Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3365

Experiment: Ying_AminoAcid20 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3364 and Pf6N2E2_3365 are separated by 105 nucleotidesPf6N2E2_3365 and Pf6N2E2_3366 are separated by 255 nucleotides Pf6N2E2_3364 - Carbamoyl-phosphate synthase large chain (EC 6.3.5.5), at 3,893,736 to 3,896,957 _3364 Pf6N2E2_3365 - Carbamoyl-phosphate synthase small chain (EC 6.3.5.5), at 3,897,063 to 3,898,199 _3365 Pf6N2E2_3366 - 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8), at 3,898,455 to 3,899,261 _3366 Position (kb) 3897 3898 3899Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3896.876 kb on - strandat 3897.611 kb on - strand, within Pf6N2E2_3365at 3898.213 kb on - strandat 3898.217 kb on - strandat 3898.217 kb on - strandat 3898.217 kb on - strandat 3898.217 kb on - strandat 3898.263 kb on - strandat 3898.295 kb on - strandat 3898.412 kb on + strandat 3898.413 kb on - strandat 3898.427 kb on + strandat 3898.427 kb on + strandat 3898.428 kb on - strandat 3898.428 kb on - strandat 3898.428 kb on - strandat 3898.428 kb on - strandat 3898.430 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3
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3,896,876 - -0.6
3,897,611 - Pf6N2E2_3365 0.48 -0.6
3,898,213 - -1.9
3,898,217 - -1.9
3,898,217 - -2.4
3,898,217 - -1.9
3,898,217 - -2.5
3,898,263 - -1.6
3,898,295 - -4.0
3,898,412 + -0.6
3,898,413 - +0.4
3,898,427 + +0.4
3,898,427 + -0.6
3,898,428 - -0.7
3,898,428 - -0.6
3,898,428 - +0.4
3,898,428 - -0.6
3,898,430 - -1.6

Or see this region's nucleotide sequence