Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4404 and Pf6N2E2_4405 are separated by 265 nucleotides Pf6N2E2_4405 and Pf6N2E2_4406 are separated by 7 nucleotides
Pf6N2E2_4404 - MG(2+) CHELATASE FAMILY PROTEIN / ComM-related protein, at 5,136,436 to 5,137,929
_4404
Pf6N2E2_4405 - sensor histidine kinase, at 5,138,195 to 5,139,472
_4405
Pf6N2E2_4406 - 2-Keto-D-gluconate dehydrogenase (EC 1.1.99.4), membrane-bound, flavoprotein, at 5,139,480 to 5,141,114
_4406
Position (kb)
5138
5139
5140 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5137.348 kb on + strand, within Pf6N2E2_4404 at 5137.348 kb on + strand, within Pf6N2E2_4404 at 5137.348 kb on + strand, within Pf6N2E2_4404 at 5137.348 kb on + strand, within Pf6N2E2_4404 at 5137.349 kb on - strand, within Pf6N2E2_4404 at 5137.349 kb on - strand, within Pf6N2E2_4404 at 5137.349 kb on - strand, within Pf6N2E2_4404 at 5137.349 kb on - strand, within Pf6N2E2_4404 at 5137.349 kb on - strand, within Pf6N2E2_4404 at 5137.349 kb on - strand, within Pf6N2E2_4404 at 5137.894 kb on + strand at 5137.895 kb on - strand at 5137.895 kb on - strand at 5137.980 kb on + strand at 5137.980 kb on + strand at 5137.981 kb on - strand at 5138.135 kb on + strand at 5138.135 kb on + strand at 5138.136 kb on - strand at 5138.136 kb on - strand at 5138.252 kb on + strand at 5138.252 kb on + strand at 5138.252 kb on + strand at 5138.252 kb on + strand at 5138.252 kb on + strand at 5138.252 kb on + strand at 5138.252 kb on + strand at 5138.252 kb on + strand at 5138.252 kb on + strand at 5138.252 kb on + strand at 5138.252 kb on + strand at 5138.253 kb on - strand at 5138.253 kb on - strand at 5138.253 kb on - strand at 5138.253 kb on - strand at 5138.315 kb on + strand at 5138.315 kb on + strand at 5138.315 kb on + strand at 5138.315 kb on + strand at 5138.315 kb on + strand at 5138.316 kb on - strand at 5138.316 kb on - strand at 5138.316 kb on - strand at 5138.316 kb on - strand at 5138.342 kb on + strand, within Pf6N2E2_4405 at 5138.342 kb on + strand, within Pf6N2E2_4405 at 5138.342 kb on + strand, within Pf6N2E2_4405 at 5138.342 kb on + strand, within Pf6N2E2_4405 at 5138.343 kb on - strand, within Pf6N2E2_4405 at 5138.343 kb on - strand, within Pf6N2E2_4405 at 5138.343 kb on - strand, within Pf6N2E2_4405 at 5138.459 kb on + strand, within Pf6N2E2_4405 at 5138.459 kb on + strand, within Pf6N2E2_4405 at 5138.459 kb on + strand, within Pf6N2E2_4405 at 5138.459 kb on + strand, within Pf6N2E2_4405 at 5138.459 kb on + strand, within Pf6N2E2_4405 at 5138.459 kb on + strand, within Pf6N2E2_4405 at 5138.460 kb on - strand, within Pf6N2E2_4405 at 5138.460 kb on - strand, within Pf6N2E2_4405 at 5138.460 kb on - strand, within Pf6N2E2_4405 at 5138.460 kb on - strand, within Pf6N2E2_4405 at 5138.460 kb on - strand, within Pf6N2E2_4405 at 5138.460 kb on - strand, within Pf6N2E2_4405 at 5138.460 kb on - strand, within Pf6N2E2_4405 at 5138.462 kb on + strand, within Pf6N2E2_4405 at 5138.462 kb on + strand, within Pf6N2E2_4405 at 5138.463 kb on - strand, within Pf6N2E2_4405 at 5138.463 kb on - strand, within Pf6N2E2_4405 at 5138.720 kb on + strand, within Pf6N2E2_4405 at 5138.720 kb on + strand, within Pf6N2E2_4405 at 5138.843 kb on + strand, within Pf6N2E2_4405 at 5138.843 kb on + strand, within Pf6N2E2_4405 at 5138.843 kb on + strand, within Pf6N2E2_4405 at 5138.844 kb on - strand, within Pf6N2E2_4405 at 5139.082 kb on + strand, within Pf6N2E2_4405 at 5139.083 kb on - strand, within Pf6N2E2_4405 at 5139.083 kb on - strand, within Pf6N2E2_4405 at 5139.085 kb on - strand, within Pf6N2E2_4405 at 5139.085 kb on - strand, within Pf6N2E2_4405 at 5139.340 kb on + strand, within Pf6N2E2_4405 at 5139.340 kb on + strand, within Pf6N2E2_4405 at 5139.341 kb on - strand, within Pf6N2E2_4405 at 5139.341 kb on - strand, within Pf6N2E2_4405 at 5139.341 kb on - strand, within Pf6N2E2_4405 at 5139.341 kb on - strand, within Pf6N2E2_4405 at 5139.412 kb on + strand at 5139.481 kb on + strand at 5139.779 kb on - strand, within Pf6N2E2_4406 at 5139.794 kb on - strand, within Pf6N2E2_4406 at 5139.794 kb on - strand, within Pf6N2E2_4406 at 5139.794 kb on - strand, within Pf6N2E2_4406 at 5139.796 kb on + strand, within Pf6N2E2_4406 at 5139.796 kb on + strand, within Pf6N2E2_4406 at 5139.796 kb on + strand, within Pf6N2E2_4406 at 5139.796 kb on + strand, within Pf6N2E2_4406 at 5139.797 kb on - strand, within Pf6N2E2_4406 at 5139.797 kb on - strand, within Pf6N2E2_4406 at 5139.797 kb on - strand, within Pf6N2E2_4406 at 5140.015 kb on + strand, within Pf6N2E2_4406 at 5140.015 kb on + strand, within Pf6N2E2_4406 at 5140.016 kb on - strand, within Pf6N2E2_4406 at 5140.016 kb on - strand, within Pf6N2E2_4406 at 5140.016 kb on - strand, within Pf6N2E2_4406 at 5140.016 kb on - strand, within Pf6N2E2_4406 at 5140.061 kb on - strand, within Pf6N2E2_4406 at 5140.061 kb on - strand, within Pf6N2E2_4406 at 5140.170 kb on - strand, within Pf6N2E2_4406 at 5140.170 kb on - strand, within Pf6N2E2_4406 at 5140.238 kb on - strand, within Pf6N2E2_4406 at 5140.238 kb on - strand, within Pf6N2E2_4406 at 5140.238 kb on - strand, within Pf6N2E2_4406 at 5140.255 kb on + strand, within Pf6N2E2_4406 at 5140.255 kb on - strand, within Pf6N2E2_4406 at 5140.297 kb on + strand, within Pf6N2E2_4406 at 5140.297 kb on + strand, within Pf6N2E2_4406 at 5140.298 kb on - strand, within Pf6N2E2_4406 at 5140.298 kb on - strand, within Pf6N2E2_4406 at 5140.394 kb on + strand, within Pf6N2E2_4406 at 5140.394 kb on + strand, within Pf6N2E2_4406 at 5140.395 kb on - strand, within Pf6N2E2_4406 at 5140.411 kb on + strand at 5140.411 kb on + strand, within Pf6N2E2_4406 at 5140.412 kb on - strand, within Pf6N2E2_4406 at 5140.412 kb on - strand, within Pf6N2E2_4406
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 5,137,348 + Pf6N2E2_4404 0.61 +0.5 5,137,348 + Pf6N2E2_4404 0.61 +0.5 5,137,348 + Pf6N2E2_4404 0.61 +0.5 5,137,348 + Pf6N2E2_4404 0.61 +0.5 5,137,349 - Pf6N2E2_4404 0.61 -1.3 5,137,349 - Pf6N2E2_4404 0.61 +0.5 5,137,349 - Pf6N2E2_4404 0.61 +0.5 5,137,349 - Pf6N2E2_4404 0.61 -0.5 5,137,349 - Pf6N2E2_4404 0.61 -1.7 5,137,349 - Pf6N2E2_4404 0.61 -1.5 5,137,894 + +0.5 5,137,895 - -0.5 5,137,895 - +0.5 5,137,980 + -1.0 5,137,980 + +0.5 5,137,981 - +0.4 5,138,135 + +0.5 5,138,135 + -0.0 5,138,136 - +0.6 5,138,136 - +0.5 5,138,252 + -1.8 5,138,252 + -1.8 5,138,252 + -0.5 5,138,252 + +0.5 5,138,252 + -0.5 5,138,252 + -0.6 5,138,252 + +0.5 5,138,252 + -1.0 5,138,252 + +0.5 5,138,252 + -1.0 5,138,252 + -0.5 5,138,253 - +0.3 5,138,253 - +2.1 5,138,253 - +0.1 5,138,253 - +0.1 5,138,315 + -1.0 5,138,315 + -0.5 5,138,315 + -0.5 5,138,315 + -0.5 5,138,315 + -1.0 5,138,316 - +0.3 5,138,316 - -0.2 5,138,316 - +1.8 5,138,316 - -0.6 5,138,342 + Pf6N2E2_4405 0.12 +0.5 5,138,342 + Pf6N2E2_4405 0.12 -0.5 5,138,342 + Pf6N2E2_4405 0.12 +1.9 5,138,342 + Pf6N2E2_4405 0.12 -2.3 5,138,343 - Pf6N2E2_4405 0.12 +0.9 5,138,343 - Pf6N2E2_4405 0.12 +0.4 5,138,343 - Pf6N2E2_4405 0.12 +0.3 5,138,459 + Pf6N2E2_4405 0.21 +0.5 5,138,459 + Pf6N2E2_4405 0.21 -2.5 5,138,459 + Pf6N2E2_4405 0.21 -0.6 5,138,459 + Pf6N2E2_4405 0.21 +0.5 5,138,459 + Pf6N2E2_4405 0.21 -0.5 5,138,459 + Pf6N2E2_4405 0.21 -0.5 5,138,460 - Pf6N2E2_4405 0.21 +0.2 5,138,460 - Pf6N2E2_4405 0.21 +0.3 5,138,460 - Pf6N2E2_4405 0.21 +0.3 5,138,460 - Pf6N2E2_4405 0.21 -0.3 5,138,460 - Pf6N2E2_4405 0.21 -0.3 5,138,460 - Pf6N2E2_4405 0.21 +0.2 5,138,460 - Pf6N2E2_4405 0.21 +0.5 5,138,462 + Pf6N2E2_4405 0.21 -0.3 5,138,462 + Pf6N2E2_4405 0.21 +0.5 5,138,463 - Pf6N2E2_4405 0.21 +0.5 5,138,463 - Pf6N2E2_4405 0.21 +0.2 5,138,720 + Pf6N2E2_4405 0.41 -0.5 5,138,720 + Pf6N2E2_4405 0.41 -0.5 5,138,843 + Pf6N2E2_4405 0.51 -0.5 5,138,843 + Pf6N2E2_4405 0.51 +0.5 5,138,843 + Pf6N2E2_4405 0.51 +0.5 5,138,844 - Pf6N2E2_4405 0.51 -0.0 5,139,082 + Pf6N2E2_4405 0.69 -0.8 5,139,083 - Pf6N2E2_4405 0.69 -0.5 5,139,083 - Pf6N2E2_4405 0.69 -0.5 5,139,085 - Pf6N2E2_4405 0.70 +0.2 5,139,085 - Pf6N2E2_4405 0.70 -0.5 5,139,340 + Pf6N2E2_4405 0.90 -0.5 5,139,340 + Pf6N2E2_4405 0.90 -1.0 5,139,341 - Pf6N2E2_4405 0.90 +0.5 5,139,341 - Pf6N2E2_4405 0.90 -0.5 5,139,341 - Pf6N2E2_4405 0.90 +0.5 5,139,341 - Pf6N2E2_4405 0.90 -0.5 5,139,412 + +0.5 5,139,481 + +0.5 5,139,779 - Pf6N2E2_4406 0.18 -0.3 5,139,794 - Pf6N2E2_4406 0.19 -0.0 5,139,794 - Pf6N2E2_4406 0.19 +0.5 5,139,794 - Pf6N2E2_4406 0.19 +0.5 5,139,796 + Pf6N2E2_4406 0.19 -0.5 5,139,796 + Pf6N2E2_4406 0.19 -1.0 5,139,796 + Pf6N2E2_4406 0.19 +0.5 5,139,796 + Pf6N2E2_4406 0.19 -0.5 5,139,797 - Pf6N2E2_4406 0.19 -1.5 5,139,797 - Pf6N2E2_4406 0.19 +0.5 5,139,797 - Pf6N2E2_4406 0.19 -1.8 5,140,015 + Pf6N2E2_4406 0.33 -0.7 5,140,015 + Pf6N2E2_4406 0.33 -2.0 5,140,016 - Pf6N2E2_4406 0.33 -0.7 5,140,016 - Pf6N2E2_4406 0.33 -1.4 5,140,016 - Pf6N2E2_4406 0.33 -1.1 5,140,016 - Pf6N2E2_4406 0.33 +0.5 5,140,061 - Pf6N2E2_4406 0.36 +0.5 5,140,061 - Pf6N2E2_4406 0.36 -0.6 5,140,170 - Pf6N2E2_4406 0.42 -0.9 5,140,170 - Pf6N2E2_4406 0.42 -0.5 5,140,238 - Pf6N2E2_4406 0.46 -0.6 5,140,238 - Pf6N2E2_4406 0.46 -0.5 5,140,238 - Pf6N2E2_4406 0.46 -1.0 5,140,255 + Pf6N2E2_4406 0.47 -0.3 5,140,255 - Pf6N2E2_4406 0.47 -0.3 5,140,297 + Pf6N2E2_4406 0.50 -0.4 5,140,297 + Pf6N2E2_4406 0.50 +0.5 5,140,298 - Pf6N2E2_4406 0.50 -0.5 5,140,298 - Pf6N2E2_4406 0.50 -1.6 5,140,394 + Pf6N2E2_4406 0.56 +0.5 5,140,394 + Pf6N2E2_4406 0.56 -1.0 5,140,395 - Pf6N2E2_4406 0.56 -0.5 5,140,411 + -0.8 5,140,411 + Pf6N2E2_4406 0.57 -1.5 5,140,412 - Pf6N2E2_4406 0.57 -1.4 5,140,412 - Pf6N2E2_4406 0.57 -1.5
Or see this region's nucleotide sequence