 5,239,804 - Pf6N2E2_4508 0.81 -1.0 5,239,952 + -0.3 5,239,952 + -0.5 5,239,953 - -0.5 5,239,953 - +0.5 5,239,953 - +0.5 5,239,953 - -0.4 5,239,953 - +0.5 5,239,976 + -0.5 5,239,976 + -0.5 5,239,976 + -1.5 5,239,977 - +0.5 5,239,980 + +0.5 5,239,980 + -0.5 5,239,980 + +0.5 5,239,980 + -1.0 5,239,980 + +0.5 5,239,981 - -0.3 5,239,981 - +0.5 5,239,981 - -0.1 5,239,981 - -0.7 5,239,981 - +0.5 5,240,021 - -0.1 5,240,021 - +0.5 5,240,021 - +0.5 5,240,021 - +0.5 5,240,039 + -1.0 5,240,040 - +0.5 5,240,040 - +0.5 5,240,067 - -0.5 5,240,099 + -0.5 5,240,099 + -0.4 5,240,099 + -0.5 5,240,099 + -1.8 5,240,099 + -1.5 5,240,100 - +1.5 5,240,100 - -0.5 5,240,100 - -0.8 5,240,100 - +0.5 5,240,127 - +0.5 5,240,179 + -1.0 5,240,180 - -1.5 5,240,221 - +0.5 5,240,233 + -0.5 5,240,234 - -1.5 5,240,234 - -0.5 5,240,240 + +0.5 5,240,241 - -1.0 5,240,241 - -1.5 5,240,248 + +0.5 5,240,248 + +0.5 5,240,248 + -0.5 5,240,257 + -0.3 5,240,258 - +0.5 5,240,258 - +0.5 5,240,258 - +0.5 5,240,321 + +0.5 5,240,321 + -1.0 5,240,322 - -1.0 5,240,322 - +0.5 5,240,322 - -1.5 5,240,329 + -2.3 5,240,344 + +0.2 5,240,344 + -0.5 5,240,345 - -0.5 5,240,345 - +0.5 5,240,345 - +0.5 5,240,425 + Pf6N2E2_4509 0.15 -1.5 5,240,425 + Pf6N2E2_4509 0.15 -0.7 5,240,425 + Pf6N2E2_4509 0.15 -1.0 5,240,426 - Pf6N2E2_4509 0.15 -1.0 5,240,426 - Pf6N2E2_4509 0.15 -0.5 5,240,513 - Pf6N2E2_4509 0.22 -1.0 5,240,632 + Pf6N2E2_4509 0.31 -0.6 5,240,633 - Pf6N2E2_4509 0.32 -0.5 5,240,633 - Pf6N2E2_4509 0.32 -0.4 5,240,657 - Pf6N2E2_4509 0.33 -0.5 5,240,657 - Pf6N2E2_4509 0.33 -0.1 5,240,784 - Pf6N2E2_4509 0.43 +0.5 5,240,784 - Pf6N2E2_4509 0.43 -1.8 5,240,872 + Pf6N2E2_4509 0.50 +0.5 5,240,911 + Pf6N2E2_4509 0.53 +0.5 5,240,911 + Pf6N2E2_4509 0.53 -2.0 5,240,911 + Pf6N2E2_4509 0.53 -0.5
Or see this region's nucleotide sequence