Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_355

Experiment: Ying_AminoAcid20 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_354 and Pf6N2E2_355 are separated by 152 nucleotidesPf6N2E2_355 and Pf6N2E2_356 are separated by 126 nucleotides Pf6N2E2_354 - Type III PLP / low-specificity D-threonine aldolase, at 419,632 to 420,711 _354 Pf6N2E2_355 - Transcriptional regulator, LysR family, at 420,864 to 421,748 _355 Pf6N2E2_356 - hypothetical protein, at 421,875 to 422,822 _356 Position (kb) 420 421 422Strain fitness (log2 ratio) -2 -1 0 1at 420.059 kb on + strand, within Pf6N2E2_354at 420.059 kb on + strand, within Pf6N2E2_354at 420.059 kb on + strand, within Pf6N2E2_354at 420.059 kb on + strand, within Pf6N2E2_354at 420.059 kb on + strand, within Pf6N2E2_354at 420.060 kb on - strand, within Pf6N2E2_354at 420.060 kb on - strand, within Pf6N2E2_354at 420.060 kb on - strand, within Pf6N2E2_354at 420.242 kb on + strand, within Pf6N2E2_354at 420.242 kb on + strand, within Pf6N2E2_354at 420.243 kb on - strand, within Pf6N2E2_354at 420.521 kb on + strand, within Pf6N2E2_354at 420.522 kb on - strand, within Pf6N2E2_354at 420.522 kb on - strandat 420.700 kb on - strandat 420.721 kb on - strandat 420.804 kb on + strandat 420.804 kb on + strandat 420.805 kb on - strandat 421.225 kb on + strand, within Pf6N2E2_355at 421.225 kb on + strand, within Pf6N2E2_355at 421.225 kb on + strand, within Pf6N2E2_355at 421.225 kb on + strand, within Pf6N2E2_355at 421.225 kb on + strand, within Pf6N2E2_355at 421.226 kb on - strand, within Pf6N2E2_355at 421.226 kb on - strand, within Pf6N2E2_355at 421.427 kb on + strand, within Pf6N2E2_355at 421.427 kb on + strand, within Pf6N2E2_355at 421.427 kb on + strand, within Pf6N2E2_355at 421.427 kb on + strand, within Pf6N2E2_355at 421.427 kb on + strand, within Pf6N2E2_355at 421.428 kb on - strand, within Pf6N2E2_355at 421.428 kb on - strand, within Pf6N2E2_355at 421.857 kb on - strandat 421.857 kb on - strandat 421.857 kb on - strandat 421.857 kb on - strandat 421.877 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3
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420,059 + Pf6N2E2_354 0.40 -0.7
420,059 + Pf6N2E2_354 0.40 +0.3
420,059 + Pf6N2E2_354 0.40 -0.7
420,059 + Pf6N2E2_354 0.40 +0.3
420,059 + Pf6N2E2_354 0.40 +0.3
420,060 - Pf6N2E2_354 0.40 +0.3
420,060 - Pf6N2E2_354 0.40 -0.4
420,060 - Pf6N2E2_354 0.40 -0.3
420,242 + Pf6N2E2_354 0.56 +0.3
420,242 + Pf6N2E2_354 0.56 +1.3
420,243 - Pf6N2E2_354 0.57 -1.7
420,521 + Pf6N2E2_354 0.82 +0.3
420,522 - Pf6N2E2_354 0.82 -0.7
420,522 - +0.3
420,700 - +0.3
420,721 - +0.3
420,804 + -0.7
420,804 + +0.3
420,805 - +0.3
421,225 + Pf6N2E2_355 0.41 +0.7
421,225 + Pf6N2E2_355 0.41 +0.3
421,225 + Pf6N2E2_355 0.41 -2.0
421,225 + Pf6N2E2_355 0.41 +0.3
421,225 + Pf6N2E2_355 0.41 +0.3
421,226 - Pf6N2E2_355 0.41 +0.3
421,226 - Pf6N2E2_355 0.41 -0.7
421,427 + Pf6N2E2_355 0.64 -0.7
421,427 + Pf6N2E2_355 0.64 -0.4
421,427 + Pf6N2E2_355 0.64 +0.3
421,427 + Pf6N2E2_355 0.64 +0.3
421,427 + Pf6N2E2_355 0.64 -0.7
421,428 - Pf6N2E2_355 0.64 +0.6
421,428 - Pf6N2E2_355 0.64 -1.3
421,857 - -0.7
421,857 - +0.3
421,857 - +1.3
421,857 - -2.3
421,877 - +0.3

Or see this region's nucleotide sequence